혈관모사 마이크로채널이 장착된 3D 종양 세포 배양 시스템의 제작 및 검증 연구

박정연,고범석,김기영,이동목,윤길상,Park, Jeong-Yeon,Koh, Byum-seok,Kim, Ki-Young,Lee, Dong-Mok,Yoon, Gil-Sang 한국금형공학회 2021 한국금형공학회지 Vol.15 No.2

Recently, three-dimensional (3D) cell culture systems, which are superior to conventional two-dimensional (2D) vascular systems that mimic the in vivo environment, are being actively studied to reproduce drug responses and cell differentiation in organisms. Conventional two-dimensional cell culture methods (scaffold-based and non-scaffold-based) have a limited cell growth rate because the culture cannot supply the culture medium as consistently as microvessels. To solve this problem, we would like to propose a 3D culture system with an environment similar to living cells by continuously supplying the culture medium to the bottom of the 3D cell support. The 3D culture system is a structure in which microvascular structures are combined under a scaffold (agar, collagen, etc.) where cells can settle and grow. First, we have manufactured molds for the formation of four types of microvessel-mimicking chips: width / height ①100 ㎛ / 100 ㎛, ②100 ㎛ / 50 ㎛, ③ 150 ㎛ / 100 ㎛, and ④ 200 ㎛ / 100 ㎛. By injection molding, four types of microfluidic chips were made with GPPS (general purpose polystyrene), and a 100㎛-thick PDMS (polydimethylsiloxane) film was attached to the top of each microfluidic chip. As a result of observing the flow of the culture medium in the microchannel, it was confirmed that when the aspect ratio (height/width) of the microchannel is 1.5 or more, the fluid flows from the inlet to the outlet without a backflow phenomenon. In addition, the culture efficiency experiments of colorectal cancer cells (SW490) were performed in a 3D culture system in which PDMS films with different pore diameters (1/25/45 ㎛) were combined on a microfluidic chip. As a result, it was found that the cell growth rate increased up to 1.3 times and the cell death rate decreased by 71% as a result of the 3D culture system having a hole membrane with a diameter of 10 ㎛ or more compared to the conventional commercial. Based on the results of this study, it is possible to expand and build various 3D cell culture systems that can maximize cell culture efficiency by cell type by adjusting the shape of the microchannel, the size of the film hole, and the flow rate of the inlet.

실리콘 수지 혼합비율에 따른 Pre-form 형광체 막 특성 변화에 관한 연구

박정연,윤길상,진형준,Park, Jeong Yeon,Yoon, Gil Sang,Jin, Hyoung-Joon 한국섬유공학회 2016 한국섬유공학회지 Vol.53 No.1

The effect of the viscosity of phosphor slurry prepared by mixing two types of silicone resins with different viscosities in various ratios on the properties of pre-formed phosphor films was investigated. The pre-formed phosphor films manufactured using these phosphor slurries were evaluated for curing characteristics, breaking strength, shear strength, and optical properties. The test results demonstrated that increasing the amount of high-viscosity silicone resin in the composite resin resulted in decreased curing reaction rate, decreased breaking strength, and correlated color temperature, whereas the viscosity and adhesion strength of the composite resin increased. Among seven different mixing ratios of the low- and high-viscosity silicone resins prepared for the composite resin, white-light emission was realized for the ratio 70:30, and the pre-formed phosphor film with this ratio exhibited high breaking strength and low adhesion strength. The breaking strength thus obtained was 24.0 MPa, the adhesion strength was 0.17 MPa, the color temperature was 5,650 K, and the two chromaticity coordinates (CIE(x, y) (1931)) were (0.3293, 0.3251).

