철강 제조업에서의 유해,위험구역 출입 관리 방안

서성화 ( Seong Hwa Seo ),김민 ( Min Kim ),원종일 ( Jong Il Weon ),우흥식 ( Heung Sik Woo ) 한국안전학회(구 한국산업안전학회) 2011 한국안전학회지 Vol.26 No.3

Access-control of hazard zone in a steel manufacturing industry is studied in terms of safety management. Based on the results of risk evaluation for hazard zone, three risk zones with low, middle and high level are categorized. These zones have different color door and locking shape depending on their risk levels. At the high level, red door and key-based locking system are employed to accessed-controled path. Furthermore, tagout, lockout, interlock system for emergency stop, warning and flashing are also introduced. New standardized procedure of access-control for various hazard zones, which could help to greatly contribute to the prevention of accidents in advance, is proposed considering the risk level and the condition of given hazard zones. The standardized procedure of access- management suggested in this study will take an effective role as one of safety guide lines for hazardous workshop of manufacturing industries.

4M 위험성평가 기법을 이용한 앗차사고의 효과적인 발굴기법

서성화 ( Seong Hwa Seo ),원종일 ( Jong Ii Weon ),우홍식 ( Heung Sik Woo ) 한국안전학회(구 한국산업안전학회) 2012 한국안전학회지 Vol.27 No.5

In this study, a new technique for detecting near miss using 4M risk assessment method is suggested. Until now, the safety education with instances of near miss has just been progressed in most industrial settings, without any systematic guideline. By menas of appling 4M risk assessment method, the organized technique, which could effectively manage the fundamental prevention of industrial accident in advance, is developed. The organized technique of near miss-management suggested in this study will take an effective role in basically expanding the application of risk assessment method, as well as in contributing the activity of zero-accident as a safety guideline in hazardous workshops.

철강 제조업의 플랜트 공사 중 유해.위험 저감 방안

서성화,원종일,우흥식,Seo, Seong-Hwa,Weon, Jong-Il,Woo, Heung-Sik 대한안전경영과학회 2011 대한안전경영과학회지 Vol.13 No.4

The hazard conditions on plant works of the steel industry have been investigated to efficiently prevent from industrial accidents. The plant works are eventually carried out by workers of smaller service companies where the safety management systems are not well established, on behalf of the main steel company. Based on the preliminary risk assessment on the plant working and open literature reviews, the safety management systems, which can be applied to the plant workplace of each process, are studied and then the step-by-step 4M check-list method is established. Accordingly, the risk assessment technique using 4M checklist for the plant workplace is proposed. In addition, a standardized flow-chart for safety management of plant work is introduced. These risk assessment technique and flow-chart can definitely contribute to the reduction of industrial accidents, considering the risk characterization and the unsafe conditions in small and medium-sized plant workplace, as effective safety management tools and safety guide line.

Improvement of Transglycosylation Efficiency using a Glycosynthase Mutant derived from Thermoplasma acidophilum ${\alpha}$-Glucosidase

황성민,서성화,박인명,최경화,김도만,차재호,Hwang, Sung-Min,Seo, Seong-Hwa,Park, In-Myoung,Choi, Kyoung-Hwa,Kim, Do-Man,Cha, Jae-Ho The Korean Society for Microbiology and Biotechnol 2012 한국미생물·생명공학회지 Vol.40 No.2

Glycosynthase는 친핵성 아미노산을 비친핵성 아미노산으로 치환하여 당전이 산물의 가수분해를 막아서 당전이 효율을 증가시킬 수 있다. 이전 연구에서 본 실험실은 열에 안정하고 산에 강한 Thermoplasma acidophilum 유래의 ${alpha}$-glucosidase (AglA)가 당전이 활성이 있음을 입증하였으나 시간이 지남에 따라 당전이 산물이 가수분해 되었다. 이러한 AglA의 당전이 효율을 개선하기 위하여 친핵성 아미노산인 아스파라긴산을 글리신으로 치환하였다. 이 치환된 glycosynthase는 니켈 친화력 크로마토그래피를 통하여 정제되었으며, 정제된 돌연변이 단백질의 배당체를 합성하는 능력이 말토오스를 공여체로 그리고 p-nitrophenyl-${alpha}$-D-glucopyranoside($pNP{\alpha}G$)를 수용체로, 그리고 $pNP{\alpha}G$가 당공여체 및 수용체로 이용될 수 있는지 검사하였다. Glycosynthase를 이용한 당전이 산물의 수율은 약 42.5%를 보였으며 시간이 지남에 따라서 가수분해되지 않았다. 박막 크로마토그래피법을 이용한 반응산물의 분석은 수용체의 높은 농도에서 기존의 효소보다 많은 양의 배당체를 합성할 수 있음을 보여주었고, 특히 중성보다 낮은 pH 영역에서 가장 높은 활성을 보여줌을 확인하였다. 이러한 결과는 glycosynthase가 산업적으로 배당체를 합성하는데 유용성이 크다는 것을 나타낸다. Glycosynthase is an active site nucleophile mutant enzyme, prepared from glycosidase, which is capable of synthesizing oligosaccharide derivatives without the hydrolysis of the product. Thermoacidophilic ${\alpha}$-glucosidase of Thermoplasma acidophilum (AglA) exhibits a transglycosylating activity yielding various glycosides. AglA was converted to glycosynthase by the substitution of the catalytic nucleophile Asp-408 residue into non-nucleophile glycine in order to increase its ability to synthesize various glycosides by transglycosylation. The glycosynthase mutant was purified by Ni-NTA chromatography and its glycoside-synthesizing activity was measured by using an external nucleophile, sodium formate buffer, providing maltose as a donor and p-nitrophenyl-${\alpha}$-D-glucopyranoside ($pNP{\alpha}G$) as an acceptor, respectively. In addition, $pNP{\alpha}G$ was examined for its feasibility to act as both a donor and an acceptor, and products were compared with those of the wildtype enzyme. The mutant enzyme was found to catalyze the formation of a specific product from $pNP{\alpha}G$ with a yield of 42.5% without further hydrolysis, while the wild-type enzyme produced two $pNP{\alpha}G$ products at low yields. The results demonstrate the possibility of satisfactory yields for the reactions in the presence of small amounts of acceptor, and demonstrate that the high activity of the mutant, at pHs below neutrality, was applicable in the transfer of glucose from the natural donor.

