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金知和,裵炳胄 中央醫學社 1975 中央醫學 Vol.28 No.4
As Meyer and Mishell stated, histologically evidence of chronic nonbacterial endometritis persists for long period in endometrial specimen obtained from IUD users. It is generally accepted that this reaction in the endometrial cavity is main causative factor for the contraceptive effect of the IUD. Since the effect of all known carcinogenes in human is delayed, however, with a long patency period of about a decade, it will not be possible to make a definite statement on this point until substantial numbers of women have worn IUD's for a long period. At persent time, there is no evidence that IUD causes cancer in woman. Many long term studies have shown that there is no clinical evidence that the IUD causes an increased incidence of carcinoma of the endometrium and cervical carcinoma. Causative factors of uterine carcinoma, causative factors for the contraceptive effects of IUD, histopathologic changes in uterine specimens obtained from IUD users and some other problems retated to the effect of IUD are also discussed.
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牛脂 및 菜種油 添加 給食이 흰쥐의 營養에 미치는 影響
金知和 梨花女子大學校 韓國生活科學硏究院 1975 韓國生活科學硏究院 論叢 Vol.15 No.-
A study was carried out on the nutritional effects in rats for feeding diets containing 5% or 20% of fat, beef tallow or rape oil for 12 weeks. The experimental animals, 240 albino rats, a pure strain that have been kept with the stock diet for 2 weeks right after weaned were used, and their initial body weight for the experiment were about 80g. The animals were divided into 32 groups according to dietary condition and feeding periods. An any animal which showed unusual appearance during the study were excluded. The results obtained were summerized as follows: 1. The body weight of the experimental dietary group animals were generally lower than that of the control group throughout the study period. 2. The weight of organs, liver, kidney, spleen, and heart in the experimental dietary group animals have shown no difference from the control group up to 8 weeks of experimental period, but they have shown lower than that of the control group thereafter. 3. The hematocrit value and hemoglobin contents in the experimental dietary group animals have shown higher level than that of the control group up to 8 weeks, but they have shown no difference with the control group thereafter. 4. It has been no significant difference in nitrogen contents in the liver between the experimental period. 5. The total cholesterol contents in liver and serum, and free form cholesterol in the serum of the high rape oil dietary group animals have shown significantly high level than that of the control group throughout the experimental period. 6. The total cholesterol contents in the aorta of the high rape oil dietary group animals have shown higher level than that of the control group up to 8 weeks of the experimental period. It might be able to state through the above conclusion, the diet containing 10% fat has shown better nutritional effect on rats than either 5% or 20% fat diets. It is also implying that the high rape oil diet has some effects on animals growth and cholesterol metabolism in rats.
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쥐의 장 Ferritin의 정제와 성질에 관한 연구(硏究)
김지화,Kim, Chi-Wha 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.4
소장의 점막세포에 있어서 철 이온흡수에 관계되는 transport system의 존재에 대해서는 많은 이론이 있다. 본 연구에서는 철의 장관 흡수기구에 있어서 ferritin의 역할을 연구하기 위하여, 우선 장에 존재하는 ferritin의 정제를 시도 하였고, 그 성상에 대하여 몇가지 생화학적 검토를 하여 다음과 같은 결과를 얻었다. 1. 쥐점막세포에서 CM-cellulose chromatography를 사용하여 장 ferritin을 간단하고 용이하게 또 좋은 수율로 정제하는 방법을 확립하였다. 2. 정제하여 얻은 장 ferritin은 전기영동적, 면역학적으로 순수하고, 다른 단백질을 함유하고 있지 않았다. 3. 정제한 장 ferritin은 간 ferritin에 비하여, 단백질에 대한 철의 함량이 대단히 낮았고, 간 ferritin의 철 함량의 약 1/20이었다. 철 저장 단백질인 간 ferritin과는 기능적으로 다르다고 생각된다. 4. 장 ferritin과 등전초점 전기영동을 하면 간 ferritin과 같이 heterogeneity (iso-ferritin)가 관찰되었으며, 각기 pI (isoelectric point)의 분포범위는 간 ferritin의 것 보다도 산성측에 기울어져 있었다. 5. 장 feritin에 대한 방사성 철$(^{59}Fe)$의 취입은 간 ferritin의 경우 보다 낮은 값을 보였다. 6. 장 ferritin의 아미노산 조성은 간 ferritin과 비슷하나 methionine, isoleucine, proline에 있어서 약간 차이가 있었다. 7. 장 점막세포를 세포분획하고 각 획분 중의 장 ferritin의 존재를 검토한 결과, brush border 획분 supernatant 획분에 장 ferritin이 국한하여 존재하고, 다른 획분에는 존재하지 않았다. In the absence of an excretory pathway for excess iron, the adjustment of intestinal iron absorption to need is essential to the organism. No other nutrient is known to be regulated in this manner. There is a mucosal block to the absorption of iron, a gastrointestinal mechanism involving a special carrier, ferritin, regulates passage of iron from the intestinal lumen to plasma. In order to study the role of ferritin in the mechanism of intestinal absorption of iron, the isolation of ferritin and some chemical characteristics were investigated. The following results were obtained. 1. Mucosal ferritin was isolated from rat intestinal mucosa. 2. The precipitating test in gel revealed no serological difference between rat intestinal mucosa and liver ferritin. 3. The Fe/protein ratio of purified mucosal ferritin was strikingly lower than that of rat liver ferritin. 4. The isoelectric points of mucosal iso-ferritins were shifted to lower pH 4.80~5.10 than those of rat liver iso-ferritin (5.11~5.45). 5. The amino acid composition values were significantly different for liver and mucosal ferritin. 6. Mucosal ferritin was found in both the brush border fraction and the supernatant fraction of mucosal cellular components.