제네릭 의약품 약가 조정 고시에 대한 비판적 고찰

박정연,Park, Jeong Yeon 대한의료법학회 2021 의료법학 Vol.22 No.1

2019년 5월 식약처는 원료의약품 등록 및 자체 생동성 시험 요건 충족 여부에 따라 제네릭 의약품의 가격을 차등 산정하는 것을 내용으로 하는 「약제의 결정 및 조정 기준」 고시를 개정하였다. 이는 기허가 제네릭 의약품에 대해서조차 자체 생동성 시험을 시행하지 않는 경우 기존 약가를 인하하는 것으로서 과연 그 입법목적과 수단에 관한 충분한 공법적 고려 하에 도입된 것인가 하는 의문이 든다. 이 글은 실질적으로는 위탁·공동 생동 시험을 제한하고자 자체 생동성 여부를 기준으로 제네릭 의약품의 약가를 결정·조정하는 개정 고시의 배경을 소개한 후 입법목적의 정당성 및 비례원칙의 관점에서 이를 비판적으로 검토하였다. 첫째, 목적의 정당성 측면에서 제네릭 의약품 난립 방지라는 목적이 과연 그 입법목적으로서 정당한 것인지에 대한 검토가 부족하였다는 점을 지적하였다. 둘째, '자체 생동 요건 미충족시 약가 인하'라는 내용의 개정 고시가 적합성 원칙에 부합하려면 위탁·공동 생동으로는 안전성·유효성을 인정하기 어렵다거나 적어도 위탁·공동 생동이 자체 생동에 비해 안전성·유효성 검증에 있어서 불충분하다는 전제가 성립해야 한다. 그럼에도 이에 대한 검토가 이루어지지 않은 것으로 보이는바, 안전성·유효성 확보라는 목적 달성을 위해서는 자체 생동이냐 위탁·공동 생동이냐보다 '생동성 인정 기준 및 생동성 시험자체의 관리 강화'에 초점이 맞추어져야 한다. 셋째, 필요성 및 상당성 판단에 있어서 품목허가 후 상당한 기간이 지나 충분히 안전성·유효성에 대한 검증이 이루어졌다고 볼 수 있는 제품에 대해서까지 일률적으로 개정된 고시 기준을 적용하는 것은 필요성 및 상당성 원칙에 반할 소지가 있다고 판단하였다. 개정 고시와 같은 행정입법은 많은 경우 법리적 검토나 공감대 형성이 부족한 상태에서 제·개정이 이루어지는 반면, 그에 따른 규제 효과는 피규제자에게 상당히 직접적이고 구체적이다. 이러한 점에서 행정입법 과정에 대해서도 규제목적과 수단에 대한 실질적인 검토가 요구되면, 사전통제로서 이해관계인 등의 절차참여제도가 보완되어야 함을 강조하였다. In May 2019, the Ministry of Food and Drug Safety revised the "Pharmaceutical Determination and Adjustment Criteria" with the content of differentially calculating the price of generic drugs according to the registration of the drug substance and meeting the requirements for their own bioequivalence test. According to this revised rule, if their own bioequivalence test is not conducted, even the generic drugs that have already been approved would be lowered in price. I wondered whether this system was introduced with sufficient public legal considerations regarding its legislative purposes and means. Therefore, I reviewed the contents of the revised notice based on whether or not it is valid to determine and adjust the price of generic drugs in terms of the legitimacy of legislative purposes and the proportionality principle after introducing the history and background of the rule. First, I raised a question as to whether the purpose of preventing the overrun of generic drugs is indeed legitimate in terms of the legitimacy of the purpose. In order for the revised notice of "reduction of drug prices when the test requirements are not met," to meet the conformity principle, the premise that it is difficult to recognize safety and effectiveness through consignment (joint) bioequivalence test or that these tests are insufficient in safety and efficacy verification than their own test must be established. Nevertheless, it seems that suffficient review has not been carried out. In order to achieve the purpose of securing safety and effectiveness, the focus should be on 'reinforcement of the standards for bioequivalence test and the management of the bioequivalence test itself' rather than whether it is a their own test or a consignment (joint) test. Third, it is contrary to the necessity and substantiality principle that strict standards are uniformly applied to the products that can be considered to have been sufficiently verified for safety and effectiveness after a considerable period of time has passed after the product approval. In many cases, revised administrative legislations quickly enacted and amended in the state of lack of legal review or consensus, while the regulatory effects resulting from it are quite direct and specific to the regulated person. In this respect, I emphasized that the administrative legislative process also requires substantial review and prior control of the regulatory purposes and means, and that the participation of stakeholders in the legislative procedure is to be strengthened.

