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Structural study of the Cytosolic C-terminus of Vanilloid Receptor 1
서민덕,원형식,오우택,이봉진 한국자기공명학회 2007 Journal of the Korean Magnetic Resonance Society Vol.11 No.2
Vanilloid receptor 1 [transient receptor potential vanilloid subfamily member 1 (TRPV1), also known as VR1] is a non-selective cationic channel activated by noxious heat, vanilloids, and acid, thereby causing pain. VR1 possesses six transmembrane domain and N- and C-terminus cytosolic domains, and appears to be a homotetramer. We studied the structural properties of C-terminus of VR1 (VR1C) using CD and NMR spectroscopy. DPC micelles, with a zwitterionic surface, and SDS micelles, with a negatively charged surface, were used as a membrane mimetic model system. Both SDS and DPC micelles could increase the stability of helical structures and/or reduce the aggregation form of the VR1C. However, the structural changing mode of the VR1C induced by the SDS and DPC micelles was different. The changes according to the various pHs were also different in two micelles conditions. Because the net charges of the SDS and DPC micelles are negative and neutral, respectively, we anticipate that this difference might affect the structure of the VR1C by electrostatic interaction between the surface of the VR1C and phospholipids of the detergent micelles. Based on these similarity and dissimilarity of changing aspects of the VR1C, it is supposed that the VR1C probably has the real pI value near the pH 7. Generally, mild extracellular acidic pH (6.5~6.8) potentiates VR1 channel activation by noxious heat and vanilloids, whereas acidic conditions directly activate the channel. The channel activation of the VR1 might be related to the structural change of VR1C caused by pH (electrostatic interactions), especially near the pH 7. By measuring the 1H-15N TROSY spectra of the VR1C, we could get more resolved and dispersed spectra at the low pH and/or detergent micelles conditions. We will try to do further NMR experiments in low pH with micelles conditions in order to get more information about the structure of VR1C.
이건섭,서민호,백성덕 대구보건대학 1994 대구보건대학 論文集 Vol.14 No.-
The development of recombinant DNA technology bring about a revolution in biology. In medicine molecular biology is widely appolicated to diagnosis, treatment and understanding of disease. For diagnosis of infectious disease, cancer and genetic disorder, recombinant DNA technology provides rapid, specific, sensitive new method ; Southern blot, Northern blot, in situ hybridization and PCR(Polymerase Chain Reaction), etc. PCR is being used for rapid and sensitive diagnostic method of infectious disease, for example for diagnosis fo Mycobacterium tuberculosis classic culture method needs 4~7 weeks but PCR only 1~2 days. Over the nest few years by combing automated method for DNA extraction, PCR, and non-radioactive labelling, it may be possible to automate most of steps in molecular diagnosis of disease.
백성덕 ( S D Paik ),서민호 ( M H Sub ),이건섭 ( G S Lee ) 대한임상검사과학회 1992 대한임상검사과학회지(KJCLS) Vol.24 No.1
The development of recombinant DNA technology bring about a revolution in biology. In medicine molecular biology is widely appolicated to diagnosis, treatment and understanding of disease. For diagnosis of infectious disease, cancer and genetic disorder, recombinant DNA technology provide.:; rapid, specific, sensitive new method ; Southern blot, Northern blot, in situ hybridization and PCR(Polymerase Chain Reaction), etc. PCR is being used for rapid and sensitive diagnostic method of infectious disease, for example for diagnosis fo Mycobacterium tuberculosis classic culture method needs 4 --7 weeks but PCR only 1-- 2 days. Over the next few years by combing automated method for DNA extraction, PCR, and non-radioactive labelling, it may be possible to automate most of steps in molecular diagnosis of disease.
원형식,서민덕,고현숙,최완수,이봉진 한국분자세포생물학회 2008 Molecules and cells Vol.26 No.1
Cyclic AMP receptor protein (CRP) is allosterically activated by cAMP and functions as a global transcription regulator in enteric bacteria. Structural information on CRP in the absence of cAMP (apo-CRP) is essential to fully understand its allosteric behavior. In this study we demonstrated interdomain interactions in apo-CRP, using a comparative thermodynamic approach to the intact protein and its isolated domains, which were prepared either by limited proteolysis or using recombinant DNA. Thermal denaturation of the intact apo-CRP, monitored by differential scanning calorimetry, revealed an apparently single cooperative transition with a slight asymmetry. Combined with circular dichroism and fluorescence analysis, the thermal denaturation of apo-CRP could be interpreted as a coupled process involving two individual transitions, each attributable to a structural domain. When isolated individually, both of the domains exhibited significantly altered thermal behavior, thus pointing to the existence of non-covalent interdomain interactions in the intact apo-CRP. These observations suggest that the allosteric conformational change of CRP upon binding to cAMP is achieved by perturbing or modifying pre-existing interdomain interactions. They also underline the effectiveness of a comparative approach using calorimetric and structural probes for studying the thermodynamics of a protein.
海水,魚貝類 및 生活下水에서의 Vibrio菌屬의 分離同定과 抗菌劑 感受性
金聖光,徐民濩,白成德 啓明大學校 醫科大學 1990 계명의대학술지 Vol.9 No.3
Vibrio species isolated from various marine enviromments during the period form April to SEptember 1989 in the East sea and the South sea of Korea were studied for species distribution and antibiotiv suseptibility test in vitro. The number of strains isolated were 221 among 324 total specimens, they were classified into V. parahaemolyticus (27.6%), V. alginloyticus (51.6%) and nonfermenting Vibrio species (20.8%), but V. choerae and V. vulnificus were not isolated. V. parahemolyticus were more frequently isolated from Kyungju gun (31.7%) among 41 specimens by regional groups an followed by Geojegun (26.7%) and Masan city (26.3%), and shellfish (8 among 28 specimens; 28.6%) were showed the higher isolation rate among specimens tested. V. alginolyticus were more frequentiy isolated from Kyungju gun (45.2%), and sea water (75 among 166 specimens) showed higher isolation rate than tee other specimens. Nonfermenting Vibrio species were more frequently isolated from Masan city than the other regions, and sea water (10 among 19 specimens;; 24.7%) showed higher isolation rate of this strains. During the period of this study, the increased temperature of sea witer was found to be a correlatioin with the increased frequency of isolation rate of Vibrio species. Among V. parahaemolyticus, all strains were rresistant to penicillin and ampicillin, 68.9% to carbenicillin, and only 3.3% to cephalothin, but no resistant strains to other drugs tested were found. MICs of cephalothin to most strains were 16-32㎍/㎖, thus the resistance criteria to this drug was not deremined.