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사염화탄소 처리한 SD(rat)에 대한 진균발효쌍화탕의 간독성 치료효과 연구
이재훈(Lee Jaehoon),마충제(Ma Choongje),하혜경(Ha Hyekyung),전원경(Jeon Wonkyung),박화용(Park Hwayong),마진열(Ma Jinyeul) 한국한의학연구원 2008 한국한의학연구원논문집 Vol.14 No.1
In this research, as a method for verifying the efficacy of Ssangwhatang and fungus-fermentated Ssangwhatang, a comparative study on the liver protection effect was conducted using animal experiments by inducing the liver toxicity with the CG₁₄ treatment. Inducing the liver damage resulted in the increase in the serum AST and ALP activity, and one day administration of the test material(CCl₄: 0.5 ㎖/㎏/day) caused 520 IU/L of the ASP activity leading to 29% enhancement in comparison with the normal group and 93% and 81% reductions in the fungus-fermentated Ssangwhatang-administered groups, BFST1 and BFST2, respectively. ALT is 42 IU/L for the normal group and 99 IU/L for the negative control group leading to 135% enhancement. 15 ㎖/㎏/day and 30 ㎖/㎏/day administrations of fungus-fermented Ssangwhatang(BFST) resulted in 51% and 45% decreases in the ALT concentration, respectively. One day administration of 30 ㎖/㎏ Ssangwhatang and fungus-fermentated Ssangwhatang caused the LDH in the plasma to tend to decrease. CCl₄(1.0 ㎖/㎏/day) administered at the 0th and 4th days led to the observation of the tendency toward the decrease in AST, ALT, and LDH contents. The results indicate that the function of Ssangwhatang is partly reinforced under the fungus-fermentated Ssangwhatang performed in order to verify the efficacy of Ssangwhatang's effect on the recovery from fatigues.
엄영란(Youngran Um),이지혜(Jihye Lee),마진열(Jinyeul Ma) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.1
The purpose of this study was investigation of quantitative analysis of marker substances in solid fermented Angelicae Gigantis Radix by High performance liquid chromatography(HPLC). HPLC was performed for determination of nodakenin and decursin in solid fermented Angelicae Gigantis Radix extract, the separation method was performed on C18 column (250 ㎜ × 4.6 ㎜, 5 ㎛, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (330 nm). The flow rate was 1.0 ㎖/min. Retention time of nodakenin and decursin was about 11.47, 46.79 min and linearity of calibration was showed good result(r2=0.9999, 0.9999), respectively. Content of nodakenin was 0.76 ± 0.02% in control, 0.31 ± 0.00% in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), 0.51 ± 0.02% in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), 0.82 ± 0.03% in Angelicae Gigantis Radix extract fermented with honey(SST)(p<0.05) and 0.88 ± 0.01% in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.01). Content of decursin was 4.50 ± 0.08% in control, 2.90 ± 0.05% in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), 2.65 ± 0.08% in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), 4.46 ± 0.11% in Angelicae Gigantis Radix extract fermented with honey(SST) and 4.73 ± 0.04% in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.05), respectively.
이지혜(Jihye Lee),이재훈(Jaehoon Lee),마충제(Choongje Ma),마진열(Jinyeul Ma) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.1
In this study, we investigated the single dose toxicity and safety about water-extracted Galgeuntang. To evaluate single dose toxicity, 20 male and 20 female ICR mice were observed survival rates, general toxicity, changes of body weight for 14 days after single oral administration of 0 (control group), 1250, 2500 and 5000 ㎎/㎏ Galgeuntang. And after 14 day, We observed autopsy. Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 ㎎/㎏). LD50 of Galgeuntang might be over 5000 ㎎/㎏ and it is very safe to ICR mice.
박화용(Hwayong Park),이지혜(Jihye Lee),조장원(Changwon Cho),마진열(Jinyeul Ma) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.3
In this research, the acute toxicity of fermented Yukmijihwangtang extract was examined using male and female ICR mice, To evaluate the acute toxity and safety, 0(control group), 1250, 2500 and 5000 ㎎/㎏ of fermented Yukmijihwangtang extract were orally administered to male and female ICR mice. After single administration, we observed survival rates, general toxicity, changes of body weight for the 14 days and autopsy at 1 day following the administration according to the Regulation of Korean Food and Drug Administration. Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 ㎎/㎏). LD?? of fermented Yukmijihwangtang extract might be over 5000 ㎎/㎏ and it is very safe to ICR mice.
Lactobacillus fermentum으로 발효한 쌍화탕의 파골 세포 분화와 난소 적출한 랫트의 골다공증에 미치는 영향
심기석(Kishuk Shim),이지혜(Jihye Lee),이재훈(Jaehoon Lee),마진열(Jinyeul Ma) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.1
Objective : Ssangwha-tang is a traditional medicine formula widely prescribed for a decrease of fatigue after an illness in Korea. The aim of this study is to investigate the effect of Ssangwha-tang fermented by Lactobacillus fermentum (SF) on osteoclast differentiation in vitro and on bone metabolism of an ovariectomized rat in vivo. Methods : Tartrate-resistant acid phosphatase activity and staining were applied to evaluate the formation of osteoclasts. Ovariectomized rats were orally administrated with SF (30 ㎖/㎏/day) for 12 weeks. Serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total cholesterol, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, triglyceride, phosphate, calcium levels were determined. Effect of SF on bone loss were studied by histological analysis and the measurement of bone mineral density. Results : SF significantly inhibited tartrate-resistant acid phosphatase activity and formation of osteoclasts in RAW264.7 cells stimulated by receptor activator for nuclear factor κB (NF-κB) ligand (RANKL). In addition, SF significantly decreased the level of triglyceride and increased the level of low-density lipoprotein. Moreover, the decrease of trabeculae of the femur was partially prevented in ovariectomized rats administrated with SF. Conclusions : SF treatment could prevent ovariectomy induced bone loss and its effects could be medicated by the inhibition of osteoclastogenesis.
