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박화용,황윤환,장두례,하정호,정기연,마진열,Park, Hwayong,Hwang, Youn-Hwan,Jang, Doorye,Ha, Jeong-Ho,Jung, Kiyoun,Ma, Jin Yeul 대한한의학방제학회 2012 大韓韓醫學方劑學會誌 Vol.20 No.2
Objectives : Traditional medicine Gumiganghwal-tang (GT) has been used in Asia to treat inflammatory diseases including common cold, pain, fever, and algor. In this study we investigated the acute toxicity and safety of GT and fermented GT (FGT). Methods : Acute toxicity and safety were evaluated in male and female ICR mice orally administered 0 (control) and 2,000 mg/kg of GT and FGT. After the administration of GT and FGT, we observed mortality, body weight, clinical symptoms. After necropsy, organ weights were measured and blood analysis was performed. Results : There was no mortality and clinical symptoms according to the administration of GT and FGT. Comparing with control group, there were no significant alterations on the organ weight, complete blood cell count and biochemical parameters. Conclusions : Median lethal dose of GT and FGT considered to be over 2,000 mg/kg in both male and female mice, and recognized as safe with no toxicity.
박화용,황윤환,하정호,정기연,마진열,Park, Hwayong,Hwang, Youn-Hwan,Ha, Jeong-Ho,Jung, Kiyoun,Ma, Jin Yeul 대한한의학방제학회 2013 大韓韓醫學方劑學會誌 Vol.21 No.1
Objectives : Traditional medicine Oryeong-san (ORS) has been prescribed for a long time to treat light fever, thirst, dysuria, and accompanying edema. However, the acute toxicity and safety were not reported. In this study, we evaluated the potent acute toxicity and safety of ORS. Methods : ICR mice were used to evaluate acute toxicity and safety by oral administration of 0, 500, 1,000, 2,000mg/kg of ORS. Mortality, body weight, and clinical symptoms were observed, and organ weight and blood biochemical parameters were analyzed after necropsy. Results : We found no mortality and no toxic or abnormal clinical symptoms by administration of ORS. Comparing with control group, no significant alterations in organ weight and blood biochemical parameters were observed. Conclusions : ORS recognized as safe and non-toxic medicinal material, and median lethal dose considered to be over 2,000 mg/kg in both male and female ICR mice.
박화용(Hwayong Park),이지혜(Jihye Lee),조장원(Changwon Cho),마진열(Jinyeul Ma) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.3
In this research, the acute toxicity of fermented Yukmijihwangtang extract was examined using male and female ICR mice, To evaluate the acute toxity and safety, 0(control group), 1250, 2500 and 5000 ㎎/㎏ of fermented Yukmijihwangtang extract were orally administered to male and female ICR mice. After single administration, we observed survival rates, general toxicity, changes of body weight for the 14 days and autopsy at 1 day following the administration according to the Regulation of Korean Food and Drug Administration. Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 ㎎/㎏). LD?? of fermented Yukmijihwangtang extract might be over 5000 ㎎/㎏ and it is very safe to ICR mice.
영지버섯 균사체를 이용한 고체 발효 쌍화탕의 급성독성에 관한 연구
엄영란(Youngran Um),박화용(Hwayong Park),이재훈(Jaehoon Lee),심기석(Kisuck Shim),마진열(Jinyeul Ma) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.1
This study was carried out to investigate the acute toxicity and safety of Ssanghwa-tang extract fermented with Ganoderma lucidum. To evaluate the acute toxity and safety, 0(control group), 1250, 2500 and 5000 ㎎/㎏ of Ssanghwa-tang and fermented Ssanghwa-tang extracts were orally administered to 20 male and 20 female ICR mice. After single administration, we observed survival rates, general toxicity, changes of body weight, and autopsy. Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 ㎎/㎏). LD?? of Ssanghwa-tang and fermented Ssanghwa-tang extracts might be over 5000 ㎎/㎏ and it is very safe to ICR mice.
