Automated Nucleic Acid Extraction Systems for Detecting Cytomegalovirus and Epstein-Barr Virus Using Real-Time PCR: A Comparison Study Between the QIAsymphony RGQ and QIAcube Systems

김한아,허미나,김지영,문희원,윤여민,조현찬 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.2

Background: Cytomegalovirus (CMV) and Epstein-Barr virus (EBV) are increasingly important in immunocompromised patients. Nucleic acid extraction methods could affect the results of viral nucleic acid amplification tests. We compared two automated nucleic acid extraction systems for detecting CMV and EBV using real-time PCR assays. Methods: One hundred and fifty-three whole blood (WB) samples were tested for CMV detection, and 117 WB samples were tested for EBV detection. Viral nucleic acid was extracted in parallel by using QIAsymphony RGQ and QIAcube (Qiagen GmbH, Germany), and real-time PCR assays for CMV and EBV were performed with a Rotor-Gene Q real-time PCR cycler (Qiagen). Detection rates for CMV and EBV were compared, and agreements between the two systems were analyzed. Results: The detection rate of CMV and EBV differed significantly between the QIAsymphony RGQ and QIAcube systems (CMV, 59.5% [91/153] vs 43.8% [67/153], P=0.0005; EBV, 59.0% [69/117] vs 42.7% [50/117], P=0.0008). The two systems showed moderate agreement for CMV and EBV detection (kappa=0.43 and 0.52, respectively). QIAsymphony RGQ showed a negligible correlation with QIAcube for quantitative EBV detection. QIAcube exhibited EBV PCR inhibition in 23.9% (28/117) of samples. Conclusions: Automated nucleic acid extraction systems have different performances and significantly affect the detection of viral pathogens. The QIAsymphony RGQ system appears to be superior to the QIAcube system for detecting CMV and EBV. A suitable sample preparation system should be considered for optimized nucleic acid amplification in clinical laboratories.

웹 드라마 광고 음악의 극적 기능 고찰 : 이마트 웹 드라마 광고를 중심으로

김한아 한국엔터테인먼트산업학회 2018 한국엔터테인먼트산업학회논문지 Vol.12 No.8

This paper discussed the dramatic functions of advertisement music of Web Drama. We examined this after analyzing the advertisement music of web drama that combines elements of advertisement and drama among brand contents. Ad-music in the form of web drama should show a longer and four steps in composition of music, unlike just short advertisement music. It is necessary to expose repetitive music that feels different while being the same by re-harmonizing basic motifs to naturally recognize the brand. Examining the causality studies of exposure and preference, there is a research result 'repeating an object provides a basis for memory and immersion to the recipients eventually. Through this, it was concluded that repeated exposure of advertising music of web drama can make the recipient aware of the brand only by music in the end. The dramatic function of web drama advertisement music is to expose basic motifs repeatedly and to give quick brand recognition to the recipient through various reharmonization and to experience more dramatic ads at the climax.

Multilocus Sequence Typing for Candida albicans Isolates from Candidemic Streptococcus suis Causes Septic Arthritis and Bacteremia: Phenotypic Characterization and Molecular Confirmation

김한아,이상훈,문희원,김지영,이선화,허미나,윤여민 대한진단검사의학회 2011 Annals of Laboratory Medicine Vol.31 No.2

Streptococcus suis is a swine pathogen that causes meningitis, septicemia, pneumonia, and endocarditis. The first case of human S. suis infection was reported in Denmark in 1968, and since then, this infection with has been reported in many countries, especially in Southeast Asia because of the high density of pigs in this region. We report the case of a patient with septic arthritis and bacteremia caused by S. suis. Cases in which S. suis is isolated from the joint fluid are very rare, and to the best of our knowledge, this is first case report of S. suis infection in Korea. The identity of this organism was confirmed by phenotypic characterization and 16S rRNA sequence analysis. An 81-yr-old Korean woman who presented with fever, arthralgia, and headache was admitted to a secondary referral center in Korea. Culture of aspirated joint fluid and blood samples showed the growth of S. suis biotype II, which was identified by the Vitek2 GPI and API 20 Strep systems (bioMérieux, USA), and this organism was susceptible to penicillin G and vancomycin. The 16S rRNA sequences of the blood culture isolates showed 99% homology with those of S. suis subsp. suis, which are reported in GenBank. The patient’s fever subsided, and blood and joint cultures were negative for bacterial growth after antibiotic therapy; however, the swelling and pain in her left knee joint persisted. She plans to undergo total knee replacement.

