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기계적 인장 자극에 의한 마이크로패턴화된 기질에서의 세포 배열
박수아,김인애,이용재,황영미,김동화,서승석,신정욱 한국생체재료학회 2006 생체재료학회지 Vol.10 No.2
Mechanical stimuli, such as pressure, tensile or biaxial stretching, fluid-induced shear stress, have been applied to cells to examine the responses of the cells. Also, cells elongate in the direction of the groove and migrate guided by the grooves. Cells are subjected to compression, tension, and shear in the body and undergo specific biochemical changes to respond and adapt to deformation. Cell deformation can be induced by mechanical forces such as cyclic stretch, fluid shear stress, and hydrostatic compressive pressure. Especially, fibroblasts in anterior cruciate ligament (ACL) are continuously stimulated from stretching environment and are aligned along the collagen fibers. This suggests that the mechanical forces change cellular responses such as morphological changes, protein synthesis, cell proliferation, and gene expression. In this study, human ligament fibroblasts (HLFs) were cultivated on micropatterned silicone substrates subjected to cyclic stretch, simulating ligament motion. The profile of silicone substrate was 10/50 and 20/50 μm in width (groove/ridge) and 3 μm in depth. Strain was applied over two days for 4 hours per day with a frequency of 0.5 Hz and its magnitude was set to be 8%. The purpose of this study was to evaluate ligament fibroblast alignment and cellular responses according to pattern size of microgrooved surface and stretching. Ligament fibroblasts in the microgrooved surface were elongated and aligned parallel to the microgrooves under no stretch. However, the formation of stress fibers and their orientation tend to be aligned perpendicular to the stretching direction. And, nuclei position on groove or ridge of micropatterned silicone substrate were located mainly on groove (>=62.5%). We suggest that the silicone microgrooves can be a useful tool to study the ligament fibroblasts under stretch. These results confirmed that for the regeneration of tissue in relation to tissue engineering the isolated and cultured cells or tissues should be cultivated under both appropriate biomechanical and morphological environments. However, further study is recommended to analyze the mechanism of the various cellular responses due to the mechanical stretching to evaluate the optimal mechanical condition for the control of the cellular responses in relation to tissue engineering.
영지약침과 왕뜸 복합치료의 복부비만 개선사례: 증례보고
박수아,이흔주,백지영,손경우,임경태 한방비만학회 2016 한방비만학회지 Vol.16 No.1
This study was conducted to investigate the effects of Ganoderma lucidum pharmacopuncture and moxibustion on abdominal obesity. Five women with abdominal obesity were treated 6 times in 2 weeks with Ganoderma lucidum pharmacopuncture on abdominal fat followed by 30 minutes of moxibustion on abdominal area. Before treatment and after 6 treatments were finished, body weight, body mass index, body fat mass, waist circumference, hip circumference and waist-hip ratio were measured. After all treatments, all average measured values were reduced. Body weight and body mass index were reduced by 0.34 kg and 0.46 kg/m2 respectively. Also, waist circumference and waist-hip ratio were reduced by 4.52 cm and 0.04 respectively. We suppose combined therapy of Ganoderma lucidum pharmacopuncture and moxibustion might be a time-effective treatment for abdominal obesity.
흰쥐에서 Chloramphenicol에 의해 유도된 불면에 대한 Amitriptyline의 효과
박수아,한진희,김정수,이성필 대한신경정신의학회 1999 신경정신의학 Vol.38 No.3
연구목적: 저자들은 흰쥐에서 항생제인 chloramphenicol을 투여한 후 수면의 변화 양상을 알아보고, 삼환계 항우울 제의 하나인 amitriptyline를 투여하여 항생제에 의해 유도된 불면증에 대한 항우울제의 효과를 조사하고자 하였다. 방 법: 실험동물로는 체중 270∼300g의 Sprague-Dawley 수컷 흰쥐 11마리를 사용하였고, 실험군(5마리)과 대조 군(6마리)으로 나누고 뇌파 측정 장치를 부착시킨 후 3일간(baseline, 1일, 2일) 연속적으로 수면-각성활동을 관찰하였다. 투약 1일째 실험군과 대조군에게 chloramphenicol 150mg/kg을 복강내 주사하였다. 투약 2일 째 대조군에게 chloramphenicol 150mg/kg을 복강내 주사하였고, 실험군에게는 chloramphenicol 150mg/kg 과 amitriptyline 12mg/kg을 복강내 주사하였다. 결 과: 1) 항생제 투여 후 야간 서파수면과 총 REM 수면이 유의하게 감소하였다. 통계적으로 유의하지는 않았지 만 light period 동안 REM 수면이 더 감소하는 경향을 보였다. 2) 항우울제 투여 후 실험군은 대조군에 비하여 야간 서파수면과 총 서파수면이 증가되었으며, 야간 각성과 총 각성이 유의하게 감소하였다. REM 수면이 light period 첫 3시간 동안 유의하게 감소하였고, dark period 후반부에는 오히려 baseline보다 증가하였다. 결 론: 이상의 결과로서 항생제에 의해 유도된 불면이 항우울제 의해 향상됨을 확인할 수 있었다. 본 연구의 실험 에 사용된 방법은 불면증의 동물모델로서 향후 항우울제의 수면효과를 규명하고 나아가 항생제를 사용중인 불면증 환자와 우울증 환자의 치료에 도움이 될 수 있을 것으로 생각된다 Objectives:Several studies have shown that administration of antibiotics decreased sleep amount as well as bacterial colony numbers in rats. It is well known that antidepressants decrease rapid eye movement( REM) sleep and increase slow wave sleep(SWS) in both human and animal studies. However, little is known to date about the effects of antidepressant on antibiotic induced insomnia in rats. Therefore the authors studied the effects of amitriptyline on chloramphenicol induced insomnia in rats. Methods:The Sprague-Dawley strain rats were divided into control(N=6) and experimental group (N=5). The sleep-wake activity was recorded continuously for consecutive three days(baseline, day 1 and day 2). On day 1 both groups were intraperitoneally injected with chloramphenicol 150mg/kg. On day 2 the control group was intraperitoneally injected with chloramphenicol 150mg/kg only, whereas experimental group was injected with amitriptyline 12mg/kg and chloramphenicol 150mg/kg. Results: 1) Chloramphenicol administration decreased dark period SWS and total REM sleep. 2) Amitriptyline administration increased total SWS and dark period SWS as well as decreased total wakefulness and dark period wakefulness. There was a decrease of REM sleep in the first 3 hours of light period, whereas a tendency of increase of REM sleep around the end of dark period. Conclusion:From the above results, it is concluded that amitriptyline may be effective to reverse chloramphenicol induced insomnia in rats. Furthermore, chloramphenicol induced insomnia in rat could be a useful model for insomnia studies in the future.