Chitosan 부분가수분해물과 PALA 와의 가교체의 담즙산염 흡착성에 관한 연구

김천호,최규석 ( Chun Ho Kim,Kyu Suk Choi ) 한국공업화학회 1995 공업화학 Vol.6 No.5

천연 고분자인 chitin을 탈아세틸화 시킨 chitosan은 자체의 아민기가 답즙산과 흡착능을 가져 혈중 콜레스테롤 값을 저하시킴이 보고 되고 있다. 이 chitosan의 용해성을 높이기 위해 먼저 cellulase (Triohoderma reesei)로 가수분해하여 chitosan 부분 가수분해물의 혼합물인 Fraction A, B 그리고 C를 제조하여 이 중 저당의 혼합물인 Fraction C와 polyallyamine을 glutaraldehyde를 이용하여 표면 가교시켜 질소함량이 각각 9.87, 9.34, 8.10%인 Resin I, II, III을 합성하였다. 담즙산염인 cholic acid sodium salt와 deoxycholic acid sodium salt를 tris (hydroxymethyl) aminomethane buffer (pH 7.5) 용액에 각각 녹여, 새로이 제조한 각 수지를 가하여 농도에 따른 흡착량을 HPLC로 조사한 결과, 모두 chitosan 보다 우수한 흡착능을 나타내었고, chitosan 부분 가수분해물과 polyallyamine의 몰비를 1:1로 조정하여 제조한 가교체인 Resin II가 흡착량이 가장 높았다. Chitosan is the deacetylation product of chitin. Its binding with bile salt in intestine is reported to cause the lowering concentration of cholesterol in serum. In order to increase the bind with bile salt, chitosan was modified as follow. Hydrolyzed chitosan mixture were obtained by enzymatic hydrolysis with trichoderma reesei, and then Fraction C of them was crosslinked with polyallylamine (Mw=50,000) by glutaraldehyde. The weight percents of nitrogen of crosslinked polymers are 9.87% for Resin I, 9.34% for Resin II, 8.10% for Resin III. After cholic acid sodium salt and deoxycholic acid sodium salt were dissolved in tris (hydroxymethyl) aminomethane buffer (pH 7.5) respectively, Resin I, II, III were incubated in the solution for 1hr. As a result, Resin I, II, Ill were improved than chitosan itself. Especially Resin II exhibited the best binding result than other resin.

학교의 변화와 정보 통신 기술 활용 교육

김천호 한국스쿨넷학회 2003 학술발표 논문집 Vol.2003 No.1

개 난자의 체외성숙에 미치는 호르몬과 Na-Pyruvate의 영향

김천호 한국동물생명공학회(구 한국동물번식학회) 2006 Reproductive & developmental biology Vol.30 No.1

본 연구는 호르몬과 Na-pyruvate의 첨가가 개 미성숙난자의 체외성숙에 미치는 영향을 검토하였다. 개의 발정주기와 관계없이 암캐의 난소에서 회수한 난자를 NCSU-37 배양액에서 72시간 체외 배양하였다. 호르몬의 영향을 검토하기 위하여 배양액 중에 다양한 호르몬을 24{sim}72시간 첨가하여 성숙율을 검사하였으며, Na-pyruvate 첨가 농도의 영향을 검토하였다. E2 단독 첨가구에서는 제2감수분열 중기(MII) 단계까지 성숙이 관찰되었으나(5.7%), 타 처리구에서는 MII기까지의 성숙이 이루어지지 않았다(P<0.05), E2를 6~24시간만 처리하였을 때는 MII기로 성숙되는 난자가 없었으나 72시간 처리하였을 때는 6.0%로 유의적으로 높게 나타났다(P<0.05). Na-pyruvate의 농도를 0.25 mM 첨가한 구보다 2.5 mM 첨가한 구에서 제1감수분열 중기(MI) 단계까지의 성숙율이 증가하는 경향을 보였으나, MII 단계까지의 성숙율은 차이가 없었다. 본 실험의 결과는 개 난자의 체외성숙시 E2가 첨가된 배양액에서 72시간 배양시에 성숙율을 증진시킬 수 있으나, Na-pyruvate의 농도는 개 체외배양에 있어 큰 영향을 미치지 않는 것을 나타낸다. This study was conducted to examine the effects of hormone and sodium pyruvate on in vitro maturation of canine oocytes. Canine oocytes were collected from the ovaries of dogs and cultured in NCSU-37 medium with hormones and sodium pyruvate for 72 hr. Oocytes matured to the metaphase II (MII) stage were observed only from estradiol 17β (E2), and the presence of gonadotropin did not improve the nuclear maturation. No oocytes were developed to the MII stage when E2 was added to medium during the first 6 and 24 hrs of culture period. The presence of E2 during the whole culture period enhanced the nuclear maturation to the MII stage (6.0%, P<0.05). High concentration of sodium pyruvate (2.5 mM) slightly enhanced the nuclear maturation to the metapahse I (HMI) stage, but not the MII stage. the result of the present study shows that the presence of E2 during the whole culture period of 72 hr enhances the maturation of canine oocytes to the M stage, but sodium pyruvate does not affect the nuclear maturation of the canine oocytes.