비사실적 연기술 - 연극 ‘두드리 두드리’ 공연사례를 중심으로 -

김한아 한국연기예술학회 2018 연기예술연구 Vol.12 No.2

공연 양식에 따라 배우가 적용하는 연기술에는 분명한 차이가 있다. 하지만 직접 그 차이를 경험하지 않고서는 어느 부분의 적용으로 어떤 문제가 일어나는지 알기 힘들 뿐더러 배우가 연기를 하면서나 연출 이 방향을 제시 하면서, 교육자가 연기교육을 하면서 때때로 ‘명확한’ 문제점을 파악하거나 문제점에 대 한 ‘정확한 방법’을 제시하는 것이 쉽지만은 않다. 또한, 연극에 있어서 ‘실기’는 하나의 학문적 연구 작 업으로서 충분히 알고 작품에 임해야 하나 실 현장은 훈련이나 테크닉에 관해 직접 오랜 시간 실습해보 고 실험을 한 경우는 많지 않다. 때문에 새로운 작품은 넘쳐나고 여러 예술가들이 만들어 놓은 양식 속 에서 연기하는 배우로서는 이러한 방대한 각 작품들에 자신에 ‘꼭 맞는 표현’을 찾는데 어려움을 겪어왔 으며 그러한 부분을 학문적으로 연구를 하기에 쉽지 않다. 필자는 실제 공연을 하는 연기 현장 속에서 실기와 이론을 나누지 않고 연기실습의 주가 되고 있는 사실주의 테크닉을 비롯한 비사실적 테크닉의 내 면과 실 활용의 의미와 한계, 연기활용의 발전 가능성을 바라보려한다. 이 연구는 공연이 이루어지기 위해 준비하는 과정에서 공연 양식에 따른 배우의 연기테크닉 활용에 대한 연구이다. 그중에서도 비사실주의 연극에서의 표현방법을 찾아가는 과정을 필자가 직접 참여하여 했었던 연기훈련, 신체훈련, 공연일지, 동선표 등으로 기록하여 분석 및 연구한 논문이다. 필자가 연기한 연극 ‘두드리 두드리’를 통하여 연기 현장에서 실기와 이론의 내면을 들여다봄으로서 그 내용과 의미를 연구하였고, 특정 양식과 역할 극대화를 위해 적용했던 테크닉들의 효과와 한계를 알아보는데 목적을 두 었다. 이 연구에 나와 있는 테크닉 적용을 통한 효과와 제기된 문제를 통하여 좀 더 다양하고 깊은 연기 테크닉의 개발과 작품에 참여하는 연출과 배우의 테크닉활용, 연기테크닉 교육에 도움이 되었으면 한다. 또한 한국연극의 좀 더 창조적인 작업을 도모할 수 있는 발판이 되길 바란다. Depends on the performance style, there are obvious differences how actors apply their dramatics. It is hard to recognize which problem will be caused by applying certain part before experiencing that difference directly. Not only that it is hard to identify a well-defined problem or suggest a correct procedure when actor plays, director present how to act, or teacher provide a lesson. Furthermore, practice in acting has to be faced as an intellectual research work whereas it is hard to practice or experience training or technique for a sufficient time in the real theater field. Finding a suitable expression to each one of work or researching that particular part academically is difficult as an actor who plays in a certain forms which had created from various artist. The author would like to introduce realistic technique which is major part of acting practice for both practice and theory in real performance field as well as inside of unrealistic technique, meaning and limitation of actual application and development possibility of acting utilization. This research is intended to find application of dramatic technique that actor has to prepare to act depends on performance style. Most of all, it is focused on how to find expression manner in unrealistic technique from research and analysis of acting training, physical training, performance log, and pathway chart that author has participated. Research had made by looking in the inside of practice and theory from acting filed of ‘Doodri Doodri’, performance that author had performed and it is intended to see effect and limitation of technique applied for particular method and role maximizing. With effect from application of technique and posed problem described in this research, author wants to contribute development in various and inspired dramatic technique as well as technique application, acting technique education of directors and actors who participate in the play work. Moreover, it will be grateful if it becomes a foothold to promote creative work in Korean theater.

혈액원 진단혈액학 검사의 정도관리

김한아,허미나 대한수혈학회 2017 大韓輸血學會誌 Vol.28 No.1

The primary goal in transfusion medicine is to promote high standards of quality in all aspects of transfusion, including production, management, and transfusion of blood components. To achieve this goal, a transfusion service quality management system should be established. Such a system should include several organizational structures, responsibilities, policies, processes, procedures, and resources with provided specifications and regulations. In this review, we summarize the current knowledge and practices regarding the quality management of hematology tests applied to the donor selection and production of blood components, including red blood cells, platelets, fresh frozen plasma, and cryoprecipitates in the field at blood centers.