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불임피술주부(不妊被術主婦)의 가정적(家庭的) 배경(背景)에 관(關)한 연구(硏究)
김지화,Kim, Chi-Wha 대한생식의학회 1977 Clinical and Experimental Reproductive Medicine Vol.4 No.2
A study was carried out for the evaluation on family situation of 900 homemakers those who had received tubal sterilization operation (laparoscopic and minilaparotomy) at family planning clinic, Seoul Red Cross Hospital from October 1974 to September 1977. The results obtained are as follows: 1) In age distribution, predominant age group consisted of those $31{\sim}35$ years frequency of 43.2% and average age was 33.3 years. 2) Educational level showed that homemakers who finished primary school accounted for 37.3% of the total and those having middle school education comprised 28.7%, and 24.3% of them were high school graduates, 8.3% of them were college graduates. On the other hand, husband's education level showed that, 37.6% of them were high school graduates, 29.7% were college graduates and 19.9% were middle school graduates, therefor, educational level of husbands was one step higher than wives. 3) In the gravidity at the tubal sterilization, the highest incidence(18.4%) among 853cases was the group of gravida 5, and 16.2% gravida 4, and the gravidity ranged $1{\sim}23$. Average gravidity of clients was 6.0. 4) Among the total number of 900 clients, 778cases (91.3%) had no experience of spontanous abortion, history of 1 abortion in 5.9%, 2 abortions in 1.8%, and it showed the decresed incidence of spontanous abortion recently. Average was 0.15. As regarding induced abortion, in spite of only 142 homemakers (16.7%) had no history of induced abortion, 20.5% had experienced 1 induced abortion before sterilization, 17.7% had 2 induced abortions, 14.6% had 3 abortions, 10.3% had 4 abortions, and 0.2% (2cases) had over 20 abortions. Average was 2.7. 5) In regarding to the number of living children, the greatest number (45.0%) of clients had 3 children, and 26.5% 2 children, 19.7% 4 children. Average number of their living children was 3.03. 6) Sex ratio of living children, among 18 clients those had 1 child, 17 homemakers had 1 boy and no girl, 1 homemaker had no boy and 1 girl only. Sex ratio showed that woman who had 2 boys and no girl accounted for 46.3%, however, those having no boy and 2 girls ocmprised only 1. 8% among 229 clients who had 2 children. Among 389 clients who had 3 children, in spite of woman who had 3 boys and no girl comprised 16.5%, but no boy and 3 girls only 1.5%. Among 170 clients who had 4 children, homemakers with 4 boys and no girl accounted for 4.1%, however, no boy and 4 girls 1.8% of the total. Among 52 clients, who had 5 children, woman with 5 boys and no girl comprised 3.9%, no boy and 5 girls 0%. Among 7 cases who had 6 children, there were 3 cases who had 3 boys and 3 girls, but only 1 cases had 1 boy and 5 girls and so on. These results showed a strong trend of male preference in Korea and this could be one of the inhibit factors for family planning.
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金知和 이화여자대학교 가정대학 가정관리학과 1976 家政大學 敎授 論文集 Vol.1976 No.1
Careful scruting of the data indicate that malnutrition actually limits fertility. Spermatogenesis may likewise be impaired by inadequate diet, particularly one that is very poor in protein. For those who are underweight, increased caloric intake stressing high protein content is recommended. Included are supplementary vitamins, particularly B complex, which stimulate the appetite. Injudicious dieting by the woman to conform to current standards of beauty may also result in malnutrition. This contributes to faulty oogenesis and, in extreme dieting, may produce a long-standing amenorrhea. Obesity may also reduce fertility. Since most cases of obesity are due to over-eating, the full cooperation of the patient must be enlisted. And no device is effective for breaking up fatty deposits. Instead, a program of exercise is recommended. The treatment of both malnutrition and obesity is directed toward general dietary habits either weight gain or weight reduction, with a well balanced high protein diet.
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