돼지의 체외수정

박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.1

The present study was conducted to investigate the optimum conditions of in vitro fertilization by ejaculated boar spermatiozoa capacitated in a defined medium. When fresh ejaculated boar spermatozoa were preincubated for 4h with elevated sperm concentration fertilization rates were markedly increased. The fertilization rates by spermatozoa preincubated at the concentrations of 2, 4, 10, 20 and 40×10^8 cells/ml were 12, 30, 50, 76 and 82%, respectively, However, when spermatozoa preserved for 10-12h at 20℃ were preincubated for 4h with elevated sperm concentration fertilization rates were markedly decreased. The fertilization rates by spermatozoa preincubated at the concentrations of 2, 4, 10, 20 and 40×10^8 cells/ml at preincubation were 91, 77, 68, 44 and 8%, respectively. When oocytes matured in vitro were inseminated with spermatozoa at the concentration 1 × I0^6 cells/ml, rates of fertilization and polyspermy were 97 and 87%, respectively, after culture for 20h without changing medium. When oocytes were inseminated with spermatozoa at the different concentrations of 1, 0.5, 0.25 and 0.1×10^6 cells /ml and the medium was changed 5h after insemination, the fertilization rates were 75, 73, 61 and 30%, respectively. The rates of polyspermic fertilization were 58, 47, 40 and 47%, respectively, when oocytes were inseminated with different concentrations of spermatozoa, and there was no difference in the rates of polyspermy fertilization among groups with changing medium. The present results suggested that both sperm preincubation with high concentration and sperm preservation at 20℃ for 10-12h were effective for capacitation ejaculated boar spermatozoa and that a complete block of polyspermy was not achieved in this in vitro fertilization conditions.

학술발표회 : 번식 ( 포스터발표 ) ; HOST ( Hypo - Osmotic Swelling Test ) 에 의한 동결융해 소정자의 평가

박수봉,정범식,연성흠,이명식,성환후,박용윤 한국동물자원과학회 ( 구 한국축산학회 ) 1995 한국축산학회 심포지움자료집 Vol.- No.-

퍼지 논리를 적용한 트롤 어구의 3차원 제어

박수봉,이춘우,이지훈 한국수산해양기술학회(구 한국어업기술학회) 2018 한국수산해양기술학회 학술발표대회 Vol.2018 No.04

학술발표회 : 번식 ( 포스터발표 ) ; Swim - up 후 회수된 소정자를 이용한 새로운 HOST 기술 개발

박수봉,정범식,장원경,전익수,정진관 한국동물자원과학회 ( 구 한국축산학회 ) 1995 한국축산학회 심포지움자료집 Vol.- No.-

생분해 어구 보급 확대에 따른 경제적 효과 분석

박수봉,김현영,양용수,어승섭,강다영 한국수산해양기술학회 2019 수산해양기술연구 Vol.55 No.4

최근 전 세계적으로 해양 쓰레기, 해양 플라스틱 문제가 심각해짐에 따라 이를 해결하기 위한 다양한 방안이 제시되고 있으며, 생분해 어구 사용이 하나의 대안으로 제시되고 있다. 현재 13종의 생분해 어구가 보급되고 있으나 보급률은 8.7%에 불과하다. 하지만 해양생태계 보호를 하고자 하는 인식이 점차 확대됨에 따라 생분해 어구 사용에 대한 필요성이 점차 커지고 있다. 따라서 본 연구에서는 우리나라 연근해 자망 및 통발에서 생분해 어구 보급이 확대됨에 따른 경제적 효과를 해양생태계 보전, 어획효율성 증대, 수산자원 증대 효과를 통해 분석하였다. 분석 결과, 전체 자망 및 통발에서 생분해 어구 보급이 10%, 20%, 30%로 확대됨에 따라 45,932백만원, 105,591백만원, 158,386백만원의 경제적 효과가 발생하는 것으로 나타났다. Recently, as the global problem of marine debris and marine plastics is getting serious, various ways to solve them have been proposed, and the use of the biodegradable fishing gear is proposed as an alternative. Currently, 13 types of biodegradable fishing gears are available, but their penetration rate is only 8.7%. However, the need for the use of the biodegradable fishing gear is growing as awareness of protecting marine ecosystems is growing. Therefore, in this study, the economic effects of the expansion of biodegradable fishing gear distribution in Korea's coastal areas were analyzed through the conservation of marine ecosystems, augmentation of fisheries resources, and augmentation of fishing efficiency. According to the analysis, the economic effects of 52,795 million won, 105,590 million won, and 158,385 million won was generated as the distribution of biodegradable fishing gear were expanded to 10%, 20% and 30% across all gill net and trap.