동일 유동거리 런너를 가진 LSR 성형품의 변형에 관한 연구

박정연,윤길상,이정원,최종명,Park, Jeong-Yeon,Yoon, Gil-Sang,Lee, Jeong-Won,Choi, Jong Myeong 한국금형공학회 2013 한국금형공학회지 Vol.7 No.1

Recently, Silicone that one of the thermo-sets is used to making optical products such as LED lenses because of excellent thermal properties. LED lenses are required to keep the precise dimensions, so they must be molded to have the minimum deformation. Thermo-sets have the expansion characteristic on the part of thermal property, it is important to optimize the cure condition so that the deformation of the part become minimum. In this study, to investigate the relationship between the shrinkage by the curing and expansion by the thermal properties of the resin, reactive injection experiment was performed by setting the variables such as mold setting temperature, cure time. As a result, it was confirmed that there was a interval while the thermal properties were transferred to more active during the cure process. It is expected to help in determining the reactive injection molding conditions of the thermo-set parts as well as LED lens in order to reduce the amount of deformation.

진료비청구 및 심사사례

박정연,Park, Jeong-Yeon 대한병원협회 1998 대한병원협회지 Vol.27 No.6

액체 종류에 따른 식물의 성장 특성 기초 실험

박정연,김정배,Park, Jeong-Yeon,Kim, Jeongbae 한국교통대학교 융복합기술연구소 2014 융ㆍ복합기술연구소 논문집 Vol.4 No.1

This study was experimentally performed to show the growth characteristics of one specified plant with various liquids, which can be purchased easily. To do that, this study measured the number of leaves attached to the plant sample and the height of liquids everyday during almost 15 days on summer season in the laboratory room. From the experiments, we revealed that the remained number of leaves can be related with the liquid height.

마이크로바이오칩의 전기신호검출 시스템에 관한 연구

박정연(Jeong Yeon Park),박재준(Jae Jun Park),권기환(Ki Hwan Kwon),조남규(Nahm Gyoo Cho),안유민(Yoo Min Ahn),이성환(Seoung Hwan Lee),황승용(Seung Yong Hwang) Korean Society for Precision Engineering 2005 한국정밀공학회지 Vol.22 No.4

In this study, a microchip system fabricated with MEMS technology was developed to detect bioelectrical signals. The developed microchip using the conductivity of gold nanoparticles could detect the biopotential with a high sensitivity. For designing the microchip, simulations were performed to understand the effects of the size and number of nanoparticles, and the sensing width between electrodes on the detection of biosignals. Then, a series of experiment was performed to validate the simulation results and understand the feasibility of the proposed microchip design. Both simulation and experimental results showed that as the sensing width between electrodes increased the conductivity decreased. Also, the conductivity increased as the density of gold nanoparticles increased. In addition, it was found that the conductivity that changes with the nanoparticles density could be approximated by a cumulative normal distribution function. The developed microchip system could effectively apply when a biosignals should be measured with a high sensitivity.

