메이크업 시 사용되는 퍼프의 사용기간에 따른 오염도에 관한 연구

류재기 ; 김은주 ; 장철수 김천대학교 2010 김천대학교 논문집 Vol.31 No.-

The purposes of this study are to investigate the level of bacteriological contamination of puffs which are used most frequently for touch ups during the day as a makeup tool, and to compare the degree of contamination before and after washing puffs with a neutral detergent. A total of 40 powder puffs that were used for six months to three years have been collected since May, 2005. The collected puff samples were cut into the size of lem by a sterilized scissor, and were put into a sterilized test tube that contained 5ml of DW (distilled water). Then the tube was strongly agitated. Supernatants (100ml) con-taining bacteria was applied to TSA (Trypticase Soy Agar), MacConkey agar, and SDA (Sabouraud Dextrose Agar) plates, respectively. The TSA (Trypticase Soy Agar) and MacConkey agar plates were incubated at 37C for 24-48 hours. The SDA (Sabouraud Dextrose Agar) plates were incubated at 28 C for 7-14 days. As a result of the level of contamination of puffs used over a certain period of time, there was no significant difference cxcept aerobic bacteria. Staphylococcus was not detected. Aerobic bacteria was detected from the puffs older than one year and the colony count increased sharply. The puffs used for more than two years had three times more colony count than the one-year old puffs. In particular, out of 10 puffs older than two years, two were found with 500CFU/ml exceeding the limit of microbiology of cos-metics. Also, the puffs older than two years had 31,1% less microbiology after washing with a PH neutral detergent than before washing. Therefore, as the period of using puffs is longer, thorough checks of their hygiene are needed; as the number of using times increase or the length of using times is longer, frequent washing is inevitable. Since this study restrained the number of puffs to 40,7 would be problematic for generalization and a secondary experiment would be necessary for precise identification of cultured germs.

면역검사실에서 이월오염에 대한 영향의 분석

류재기 ; 장철수 김천대학교 2008 김천대학교 논문집 Vol.29 No.-

The purpose of the carry-over recovery experiment in clinical immunology test is performed to estimate effect variation from high concentration to low concentration. In practice, carry over experiment is a problem only for analyte with a wide clinical range of analyte concentration, such that a minute degree of carry over could have significant clinical implications. One suggested method to study carry-over contamination is to run known high level patient samples, followed by known low level patient samples to see if the results of the low level material are affected significantly. If carry-over is detected, th team leader and laboratory physician in managing it the analyte concentration above which subsequent patient samples may be affected, and define this output value in the procedure. Carry-over experiment should be expressed as a percentage because the experimental objective is to estimate to reduce effect variation from high level patient sample to low level patient sample, which is a percentage type of error, Good carry-over is 0%. The results of carry-over experiment were Imunoglobulin G (-0.41%), Imunoglobulin A (-0.86%), third complement component (-0.47%) , fourth complement (0.19%) with turbidimetric immunoassay of DADE Behring . hepatitis B surface antibody (0%), hepatitis B surface antigen (-0.004%), hepatitis B core antibody imunoglobulin M (0.12%), hepatitis B core antibody (0%), hepatitis B envelope antigen (0.0043%), hepatitis B envelope antibody (0.012%), with chemiluminescent microparticle immunoassay (CMIA) of ABBOTT Diagnostic Division in Germany. We concluded that the results of carry-over experiment compared to the range of error allowable for the test. There were no item betond the 1% criterion for acceptable performance.

한국인에서 혈청 중성지방, 복부비만과 허리둘레의 상관성연구

장철수 ; 류재기 ; 박갑태 김천대학교 2015 김천대학교 논문집 Vol.36 No.-

Purpose: The purpose of this study was to determine the association between serum triglyceride, abdominal obesity, and waist circumference in Korean. Method: A total of 168 healthy examinees that enrolled in this study were examined at Gimcheon Medical Center from July 2012 to December 2013. Waist circumference (WC) and body mass index (BMI) of subjects were measured as well as blood pressure, fasting blood sugar level, High Density Lipoprotein (HDL-cholesterol), Low Density Lipoprotein (LDL-cholesterol), cholesterol, and C-reactive protein (CRP). Results: In men, WC(p<0.011) had a significant correlation with triglyceride and BMI(p<0.001) had a significant correlation with triglyceride. In women(p<0.001), WC and BMI had a significant correlation with triglyceride. In men, triglycerides (r=0.501, p<0.011) had a positive correlation with WC. However, CRP (r=0.407, p<0.001) had a positive correlation with BMI. In women, triglycerides (r=0.470, p<0.001) and CRP (r=0.367, p<0.001) had a positive correlation with WC. Conclusion: Therefore, WC and triglyceride may be the most effective anthropometric indices for screening Korean people in regards to obesity.

