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김성민,석귀덕,Kim, Sun-Min,Suk, Kui-Duk 대한약학회 2007 약학회지 Vol.51 No.6
Thymus magnus is an endemic (Ulleung Island) species in Korea. This plant is used as diaphoretics and carminatives in traditional medicine. In the literature, few scientific assays were realized on this species, such as antibiotic (Streptococcus pneumoniae, Staphylococcus aureus, Salmonella enteritidis, and S. typhimurium) and antifungal activities. In order to clarify whether essential oil of T. magnus have pharmacological effects, anti-inflammatory, sedative, anti-depressant, analgesic, and sleep-prolonged effects were investigated using animal models. From this study, the following conclusions were attained; 1) Essential oil of T. magnus did not show any acute toxicity on mice when orally administered at the dose of 2-3 g/kg body weight. 2) Essential oil of T. magnus possessed strong anti-inflammatory activity, similar to that of a positive control prednisolone. 3) Essential oil of T. magnus had excellent analgesic activity, comparable to that of aspirin. 4) The essential oil of T. magnus possessed strong sleep-prolonged effect on pentobarbital induced-sleep test in mice model. 5) In the hot plate test, the essential oil of T. magnus had moderate effect. 6) And the essential oil of T. magnus had no significant effects in forced-swimming test and open-field test.
새삼, 실새삼 및 갯실새삼 추출물이 Clone M-3 세포주의 Melanin 생합성 및 Tyrosinase
장수진(Soo-Jin Chang),석귀덕(Kui-Duk Suk) 대한약학회 2006 약학회지 Vol.50 No.6
Water extracts, ethanol extracts, and juice of Cuscuta japunica, C. australis, and C. chinensis were prepared, and their cytotoxicity, antioxidant activity, and inhibitory effects on tyrosinase activity and melanin biosynthesis were estimated by using melanoma Clone M-3. From this study, the following conclusions were attained. Extracts of Cuscuta japonica, C. australis, and C. chinensis showed noticeable cytotoxicity except ethanol extracts from the stem of C. australis A maximual cytotoxicity was observed with the ethanol extract from the seed of C. australis (87.39%). While the ethanol extract form the seed of C. japonica (91.88%) showed the most pronounced inhibitory effect on melanin biosynthesis, the water extract from the stem of C. japonica (1.05%) possessed very little inhibitory effect. The most inhibitory effect on tyrosinase activity was observed with the water extract form the stem of C. australis (76.6%). However, the water extract from the stem of C. japonica showed a very poor effect on the inhibition of tyrosinase activity. All the preparations, except extracts from the seed of C. australis were able to remove reactive oxygen species (ROS) in a done-dependent manner. The juice of C. japonica demonstrated the strongest activity (59.02%).
송지의 세포독성과 항산화작용 및 arachidonic acid 생성 억제작용
조경미(Kyoung Mi Cho),석귀덕(Kui Duk Suk) 대한약학회 2002 약학회지 Vol.46 No.5
In this report, anti-inf1ammatory, analgesic and cytotoxic effects of the water extracts of Pini Resina, which has been as an additive to oral hygienic products together with sodium chloride in community were investigated. The water extracts of Pini Resina, pretreated Pini Resina and Ramus Mori Albae-added Pini Resina all showed relatively low cytotoxicity All samples showed concentration-dependent increase in electron-donating capacity to DPPH, especially, Ramus Mori Albae-added Pini Resina was the highest in the extent. Arachidonic acid release from the cell membrane was significantly inhibited by the presence of the samples above, among those, Ramus Mori Albae-added Pini Resina was the most effective in the inhibitory action of the release.
家兎眼 망막 색소상피세포 Acid phosphatase : 정제 및 활성에 미치는 인자들에 관한 연구 Purification and Characterization
석귀덕,송지영 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.5 No.-
Acid phosphatase [Orthophosphoric monoester phosphohydrolase(acid optimum), EC 3.1.3.2, ACPase] was purified from the retinal pigment epithelium of rabbit eyes near homogeneity. The purification procedure included lysosomal fractionation, acid treatment, ammonium sulfate fractionation, gel filtration chromatography on sephadex G-100 and ion exchange chromatography on DEAE-cellulose. ACPase was purified about 47 fold and it is mollecular weight was estimated to be approximately 126 kDa, and consists of two identical subunits(54 kDa) by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimum temperature of ACPase activity determined with p-nitirophenyl phosphate was 60℃. ACPase was inhibited by L-(+)-tartarate, fluoride, cupric ion, zinc ion and mercuric ion, whereas activated by magnesium ion, reducing agents(cystein, 2-mercaptoethanol or ascorbic acid) and EDTA. Although the enzyme appears to be the most activity toward P-nitrophenyl phosphate, marked activity was observed with Glucose-1-phosphate. ATP, ADP and AMP were not hydrolyzed by ACPase.