이지혜(Jihye Lee),엄영란(Youngran Um),박화용(Hwayong Park),이재훈(Jaehoon Lee),마진열(Jinyeul Ma) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.2
The purpose of this study was investigation of quantitative analysis of marker substances in Paeonia lactiflora extracts by solid fermentation. High performance liquid chromatography (HPLC) for the determination of albiflorin and paeoniflorin in P. lactiflora extracts by solid fermentation, the separation method was performed on C18 column (250 ㎜ × 4.6 ㎜, 5 ㎛, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (230㎚). The flow rate was 1.0 ㎖/min. Retention time of albiflorin and paeoniflorin was about 28.88, 31.92 min and linearity of calibration was showed good result(r2 = 0.9998, 0.9996), respectively. Content of albiflorin was 0.090 ± 0.03% in P. lactiflora extract(control), 0.102 ± 0.00% in P. lactiflora extract fermented with Paecilomyces japonica, 0.056 ± 0.01% in P. lactiflora extract fermented with Ganoderma lucidum, 0.093 ± 0.00% in P. lactiflora extract fermented with honey and 0.046 ± 0.00% in P. lactiflora extract fermented with Nuruk. Content of paeoniflorin was 4.506 ± 0.13% in control, 2.599 ± 0.04% in P. lactiflora extract fermented with Paecilomyces japonica, 1.222 ± 0.03% in P. lactiflora extract fermented with Ganoderma lucidum, 2.750 ± 0.05% in P. lactiflora extract fermented with honey and 0.847 ± 0.00% in P. lactiflora extract fermented with Nuruk, respectively. Content of the marker substances did not increase in all fermentation experiment group.
사염화탄소 처리한 SD(rat)에 대한 진균발효쌍화탕의 간독성 치료효과 연구
이재훈(Lee Jaehoon),마충제(Ma Choongje),하혜경(Ha Hyekyung),전원경(Jeon Wonkyung),박화용(Park Hwayong),마진열(Ma Jinyeul) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
In this research, as a method for verifying the efficacy of Ssangwhatang and fungus-fermentated Ssangwhatang, a comparative study on the liver protection effect was conducted using animal experiments by inducing the liver toxicity with the CG₁₄ treatment. Inducing the liver damage resulted in the increase in the serum AST and ALP activity, and one day administration of the test material(CCl₄: 0.5 ㎖/㎏/day) caused 520 IU/L of the ASP activity leading to 29% enhancement in comparison with the normal group and 93% and 81% reductions in the fungus-fermentated Ssangwhatang-administered groups, BFST1 and BFST2, respectively. ALT is 42 IU/L for the normal group and 99 IU/L for the negative control group leading to 135% enhancement. 15 ㎖/㎏/day and 30 ㎖/㎏/day administrations of fungus-fermented Ssangwhatang(BFST) resulted in 51% and 45% decreases in the ALT concentration, respectively. One day administration of 30 ㎖/㎏ Ssangwhatang and fungus-fermentated Ssangwhatang caused the LDH in the plasma to tend to decrease. CCl₄(1.0 ㎖/㎏/day) administered at the 0th and 4th days led to the observation of the tendency toward the decrease in AST, ALT, and LDH contents. The results indicate that the function of Ssangwhatang is partly reinforced under the fungus-fermentated Ssangwhatang performed in order to verify the efficacy of Ssangwhatang's effect on the recovery from fatigues.
엄영란(Youngran Um),심기석(Kishuk Shim),이재훈(Jaehoon Lee),박화용(Hwayong Park),마진열(Jinyeul Ma) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.1
The aim of this study was to study the quantitative analysis of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica, Ganoderma lucidum, honey or Nuruk. The amounts of dry on loss were measured and the quantitative analysis of glycyrrhizic acid was performed by high performance liquid chromatographic (HPLC). HPLC method was performed on C18 column (250 ㎜ × 4.6 ㎜, 5 ㎛, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (254 ㎚). The flow rate was 1.0 ㎖/min. Retention time of glycyrrhizic acid was about 23.96 min and linearity of calibration was R²=0.9998. Contents of glycyrrhizic acid in Glycyrrhizae Radix extract (control) was 5.048 ± 0.14; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica (SDT) was 1.975 ± 0.07; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Ganoderma lucidum (SYT) was 2.676 ± 0.07; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with honey (SST) was 5.191 ± 0.06; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Nuruk (SNT) was 5.305 ± 0.34, respectively. Contents of glycyrrhizic acid in SDT and SYT were decreased but that in SST and SNT was increased when compared to control.