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자외선-B에의한 Hairless mouse의 보호기능과 표면구조의 변화 영향
김진준 ( J. J. Kim ),박문억 ( M. E. Park ),강세훈 ( S. H. Kang ) 대한화장품학회 1992 대한화장품학회지 Vol.18 No.1
Hairless mouse에 자외선-B를 일회 조사한 후 시간에 따른 피부보호기능과 표면구조변화의 정도를 TEWL과 주름살밀도로 측정하였다. 자외선-B의 조사 량은 0.5, 1과 3 MED 였으며, 측정 및 관찰은 조사전과 조사 후 1, 3, 5, 7 및 14일에 수행하였다. TEWL은 Evaporimiter로, 주름살밀도는 silicone replica와 image-analyzer를 이용하여 측정하였다. 조사전과 0.5MED 그룹들의 TEWL값은 변화가 없었으나, 1과 3MED그룹들의 TEWL값은 현저한 변화를 보여 3일째에 극대 값을 보였고, 이후 감소하기 시작하여 14일째에는 거의 정상 값에 도달하였다. 반면 주름살밀도는 자외선-B의 조사량에 비례하는 경향을 보여 모든 조사 그룹에서 3-5일째까지 감소한 후 서서히 증가하였다. 결과로부터, 1) 자외선 조사 후 시간에 따른 TEWL과 주름살밀도의 최대, 소 값은 3일째에 나타났으며, 2) 주름살밀도의 회복정도가 조사량에 상관없이 시간에 따라 일정한 기울기를 보였으며, 3) 주름살밀도측정만이 Sub-erythemal 손상의 정도차를 유의하게 보여주었다. Hairless mice (Skh:HR-1) exposed to single doses (0.5, 1.0 and 3. OMED) of UV-B radiation were displayed remarkable changes of barrier function and surface morphology. Trans- epidermal water loss (TEWL) as an index of barrier function was measured by evaporimeter, and wrinkle density (WD) as an index of morphological alteration was measured by image analyzer. Significant changes of TEWL were not observed in the control and 0. SMED group, but 1.OMED and 3. OMED groups noted significant difference. TEWL of 3. OMED group was rapidly increased to the 3rd day and decreased until the 14th day when it reached nearly to normal level, Time-courses of TBWL for 1. OMEB and 3. OMED groups displayed similar pattern, but different only in the magnitude. WD were significantly decreased during the 3rd-5th day in all of the irradiated groups and then increased during the last period to the 14th day, but did not recover the normal level at the 14th day. Time-courses of WD for all groups exhibited similarity, and were entirely dependent on the exposed doses. We also observed histological changes which included hyperplasia, sunburn cell (SBC) formation, accumulation of polymorphonuclear leukocyte (PMNs), and loss of collagen of UVB- exposed hairless mouse skin. Changes of TEWL and WD are helpful in understanding of epidermal and dermal damages by single exposure of UVB
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朴文億,梁弘鉉,白永基 全北大學校 附設 畜産開發硏究所 1991 畜産開發硏究報告 Vol.4 No.-
This study was undertaken to obtain basic data on the fetal endochondral ossification in the native Korean cattle scapula, using histochemical observation to determine the earliest stages of both chondrification and ossification. Thirty scapulae, from a series of embryos and fetuses of the korean native cattle ranging from 11 to 70mm in crown-rump (C-R) length were used The ossification center was observed in the supra and infraspinus fossa in the 5th group (CRL 51-60mm), markedly ossified in the 6th group (CRL 61-70mm) by Alizatin red S stain. The chondrogenic center was observed in the 1st group (CRL 11-20mm), and the primary ossification center was presented in the 4th group (CRL 41-50mm) by H-E stain. Osteocytes, Osteoblasts, Myeloid cells, and irregular ladder shaped trabeculae were observed in actively developing areas of endochondral ossification in the 5th group (CRL 51-60mm)by H-E stain. Alcianophility was increased markedly in the intererritorial matrix in the 3rd group (CRL 31-40mm). Also this reaction was decreased markedly in the interterritorial matrix, the adjacent portion to the marrow cavity, and trabecula in the 5th group (CRL 51-60mm).
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