Thermoplasma acidophilum 유래 α-glucosidase로 부터 생산된 glycosynthase 돌연변이 단백질의 개선된 당전이 효율

황성민 ( Sung Min Hwang ),서성화 ( Seong Hwa Seo ),박인명 ( In Myoung Park ),최경화 ( Kyoung Hwa Choi ),김도만 ( Do Man Kim ),차재호 ( Jae Ho Cha ) 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 2012 한국미생물·생명공학회지 Vol.40 No.2

Glycosynthase는 친핵성 아미노산을 비친핵성 아미노산으로 치환하여 당전이 산물의 가수분해를 막아서 당전이 효율을 증가시킬 수 있다. 이전 연구에서 본 실험실은 열에 안정하고 산에 강한 Thermoplasma acidophilum 유래의 α-glucosidase (AglA)가 당전이 활성이 있음을 입증하였으나 시간이 지남에 따라 당전이 산물이 가수분해 되었다. 이러한 AglA의 당전이 효율을 개선하기 위하여 친핵성 아미노산인 아스파라긴산을 글리신으로 치환하였다. 이 치환된glycosynthase는 니켈 친화력 크로마토그래피를 통하여 정제되었으며, 정제된 돌연변이 단백질의 배당체를 합성하는 능력이 말토오스를 공여체로 그리고 p-nitrophenyl-α-D-glucopyranoside(pNPαG)를 수용체로, 그리고 pNPαG가 당공여체 및 수용체로 이용될 수 있는지 검사하였다. Glycosynthase를 이용한 당전이 산물의 수율은 약 42.5%를 보였으며 시간이 지남에 따라서 가수분해되지 않았다. 박막 크로마토그래피법을 이용한 반응산물의 분석은 수용체의 높은 농도에서 기존의 효소보다 많은 양의 배당체를 합성할 수 있음을 보여주었고, 특히 중성보다 낮은 pH 영역에서 가장 높은 활성을 보여줌을 확인하였다. 이러한 결과는 glycosynthase가 산업적으로 배당체를 합성하는데 유용성이 크다는 것을 나타낸다. Glycosynthase is an active site nucleophile mutant enzyme, prepared from glycosidase, which is capable of synthesizing oligosaccharide derivatives without the hydrolysis of the product. Thermoacidophilic α-glucosidase of Thermoplasma acidophilum (AglA) exhibits a transglycosylating activity yielding various glycosides. AglA was converted to glycosynthase by the substitution of the catalytic nucleophile Asp-408 residue into non-nucleophile glycine in order to increase its ability to synthesize various glycosides by transglycosylation. The glycosynthase mutant was purified by Ni-NTA chromatography and its glycoside-synthesizing activity was measured by using an external nucleophile, sodium formate buffer, providing maltose as a donor and pnitrophenyl- α-D-glucopyranoside (pNPαG) as an acceptor, respectively. In addition, pNPαG was examined for its feasibility to act as both a donor and an acceptor, and products were compared with those of the wildtype enzyme. The mutant enzyme was found to catalyze the formation of a specific product from pNPαG with a yield of 42.5% without further hydrolysis, while the wild-type enzyme produced two pNPαG products at low yields. The results demonstrate the possibility of satisfactory yields for the reactions in the presence of small amounts of acceptor, and demonstrate that the high activity of the mutant, at pHs below neutrality, was applicable in the transfer of glucose from the natural donor.