대학 피부미용 전공에서 TBL 수업 개발 연구

박정연(Jeong-Yeon Park),심현애(Hyeon-Ae Sim) 한국엔터테인먼트산업학회 2018 한국엔터테인먼트산업학회 학술대회 논문집 Vol.2018 No.11

오감 인터랙션을 활용한 문화유산 디지로그 전시 기법 연구

박정연(Jeong-Yeon Park),유정민(Jeong-Min Yu) 한국컴퓨터정보학회 2020 한국컴퓨터정보학회 학술발표논문집 Vol.28 No.2

정신장애인의 지역사회 복귀를 위한 의사결정 지원 -공법적 분석을 중심으로-

박정연(Park, Jeong Yeon) 숭실대학교 법학연구소 2022 法學論叢 Vol.53 No.-

최근 정신장애인에 대한 보건복지정책에서는 자기결정 존중과 지역사회 복귀가 중요한 목표이자 가치로 자리잡고 있다. 정신장애인의 지역사회 복귀는 비자의입원 정신장애인의 탈시설 문제를 포함하는바, 최근 비자의입원 정신장애인의 의사결정 지원을 위한 절차보조사업이 진행되고 있다. 이 글은 현행 절차보조서비스가 정신장애인의 지역사회 복귀를 위한 의사결정 지원제도로서 확대·발전하기 위해서는 충분한 공법적 정당화 논리를 갖출 필요가 있다는 인식에서 출발하였으며, 의사결정 지원에 관한 입법 과정에서부터 그 공법상 원칙과 헌법적 정당성에 대해 숙고하도록 하고자 의도하였다. 이를 위하여, 먼저 정신장애인의 지역사회 복귀의 함의와 의사결정 지원의 필요성에 대한 검토와 함께 정신장애인에 대한 의사결정 지원을 목적으로 하는 절차보조서비스를 살펴보았다. 나아가, 비자의입원 정신장애인에 대한 절차적 보장을 ⅰ) ‘처분’에 대한 행정절차 보장, ⅱ) 신체구속에 따른 조력권 보장 및 ⅲ) 자기결정권에 근거한 의사결정 지원 보장이라는 세 관점으로 구분하였는바, 특히 ⅲ) 자기결정권에 근거한 의사결정 지원이라는 관점이 헌법재판소의 결정례나 행정법규에서 충분히 반영되지 못하였음을 지적하였다. 한편, 정신장애인의 지역사회 복귀를 위한 의사결정 지원의 법적 실현을 위하여 먼저 절차보조서비스의 제도적 확대 가능성과 그 방향을 논하였으며, 자기결정권의 적극적 권리성에 주목하여 적어도 비자의입원에서의 입원·퇴원·의료적 결정 및 지역사회 복귀 후 외래치료의 영역에서는 국가에 대하여 구체적인 의사결정 지원 입법과 조치를 요구할 수 있는 헌법상 권리가 있음을 도출하였다. 마지막으로, 보호자에 의한 의사결정 지원과 공적 지원 간의 관계를 살핌으로써 정신장애인의 지역사회 복귀에 있어서 공적 의사결정 지원의 주체 문제를 검토하였다. In recent health and welfare policies for the mentally disabled, respecting self-determination and return to community are becoming important goals and values. Return of the mentally disabled to community inevitably includes the problem of de-facility of the mentally disabled, and a procedural assistance project is recently underway to support the decision-making of the mentally disabled. This article started with the recognition that the current procedural assistance service needs to have sufficient public legal justification logic to expand and develop it as a decision support system for the mentally disabled to return to community, and intended to contribute to the consideration of public law principles and constitutional justification. First, I reviewed the implications of returning to the community and the necessity of decision-making support for the mentally disabled, as well as the procedure assistance service aimed at supporting decision-making for the mentally disabled. Furthermore, I distinguished procedural guarantees for compulsory hospitalization for the mentally disabled people into three perspectives: (i) administrative procedures for disposition, (ii) assistance in physical detention, and (ⅲ) decision-making support, and pointed out that the view of decision-making support based on the right to self-determination was not sufficiently reflected in the decisions of the Constitutional Court or administrative laws. Finally, I discussed the possibility and direction of the systematic expansion of procedure assistance services for the return of mentally disabled people to the community, and drew up constitutional rights to require specific legislation and measures regarding to decision-making support in compulsory hospitalization, medical decision and outpatient treatment. And by examining the relationship between decision-making support by guardians and public support, I discussed how public decision-making support should be made in the return of mentally disabled people to the community.