http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
광합성세균 Rhodobater capsulatus PS-2의 대량배양 최적화 및 대사산물 분석
봉기문(Ki Moon Bong),김종민(Jong Min Kim),유재홍(Jae-Hong Yoo),박인철(In Chul Park),이철원(Chul Won Lee),김평일(Pyoung Il Kim) 한국생물공학회 2016 KSBB Journal Vol.31 No.3
Plant growth promoting (PGP) hormones, which are produced in a small quantity by bacteria, affect in plant growth and development. PGPs play an important role on the crop productivity in agricultural field. In this study, a photosynthetic bacterial strain producing the PGP was isolated from paddy soil. Bacterial isolate was gram negative, rod-shaped and motility positive. From the 16s rRNA gene sequence analysis, the isolate was identified as Rhodobacter capsulatus PS-2. The mass cultivation of R. capsulatus PS-2 was optimized by considering of the carbon, nitrogen and inorganic salt sources. Optimal medium composition was determined as Na-succinate 4.5 g, yeast extract 5 g, K2HPO4 1 g, MgSO4 5 g , per liter. From the result of 500 L fermentation for 2 days using the optimal medium, the viable cells were 8.7×109 cfu/mL. R. capsulatus PS-2 strain produced the carotenoid and indole-3-acetic acid (IAA). The carotenoid extraction and quantitative analysis were performed by HCl-assisting method. Total carotenoid contents from R. capsulatus PS-2 culture broth were measured as 7.02±0.04 and 6.93±0.05 mg/L under photoheterotrophic and chemoheterotrophic conditions, respectively. To measure the productivity of IAA, colorimetric method was employed using Salkowski reagent at optical density 535 nm. The results showed that the highest content of IAA was 197.44±5.92 mg/L in the optimal medium supplemented with 0.3% tryptophan.
황토로부터 분리한 Bacillus licheniformis의 항진균 chitinase 생산과 효소 특성
한귀환(Gui Hwan Han),봉기문(Ki Moon Bong),김종민(Jong Min Kim),김평일(Pyoung Il Kim),김시욱(Si Wouk Kim) 한국생물공학회 2014 KSBB Journal Vol.29 No.3
In this study, we isolated two novel chitinase producing bacterial strains from yellow loess samples collected from Jullanamdo province. The chitinase producing bacteria were isolated based on the zone size of clearance in the chitin agar plates. Both of them were gram positive, rod (2~3×0.3~0.4 μm), spore-forming, and motility positive. They were facultative anaerobic, catalase positive and hydrolyzed starch, gelatin, and casein. From the 16s rRNA gene sequence analysis, the isolates were labeled as Bacillus licheniformis KYLSCU01 and B. licheniformis KYLS-CU02. The isolates showed higher extracellular chitinase activities than B. licheniformis ATCC 14580 as a control. The optimum temperature and pH for chitinase production were 40oC and pH 7.0, respectively. Response Surface Methodology (RSM) was used to optimize the culture medium for efficient production of the chitinase. Under this optimal condition, 1.5 times higher chitinase activity of B. licheniformis KYLS-CU02 was obtained. Extracellular chitinases of the two isolates were purified through ammonium sulfate precipitation and anion-exchange DEAE-cellulose column chromatography. The specific activities of purified chitinase from B. licheniformis KYLS-CU01 and B. licheniformis KYLS-CU02 were 7.65 and 5.21 U/mg protein, respectively. The molecular weights of the two purified chitinases were 59 kDa. Further, the purified chitinase of B. licheniformis KYLS-CU01 showed high antifungal activity against Fusarium sp.. In conclusion, these two bacterial isolates can be used as a biopesticide to control pathogenic fungi.
한국의 일 지역 독거노인에서 종교성 및 영성이 우울 및 삶의 질에 미치는 영향
허윤(Yoon Huh),문유선(Yoo Sun Moon),손봉기(Bong Ki Son),이상규(Sang Kyu Lee),이강(Kang Lee),노현진(Hyun Jin Rho),김도훈(Do Hoon Kim) 대한노인정신의학회 2008 노인정신의학 Vol.12 No.2
Objectives : This study tried to investigate the effects of religiosity and spirituality on depression and quality of life in older people. Methods : This study was conducted by selecting 274 solitary elderly people of over 65 years of age living in Chuncheon city. Symptoms of depression were evaluated by SGDS-K (Short Geriatric Depression Scale of Korean version) and quality of life were measured by GQOL-D (Geriatric Quality of Life-Dementia). We used the Duke Religion Index (DUREL) to assess religiosity and spirituality. Results : There was significant correlation between scales of depression (SGDS-K), quality of life (GQOL-D) and the scale of religiosity, spirituality (DUREL) in older people. Depressed people had lower score in GQOL-D than non-depressed. Among the depressed, people believing in a religion had higher score in GQOL-D than people who have no religion. As a result of multiple regression analysis, we came to know that religiosity and spirituality had significant effects on depression and quality of life in elderly. Interestingly, religiosity and spirituality is not related with depression and quality of life in Buddhism, but in Christian and Catholic. Conclusion : Religiosity and spirituality had significant effects on depression and the quality of life in Korean elderly. However, there are different relationships between depression and religiosity, quality of life and religiosity according to different kinds of religion. In the future, more research is needed.
지역사회에 거주하고 있는 노인의 우울증과 삶의 질을 평가하는 도구로 한국판 WHO-5의 유용성
김현지(Hyun Ji Kim),문유선(Yoo Sun Moon),손봉기(Bong Ki Son),이상규(Sang Kyu Lee),노현진(Hyun Jin Rho),김도훈(Do Hoon Kim) 대한노인정신의학회 2010 노인정신의학 Vol.14 No.2
Background and Objectives : Recently the number of geriatric depressed people has been increasing tremendously. Among many factors affecting depression, quality of life seems to be closely related to depression. Thus, a screening tool for assessing both geriatric depression and quality of life is needed. The purpose of this study is to estimate the utility of the Korean version of the WHO-Five Well-Being Index (WHO-5) in evaluating geriatric depression as compared with SGDS-K (Short Geriatric Depression Scale of Korean version) that is being used widely and quality of life. Methods : A sample of 244 subjects over 65-year old living in Yanggu and Inje area in Gangwon Province, Korea were interviewed and responded to scales, including WHO-5, SGDS-K, MMSE-KC (Mini Mental Status Examination in the Korean version of the CERAD assessment packet) and GQOL-D (Geriatric Quality of Life-Dementia). The total score of WHO-5 below 13 indicates low well-being. Results : The WHO-5 inversely correlated with SGDS-K and positively with GQOL-D, but showed no significant correlation with MMSE-KC. The SGDS-K negatively correlated with WHO-5, MMSE-KC, and GQOL-D. Low well-being group (WHO-5 score<13) had lower GQOL-D score (t=1.9698, p<0.05) and higher SGDS-K score (t=1.9798, p<0.05) than high well-being group. In multiple regression analysis, WHO-5 was significantly associated with GQOL-D and SGDS-K (r2=0.29422, p=0.0000). Conclusion : The Korean version of WHO-5 was very useful to evaluate both depressive symptoms and quality of life in the aged dwelling in community.