한국과 일본인의 식행동에 관한 조사연구(제 2 호) : 식사담당참여와 식사예법에 관하여 On Participation in Cooking and Table Manners

김천호,방하문자 한국식생활문화학회 1989 韓國食生活文化學會誌 Vol.4 No.2

Of 1,244 junior high school students of boys and girls and their families in Tokyo and Seoul, we investigated the present situations of the participation in cooking and table manners. In both countries, the average age of parents was 40's, and the ratio of the kinds of fathers' job was similar including 75% of full-time salaried workers, while that of mothers' in Japan was 63%, in Korea 23%. The male participation in cooking in both countries was found in younger generations and that of fathers and boys in Korea was significantly fewer than in Japan, which is regarded as the influence of Confucianism and employment of housekeepers. The figure of frequency of supper taken together daily was 27% in Japan and 54% in Korea where they didn't begin eating until all families gathered or the elders began. In Japan the civilities before and after meals were so often customarily expressed and they had the regular order of seats. The figure of frequency of taking meals with TV watching was about 45% in Japan of breakfast and supper and more than 30% in Korea of supper. As for the participation in cooking and table manners, national characteristics were clearly found out. In both countries, the newly modernized and democratized style of dietary behaviors was being made, rather sooner in Japan, out of the specific East-Asian traditional dining culture.

무공해 자동차 개발의 현황과 전망

김천호 한국시멘트협회 1994 시멘트 Vol.136 No.-

유압서보 시스템을 위한 뉴로-퍼지 제어기 설계

김천호,조형석 대한기계학회 1993 대한기계학회논문집 Vol.17 No.1

본 연구에서는 제안된 뉴로-퍼지 제어기를 사용하여 유압 서보 시스템을 제어 하고 학습하기 위한 구조로써 유압 서보 시스템의 모델링을 위한 추가적인 노력이 필 요없는 feedback error learning 구조물 채택하였다. 학습 과정에서 필요한 유압 서 보 시스템의 입-출력 사이의 감도(sensitivity)의 효과는 학습 계수에 포함된다. 이 러한 형태의 제어기가 유압 서보 시스템 제어에 유용하게 적용될 수 있다는 것을 보이 기 위해서 불확실성과 높은 비선형성 뿐만아니라 외란의 영향을 받는 유압 서보 시스 템을 대상으로 시뮬레이션을 수행했다. 시뮬레이션 결과에 의하면 제안된 뉴로-퍼지 제어기는 수학적인 모델을 기초로한 기존의 제어 알고리즘에 비해 쉽게 구성할 수 있 고 높은 정밀도, 빠른 학습 속도를 얻을 수 있는 장점을 가지고 있음을 알 수 있다. Many processes such as machining, injection-moulding and metal-forming are usually operated by hydraulic servo-systems. The dynamic characteristics of these systems are complex and highly non-linear and are often subjected to the uncertain external disturbances associated with the processes. Consequently, the conventional approach to the controller design for these systems may not guarantee accurate tracking control performance. An effective neuro-fuzzy controller is proposed to realize an accurate hydraulic servo-system regardless of the uncertainties and the external disturbances. For this purpose, first, we develop a simplified fuzzy logic controller which have multidimensional and unsymmetric membership functions. Secondly, we develop a neural network which consists of the parameters of the fuzzy logic controller. It is show that the neural network has both learning capability and linguistic representation capability. The proposed controller was implemented on a hydraulic servo-system. Feedback error learning architecture is adopted which uses the feedback error directly without passing through the dynamics or inverse transfer function of the hydraulic servo-system to train the neuro-fuzzy controller. A series of simulations was performed for the position-tracking control of the system subjected to external disturbances. The results of simulations show that regardless of inherent non-linearities and disturbances, an accuracy tracking-control performance is obtained using the proposed neuro-fuzzy controller.