눈의 굴절이상도와 각막난시도에 관한 연구

박수봉 김천대학교 1994 김천대학교 논문집 Vol.15 No.-

The total astigmatism and corneal astigmatism of the ametropic eyes (aged 22-28, 69 eyes of 69 males and 81 eyes of 81 females, total 150 eyes) were measured by the subjective refraction and keratometry, respectively. The residual astigmatism were estimated from the total astigmatism and corneal astigmatism. The results were as follows: The amount of the eye's total astigmatism by measuring the subjective refraction was from 0 to 6.00D (mean 1.35D). The total astigmatism was with-the-rule for 110 eyes (84%) of the patients, against-the-rule for 12 eyes (9.5%), and oblique for 4 eyes (3.2%). The amount of the corneal astigmatism (or keratometric astigmatism) was from 0.11D to 3.52D (mean 1. 19D). The corneal astigmatism was with-the-rule for 124 eyes (82.7%) of the patients, against-the-rule for 9 eyes (6%), and oblique for 17 eyes (11.3%). It was fo-unded that most of the eye's astigmatism due to the corneal toricity. The mean amount of the residual astigmatism was 0.62D. Except for 21 cyes (under 0.12D of the amount), the residual astigmatism was with-the-rule for 46 eyes (35.7%) of the patients, against-the-rule for 67 eyes (51.9%), and oblique for 16 eyes (12.4%). The total and corneal astigmatism was mainly with-the-rule (84% and 82.7%, respectively), wh-ereas 51.9% of the residual astigmatism was against-the-rule.

부자가정에서 음주문제로 발생한 아동학대 사례개입

박수봉 어린이재단 (구 한국복지재단) 2006 사회복지관 사례연구 Vol.18 No.-

소난포란의 체외성숙과 유전공학적 응용

박수봉 中央大學校 遺傳工學硏究所 1989 遺傳工學硏究論集 Vol.2 No.1

The development of system for maturation of follicular oocyte in vitro is necessary to acquire competent oocyte and early embryos in plentiful number and at a price for studies of the many new technologies. Bovine follicular oocytes matured in vilro achieved fertilization and sudsequently cleavage, but the frequencies of both preimplantation development to blastocyst stage and live young after transfer were low compared to the oocytes matured in vivo. Hence, it has been concluded that a deficient cytoplasmic maturation occures in spontaneously maturing oocytes even though nuclear maturation appear nomal. Probably, the abnormality of spontaneously matured oocytes may have been the result of deficiency in culture system. Also, this problem may be due to unhealthy oocytes recovered from ovaries at random stages of the estrus cycle. This seems likely since hitological studies show that the majority of vesicular follicles on bovine ovaries are in some stages of atresia. These factors indicate that the studies for maturation of bovine follicular oocyte in vilro need improvement of culture conditions and to define the characteristics that might be indicative of healthy oocyte.

CIE X_(10) Y_(10) Z_(10)색표시계에 따른 선글라스용 렌즈의 측색

박수봉,마기중 金泉大學 1992 논문집 Vol.11 No.1

시감투과율이 82%이상으로 한국규격에서 선글라스용 렌즈로 구분될 수 없는 23개 렌즈를 제외한 22개 렌즈에 대해서 CIE X_(10) Y_(10) Z_(10)색표시계에 따른 주파장과 자극순도를 조사한 결과는 다음과 같다. 1. 각기 다른 염료로 착색된 렌즈의 주파장은 각각 갈색 : 582-596nm(CIE A). 575-590nm(CIE B). 572-587nm(CIE D_(65)), 핑크색 : 592-595nm(CIE A). 589-592nm(CIE B). 588-591nm(CIE D_(65)), 청색 : 481-485nm(CIE A). 475-479nm(CIE B). 474-477nm(CIE D_(65), 녹색 : 556-564nm(CIE A). 550-556nm(CIE B). 547-553nm(CIE D_(65))이었다. 2. 자극순도가 25% 이상으로 KS규격을 초과한 렌즈의 염료별 착색시간은 각각 갈색 :30초 이상 (CIE A & D_(65)) 1분이상 (CIE B), 핑크색 : 2분 이상 (CIE A), 5분이상 (CIE D65 & 8), 청색 : 10분 이상 (CIE D_(65)), 20분 이상(CIE B), 녹색 : 20분 이상(CIE A, D_(65) & B)이었다. 한편 평균주광 (D_(65)) 의 color shifts 에 대한 ANSI규격을 초과한 렌즈는 5분 이상 갈색 염료로 착색된 렌즈와 20분 동안 착색된 핑크색 렌즈뿐이었다. The transmittance properties of 45 dyed CR-39 lenses were examined to determine whether these lenses met the KS P-4404 Lenses for Sunglass standards for excitation purity, and colorimetered according to the CIE 1964 supplementary standard colorimetric system. Approximately 50% of the sampled dyed lenses failed to meet KS requirement for luminous transmittance. Of 22 lenses that passed to KS requirement for luminous transmittance. only 11 lenses that met KS requirement for exitation purity.

고능력 젖소 번식 효율 개선방안

박수봉,Park, Su-Bong 한국종축개량협회 2006 種畜改良 Vol.11 No.12