겨우살이 lectin의 흰쥐 간세포 독성에 미치는 영향

장철수 ; 류재기 ; 김태호 김천대학교 2007 김천대학교 논문집 Vol.28 No.-

In the present study, we investigated to the cytotoxocity of Viscum album lectin in cultured rat hepatocytes. The cytotoxcity effect in Viscum album lectin was determined by the activity of LDH and the staining of hepatocyte. Viscum album lectin significantly increased LDH, which leakage into medium, of hepatocytes treated or untreated with CCI₄ (P<0.001). However, Viscum album lectin signifi-cantly increased LDH leakage from CC1₄-induced hepatocyte (P<0.001). There was a significant increase in LDH levels relative to the control group. Histological observation basically supportedly the result obtained from LDH assay. The livers of rats challenged with CC1₄ produced a marked increased cyto- plasmic vacuoles and inflammatorycells, while the number of necrotic cells and swollen hepatocytes was not changed significnatly. Rats administered DMSO alone did not alter the normal hepatic architecture. Histological observation of liver sec- tion in rat treated 72 h with either Viscum album lectin CCI₄-induced liver damage showed number of cytoplasmic vaculoe and necrotic cell. The number of inflam- matory cell increased markedly. This results suggest to the conclusion that Viscum album lectin has the effect of hepatotoxicity activator.

대학생들의 콘텍트렌즈 저장용기의 미생물 오염도 조사

류재기, 장철수, 김현경 김천대학교 2013 김천대학교 논문집 Vol.33 No.-

The use of contact lenses is currently increasing because of a various consumers requirements such as those related to treatment and cosmetic issues. The increase in the number of contact lens users has been accompanied by eye infection due to improper han-dling of contact lenses. Therefore, this study was performed to determine the contamina-tion levels of lens storage cases, which can be an important cause of contamination of con-tact lenses, the cases belonged to students from the Department of Biomedical LaboratoryScience, Gimcheon University. A total of 62 lens storage cases were used in this study, and our results showed that 63% (39 cases) were contaminated with microorganisms. Stenotrophomonas maltophilia, Serratia marcescens, Achromobacter denitrificans, and fungi were detected in 8%, 10%, 72%, and 10%, respectively, of the cases. Stenotrophomonas maltophilia is a non-pathogenic bacterium; however, it is resistant to several antibiotics and is thought to cause endocarditis and meningitis. Eye infection with Serratia marcescens can lead to microbial keratitis and contact lens-induced acute red eye, particularly during overnight contact lens wear. Fungal eye infection is characterized by eye redness, pain, excessive tears formation and blurred vision. In conclusion, the results, which showed that a high proportion of the contact lens storage cases studied were contaminated with bacteria and fungi, suggest that providing proper education regarding the use and storage of contact lenses is important to prevent contact lens-induced eye infection.