중등학교 통합환경 경도장애학생의효과적 학교상담을 위한 협력의 촉진요인 탐색

김한아,오인수 이화여자대학교 특수교육연구소 2020 특수교육 Vol.19 No.1

Purpose: The purpose of this study was to foster collaboration among the key stakeholders(special educators, general educators, and school counselors) to improve the school counseling services for students with mild disabilities in inclusive middle and high school. Method: A research method called “concept mapping” was used. First, 26 key stakeholders brainstormed idea statements of facilitating factors of collaboration. Subsequently, 41 key stakeholders sorted and rated the list of statements. Finally, the researcher computed the final concept map based on multidimensional scale analysis and hierarchical cluster analysis. Results: The multidimensional scale analysis identified two dimensions: ‘system-subject’ and relationship-expertise.’ A total of 7 clusters were derived through hierarchical cluster analysis: “school structure and administrative perception improving inclusion,” “physical environment and human resources in school improving disability awareness,” “school atmosphere fostering collaboration,” “positive relationship and intimacy among the key stakeholders,” “active communication for problem-solving among the key stakeholders,” “general educators’ non-discriminatory instructions and special educators’ intervention for disability awareness,” and “the key stakeholders’ expertise on counseling intervention for students with disabilities.” Conclusion: Based on the rated importance, the researcher discussed the perception of the key stakeholders and differences among them. 연구목적: 본 연구는 중등학교 통합환경의 경도장애학생을 위한 학교상담에서 핵심 주체인 특수교사, 담임교사, 학교상담자가 인식한 협력의 촉진요인을 탐색하여 장애학생의 학교상담 활성화를 위한 방향을 제안하고자 하였다. 연구방법: 개념도 연구방법을 적용하여 먼저 특수교사, 담임교사, 학교상담자 26명의 개별 면담을 통한 아이디어 진술문으로 구성된 질적 자료를 수집하였다. 이후 특수교사, 담임교사, 학교상담자 41명이 이 자료의 유사성을 기준으로 분류하고 중요도를 평정한 후 다차원척도 분석과 위계적 군집분석을 실시하였다. 연구결과: ‘체제-주체’, ‘관계-전문성’의 2차원에 바탕을 둔 개념도가 도출되었다. 개념도상에는 총 7개 군집이 드러났는데, 이는 진술문 간 유사성이 높은 순으로 ‘장애학생 친화적 체제 및 학교관리자의 인식’, ‘장애 인식 개선을 돕는 학교 내 인적, 물적 환경’, ‘각 주체 간 협력을 촉진하는 분위기’, ‘각 주체 간 긍정적 관계 및 친밀감’, ‘각 주체 간 문제해결을 위한 적극적 소통’, ‘담임교사의 비차별적 지도 및 특수교사의 장애 인식 개선을 위한 개입’, ‘각 주체의 장애학생을 위한 상담적 개입 전문성’이다. 결론: 중요도 평정 결과를 기반으로 협력의 촉진요인에 대한 전체 집단의 인식과 각 집단 간 인식의 차이를 논하고 연구의 의의와 제한점, 후속연구를 위한 제언을 제시하였다.

Variant Burkitt-type translocation (8;22)(q24;q11) in plasma cell myeloma

김한아,문희원,윤여민,Chul-Min Park,Mark Hong Lee,허미나 대한혈액학회 2011 Blood Research Vol.46 No.2

Variant Burkitt-type translocation, t(8;22)(q24;q11), is very rare in plasma cell myeloma. We report a 51-year-old male patient with plasma cell myeloma, who showed t(8;22) (q24;q11). He suffered from pelvic pain for two months, and showed IgG, lambda type of monoclonal gammopathy (5.14 g/dL; 49.9% of protein). His bone marrow examination showed increased plasma cells (66.9% of all nucleated cells). Plasma cells (74.9% of all nucleated cells) and monoclonal spike (3.38 g/dL; 42.2%) persisted after three cycles of thalidomide and dexamethasone. Cytogenetic analysis showed complex chromosomal abnormalities: 44,XY,-1,t(2;5)(q33;q13),add(8)(q24.1),t(8;22)(q24.1;q11.2),add(10) (p15), der(11)t(1;11)(q21;p11.2),del(12)(p11.2p13),-13,-14,add(14)(q32),der(15)t(1;15)(p22;p11.2),-16,add(17)(q11.2),+21,+1-3mar[cp6]/46,XY[19]. To the best of our knowledge, this is the first report on plasma cell myeloma with a variant Burkitt-type t(8;22)(q24;q11) in the Korean patient. A review of 11 such cases in the literature, including the present case, implicated that plasma cell myeloma with t(8;22)(q24;q11) might be related to advanced stage and poor prognosis.