Mongol秘史의 飮食文化

김천호 한국몽골학회 2003 몽골학 Vol.0 No.15

인공피부의 연구개발 동향

김천호,손영숙 한국생체재료학회 2005 생체재료학회지 Vol.9 No.1

With the prolongation of average human life span and the overall aging of society, the demand for bio-artificial organs is increasing. From among artificial organs manufactured using tissue engineering technologies, artificial skin is expected to be introduced into the market first in Korea. With the overall and rapid aging of human society, the number of patients suffering from diabetic or decubitus ulcers, the greatest users of artificial skin, is expected to increase dramatically. At current technological levels, however, there exist several problems including high prices, difficulty of mass production, and short clinical testing periods. Coupled with high prices, the smallness of the domestic market itself is hindering market expansion. Despite tremendous progress in the artificial skin technology, problems including the possibility of side effects as well as the reduction of production costs, construction of a mass production system, and heightened public awareness of artificial skin must be resolved first for further market expansion.

忠北 地域의 社會科 敎育 地域化 方案

金天鎬 한국사회과교육연구회 1986 사회과교육 Vol.- No.19

학교교육과정의 이해와 편성·운영의 실제

김천호,조용남 淸州敎育大學校 初等敎育硏究所 1995 初等敎育硏究 Vol.6 No.-

Poet Sebastian in Suddenly Last Summer has a characteristic seeking after the configurations of gods. Sebastian is Adonis living a life under the protection of Aphrodite, and consanguineous-incest-like lover with Mrs. Venable led him to an abnormal relation with others. He found a picture of himself through the cruelty of gods detected in Encantadas Island. Sebastian, seeking some ways of atonement, let his body by torn by the boys who he had given maltreatment; which had some tint of the vicarious sacrifice of Christ. In Suddenly Last Summer, Sebastian in Christian myth is expressed to be related to Adonis in Greek myth. Although Sebastian, raised under his mother's overprotection, was absorbed in everything he tried, he was described as an individual of duplicity, a sexual pervert with internal conflict. This is a plot like a three-concerned relation with Aphrodite and Fersephone; and he, later captured by malice, made himself its sacrifice by transferring the concept of malice to that of gods. Mrs. Venable's trial to have Catherine undergo lobotomy is considered to be dismemberment, and Mrs. Venable's violent conciliation could be presumed to be an indication for the victory of Catherine's conscience to be granted. Mrs. Venable, Sebastian's mother, regarded her son's death such as in cannibalism, in which starving natives ate his flesh, as the martyrdom, figured the relation with her son as the life of the Goddess and Priestess, and took a metaphorical description on Attis-Cybele, plantation god, blessing the repetition of resuscitation and regeneration. Williams, through Christian and Greek myth, showed a roundabout description of the human's basic problems so called solitude, violence, justice, hypocrisy, and consciousness. He himself did not so much endeavor to find some clues of the problems the society at that time bore, as let his readers of audience find solutions themselves. Since Williams for this purposes took the mythological writing technique in his works, he ought to be estimated such a playwriter as made an unexhausted effort to set up the triangular positioned human nature escaping from decayed and corrupted materialism prevailing the society those days.