요로감염의 원인균과 항생제에 대한 고찰

조기창 ; 류재기 ; 장철수 김천대학교 2007 김천대학교 논문집 Vol.28 No.-

In order to provide useful information on the choice of adequate drugs in the treatment of urinary tract infections (UTI), this paper analyzed the causative organisms of urinary tract infection and studied their changes in antibiotic sensi- tivity from the year of 2000 to 2004 for five years. 5,452 uropathogens and their antimicrobial sensitivity were analyzed from 4,815 inpatients and outpatients, all of whom had causative organisms more than 105 CFU/ml in their urine cultures. As a result of the study, the incidence of UTI increased.with age and was highest in patients who were in their 70s (21.3%). The incidence of Gram negative organ- isms that were measured through Gram stain decreased from 74.2% in 2000 to 58.7% in 2004, whereas Gram positive organisms increased from 25.8% in 2000 to 41.3% in 2004. The major Gram negative and positive pathogens were Escherichia coli (29.0%), Enterococcus (15.9%), Klebsiella (9.4%), and Escherichia coli was the most importanat pathogen of UTI during the research period. In terms of a test conducted on antibiotic sensitivity to Gram negative organisms, the susceptibility of amikacin, an aminoglycoside, increased from 78.2% in 2000 to 82.3% in 2004, and that of trimethoprim-sulfamethoxazole decreased from 66.2% in 2001 to 60.6% in 2004. As for antibiotic sensitivity to Gram positive organisms, the susceptibility of clindamycin increased from 46.2% in 2000 to 68.2% in 2004. However, the antibiotic susceptibility of trimethoprim-sulfamethoxazolen decreased from 90.7% in 2001 to 76.8% in 2004. As for Escherichia coli, the main causal microorganisms of urinary tract infection, the antibiotic sensitivity of cefa- zolin decreased from 88.3% in 2002 to 83.0% in 2004; as for Klebsiella, the sen- sitivity of amikacin decreased from 93.1% in 2000 to73.7% in 2004; and as for the trimethoprim-sulfamethoxazole, the antibiotic sensitivity of Staphylococcus aureus decreased from 100% in 2001 to 86.7% in 2004. In conclusion, the study confirms that it is possible to use cephalosporins and aminoglycosides as initial experiential antibiotics for urinary tract infection, and suggests that trimethoprim-sulfamethoxazole, traditionally used as primary treat- ment for urinary tract infection, should be used more carefully to treat urinary tract infection accurately.

혈액배양에서 분리된 Extended Spectrum β-Lactamase 생성균의 역학적 특성 조사

서충원 ; 류재기 ; 장철수 김천대학교 2008 김천대학교 논문집 Vol.29 No.-

the data which analyze the results of blood cultures are crucial basic information of the empirical therapy for patients with infection since the patterns of the species of microorganism isolated from blood and the results of antibiotics resistance of in-patients as well as out-patients in one hospital in the middle area of South Korea from 2004 to 2006. Blood culture was performed with BacT/Alert 3D(North Carolina, Durham, USA) for 5 days using BacT/Alert SA (Areobic) and BacT/Alert SN (Aneorbic) medium for adult patient and BacT/Alert PF medium for children patients. The antibiotic susceptibility test was performed with VITEK (BioMerieux vitek. Hazelwood. Missori. USA) automated identification as well as susceptibility equipments. Total 39,305 cases of blood culture sample were investigated and positive patients of 2,216 (20.0%) were found. Among those, there were 40 patient with ESBL positive, and blood culture positive smples were 4,798 (12.2%). ESBL positive bacteria were found in 86 samples (including double checked culture bacteria). The majority of ESBL producing bacteria were E. coli, K. pneumoniae and K. oxitoca as ordering based on the number. The research showed the results that there were monre females than male with the bacterias, more E. coli in over 50 years old aged group than other bacterias and largest number of patients with 13 patient (32.5%) in Chungcheongnam – do province were found. he most common ESBL producing bacteria were E. coli throughout 3 years, but K. pneumoniae and K. ocitoca were also fairly found. Interestingly, E. coli was highly found in over 50 years old patients. In conclusion, recovery progress of patients will be badly affected by the high emergence rate of ESBL producing bacteria due to the using of broad spectrum antibiotics. Therefore, significant considering is necessary in the antibiotic use, and continuous infection control and trace observations for all lpatients in hospitals including infected patients are required.

겨우살이 lectin의 흰쥐 간세포 독성에 미치는 영향

장철수 ( Choul Soo Chang ),류재기 ( Jae Ki Ryu ),김태호 ( Tae Ho Kim ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3

In the present study, the author investigated to the cytotoxocity in cultured rat hepatocytes of Viscum album lectin. The cytotoxcity effect in Viscum album lectin on the activity of LDH was also investigated. Viscum album lectin significantly increased LDH leakage into medium of hepatocytes treated or untreated with CCl4 (p<0.001). However, Viscum album lectin significantly increased LDH leakage from CCl4-induced hepatocyte (p<0.001). There was a significant increase in LDH levels relative to the control group. Histological observation basically supported the result obtained from LDH assay. The livers of rats challenged with CCl4 produced a marked increased cytoplasmic vacuoles and inflammatory cells in number, while the number of necrotic cells and swollen hepatocytes did not change significnatly. Rats administered DMSO alone did not alter the normal hepatic architecture. Histological observation of liver section in rat treated 72 hrs with either Viscum album lectin CCl4-induced liver damage showed number of cytoplasmic vaculoe and necrotic cell. The number of inflammatory cell increased markedly. This results suggest to the conclusion that Viscum album lectin has a effect of hepatotoxicity activator.