젊은 나이에 발생한 렘수면행동장애와 정량적 분석을 통한 진단적 접근

김한아,한수현,이상암 대한수면연구학회 2015 Journal of sleep medicine Vol.12 No.1

Rapid eye movement sleep behavior disorder (RBD) is a sleep disorder characterized by loss of muscle atonia during REM sleep associated with dream enactment, which usually start at the age of older than 50. RBD in elders are known to be developing symptoms of neurodegenerative disorders in the course of disease. However, the pathophysiology and prognosis of “early-onset” idiopathic RBD is unclear. Several existing standard value about REM sleep without atonia (RSWA) of RBD patients was established with “late-onset” RBD for those diagnosed at age 50 and greater, RSWA metrics in “early-onset” RBD patients diagnosed prior to age 50 years have not been previously described. Therefore, we report here two patients who were diagnosed as “early-onset” idiopathic RBD, and performed quantitative RSWA scoring. We suggested that “early-onset” idiopathic RBD has different etiologies the various characteristics of RSWA than adults with RBD.

Proenkephalin, Neutrophil Gelatinase-Associated Lipocalin, and Estimated Glomerular Filtration Rates in Patients With Sepsis

김한아,허미나,이성호,Rossella Marino,Laura Magrini,Patrizia Cardelli,Joachim Struck,Andreas Bergmann,Oliver Hartmann,Salvatore Di Somma 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.5

Background: Proenkephalin (PENK) has been suggested as a novel biomarker for kidney function. We investigated the diagnostic and prognostic utility of plasma PENK in comparison with neutrophil gelatinase-associated lipocalin (NGAL) and estimated glomerular filtration rates (eGFR) in septic patients. Methods: A total of 167 septic patients were enrolled: 99 with sepsis, 37 with septic shock, and 31 with suspected sepsis. PENK and NGAL concentrations were measured and GFR was estimated by using the isotope dilution mass spectrometry traceable-Modification of Diet in Renal Disease (MDRD) Study and three Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equations: CKD-EPICr, CDK-EPICysC, and CKD-EPICr-CysC. The PENK, NGAL, and eGFR results were compared according to sepsis severity, presence or absence of acute kidney injury (AKI), and clinical outcomes. Results: The PENK, NGAL, and eGFR results were significantly associated with sepsis severity and differed significantly between patients with and without AKI only in the sepsis group (all P<0.05). PENK was superior to NGAL in predicting AKI (P=0.022) and renal replacement therapy (RRT) (P=0.0085). Regardless of the variable GFR category by the different eGFR equations, PENK showed constant and significant associations with all eGFR equations. Unlike NGAL, PENK was not influenced by inflammation and predicted the 30-day mortality. Conclusions: PENK is a highly sensitive and objective biomarker of AKI and RRT and is useful for prognosis prediction in septic patients. With its diagnostic robustness and predictive power for survival, PENK constitutes a promising biomarker in critical care settings including sepsis.

Circulating Biologically Active Adrenomedullin Predicts Organ Failure and Mortality in Sepsis

김한아,허미나,Joachim Struck,Andreas Bergmann,Salvatore Di Somma 대한진단검사의학회 2019 Annals of Laboratory Medicine Vol.39 No.5

Background: Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection. Biologically active adrenomedullin (bio-ADM) is an emerging biomarker for sepsis. We explored whether bio-ADM concentration could predict severity, organ failure, and 30-day mortality in septic patients. Methods: In 215 septic patients (109 patients with sepsis; 106 patients with septic shock), bio-ADM concentration was measured at diagnosis of sepsis, using sphingotest bio-ADM (Sphingotec GmbH, Hennigsdorf, Germany) and analyzed in terms of sepsis severity, vasopressor use, and 30-day mortality. The number of organ failures, sequential (sepsis-related) organ failure assessment (SOFA) score, and 30-day mortality were compared according to bio-ADM quartiles. Results: Bio-ADM concentration was significantly higher in patients with septic shock, vasopressor use, and non-survivors than in patients with solitary sepsis, no vasopressor use, and survivors, respectively (all P<0.0001). Bio-ADM quartiles were associated with the number of organ failures (P<0.0001), as well as SOFA cardiovascular, renal, coagulation, and liver subscores (all P<0.05). The 30-day mortality rate showed a stepwise increase in each bio-ADM quartile (all P<0.0001). Bio-ADM concentration and SOFA score equally predicted the 30-day mortality (area under the curve: 0.827 vs 0.830). Conclusions: Bio-ADM could serve as a useful and objective biomarker to predict severity, organ failure, and 30-day mortality in septic patients.