엄영란(Youngran Um),심기석(Kishuk Shim),이재훈(Jaehoon Lee),박화용(Hwayong Park),마진열(Jinyeul Ma) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.1
The aim of this study was to study the quantitative analysis of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica, Ganoderma lucidum, honey or Nuruk. The amounts of dry on loss were measured and the quantitative analysis of glycyrrhizic acid was performed by high performance liquid chromatographic (HPLC). HPLC method was performed on C18 column (250 ㎜ × 4.6 ㎜, 5 ㎛, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (254 ㎚). The flow rate was 1.0 ㎖/min. Retention time of glycyrrhizic acid was about 23.96 min and linearity of calibration was R²=0.9998. Contents of glycyrrhizic acid in Glycyrrhizae Radix extract (control) was 5.048 ± 0.14; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica (SDT) was 1.975 ± 0.07; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Ganoderma lucidum (SYT) was 2.676 ± 0.07; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with honey (SST) was 5.191 ± 0.06; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Nuruk (SNT) was 5.305 ± 0.34, respectively. Contents of glycyrrhizic acid in SDT and SYT were decreased but that in SST and SNT was increased when compared to control.
이지혜(Jihye Lee),엄영란(Youngran Um),박화용(Hwayong Park),이재훈(Jaehoon Lee),마진열(Jinyeul Ma) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.2
The purpose of this study was investigation of quantitative analysis of marker substances in Paeonia lactiflora extracts by solid fermentation. High performance liquid chromatography (HPLC) for the determination of albiflorin and paeoniflorin in P. lactiflora extracts by solid fermentation, the separation method was performed on C18 column (250 ㎜ × 4.6 ㎜, 5 ㎛, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (230㎚). The flow rate was 1.0 ㎖/min. Retention time of albiflorin and paeoniflorin was about 28.88, 31.92 min and linearity of calibration was showed good result(r2 = 0.9998, 0.9996), respectively. Content of albiflorin was 0.090 ± 0.03% in P. lactiflora extract(control), 0.102 ± 0.00% in P. lactiflora extract fermented with Paecilomyces japonica, 0.056 ± 0.01% in P. lactiflora extract fermented with Ganoderma lucidum, 0.093 ± 0.00% in P. lactiflora extract fermented with honey and 0.046 ± 0.00% in P. lactiflora extract fermented with Nuruk. Content of paeoniflorin was 4.506 ± 0.13% in control, 2.599 ± 0.04% in P. lactiflora extract fermented with Paecilomyces japonica, 1.222 ± 0.03% in P. lactiflora extract fermented with Ganoderma lucidum, 2.750 ± 0.05% in P. lactiflora extract fermented with honey and 0.847 ± 0.00% in P. lactiflora extract fermented with Nuruk, respectively. Content of the marker substances did not increase in all fermentation experiment group.
영지버섯 균사체를 이용한 고체 발효 쌍화탕의 급성독성에 관한 연구
엄영란(Youngran Um),박화용(Hwayong Park),이재훈(Jaehoon Lee),심기석(Kisuck Shim),마진열(Jinyeul Ma) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.1
This study was carried out to investigate the acute toxicity and safety of Ssanghwa-tang extract fermented with Ganoderma lucidum. To evaluate the acute toxity and safety, 0(control group), 1250, 2500 and 5000 ㎎/㎏ of Ssanghwa-tang and fermented Ssanghwa-tang extracts were orally administered to 20 male and 20 female ICR mice. After single administration, we observed survival rates, general toxicity, changes of body weight, and autopsy. Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 ㎎/㎏). LD?? of Ssanghwa-tang and fermented Ssanghwa-tang extracts might be over 5000 ㎎/㎏ and it is very safe to ICR mice.
쌍화탕과 발효쌍화탕 분획물의 폴리페놀함량 및 항산화 활성
김동선(Dongseon Kim),엄영란(Young-ran Um),양민철(Minchul Yang),윤나영(Nayoung Yun),마진열(Jinyeul Ma) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.3
The aim of this study is to compare antioxidant activity and total polyphenol contents between before and after fermentation of Ssanghwa-tang according to solvent partition. Ssanghwa-tang was fermented with Lactobacilus fermentum. Ssanghwa-tang and the fermented Ssanghwa-tang were fractioned by solvent partition with ethyl acetate, butanol and water. The Ssanghwa-tangs and their solvent fractions were evaluated for total polyphenol contents and DPPH radical scavenging activity. The antioxidant activity as well as the total polyphenol contents were highest in each ethyl acetate fraction and significantly (p<0.05) increased after fermentation.