임상병리과 학생을 대상으로 한 임상 실습에 관한 연구

류재기 ( Jae-ki Rye ),장철수 ( Choul-soo Chang ) 대한임상검사과학회 2010 대한임상검사과학회지(KJCLS) Vol.42 No.2

The aim of this study was to investigate the problem areas in the current clinical training of biomedical laboratory science education which may guide the development of an effective strategy for managing clinical training for biomedical laboratory science students. A self-reporting questionnaire was developed by the researchers. This survey was conducted between May 1st and May 15st in 2009 and 92 questionnaires werecollected from senior biomedical laboratory science students. In Analyzing data, chi-square test was used for categorical variables. 82.6% of respondents was satisfied with conditions of the training institutes. 30~40% of the respondents reported that they were not satisfied with the programs and supervision systems for training students even though the staff members of other disciplines were supportive in general. Although83.7% of the respondents reported that they were obtaining information and knowledge for their professional carrier through the training, 70.7% of the respondents reported that they had negative experiences during the training. According to the result of the survey, it can be inferred that the structure of the training education needs to be more systematically organized in order to train the well prepared entry-level biomedical laboratory scientists.

겨우살이 Lectin이 흰쥐의 혈청지질 및 간조직에 미치는 영향

장철수(Chul-Soo Jang),류재기(Jae-Ki Ryu) 한국콘텐츠학회 2012 한국콘텐츠학회논문지 Vol.12 No.9

본 연구에서 국내산 겨우살이 lectin이 흰쥐 간의 지방형성에 미치는 영향을 알아보기 위해 혈청 내 지질함량을 측정하였고, 이를 확인하고자 간조직의 지방형성 변화를 조사하였다. 렉틴의 분리는 sephadex G-150로 여과 및 sepharose 4B affinity chromatography 로 정제하였다. CCl₄를 복강 내에 투여시 72시간 경과 후 혈청 총cholesterol 및 triglyceride는 정상군 및 olive oil 투여군에 비해 유의하게 증가하였으며, lectin과 CCl₄를 동시 투여 하였을 때는 유의한 감소를 나타내였다. 흰쥐 간의 조직학적 관찰은 CCl₄투여군은 정상군 및 olive oil 투여군에 비해 지방변화 및 간세포 내에 많은 대소의 cytoplasmic vacuole가 보였으나, CCl₄와 lectin을 동시 투여시는 CCl₄ 투여군에 비해 cytoplasmic vacuole은 현저히 감소하였고, 이에 비례하여 혈청 cholesterol 및 triglyceride 측정치가 현저히 감소하는 결과를 나타내었다. 이상의 결과를 볼때 흰쥐에서 lectin이 혈청 지질을 감소시키거나 또는 조직학적 관찰에서 간조직의 지질형성을 억제시키는 효과가 있는 것으로 확인되었다. This study was carried out to investigate effect of serum lipid and liver tissue in lectin from Viscum album on rats. The lectin was purified by sepharose 4B affinity chromatography and gel filtration using sephadex G-150 with plant material from Viscum album. After 72 h of CCl₄ injection, there was a significant increase in serum total cholesterol and triglycerige levels relative to the control group. However, treatment of both Viscum album and purified lectin were significantly decreased lipid parameters against the CCl₄-induced. Histological observation of the liver section of rats challenged with CCl₄ produced a marked increase of cytoplasmic vacuoles in number, while rats administered olive oil alone did not alter the normal hepatic architecture. Histological observation of the liver section in rats treated 72 h with either Viscum album purified lectin or CCl₄-induced liver lipogenesis showed decreased numbers of cytoplasmic vaculoes and necrotic cells. These results suggest that Viscum album lectin has a significantly decreased lipid in serum or histological observation of the liver section decreased in lipogenesis in rats.