고마리 내에서 Cu 의 흡수량과 Phytochelatin 의 관계

강경홍,김인성 ( Kyung Hong Kang,In Sung Kim ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.2

When Persicaria thunbergii was treated with Cu(NO_3)_2 of 5mM and 10 mM for 48hr in laboratory, the biolmagnification of copper in the leaf and root of plants and the rate of increase of phytochelatin were examined. The concentration of Cu in the root was greater than in the leaf and the Cu concentration in Cu(NO_3)_2 treated plants was higher than in Cu(NO_3)_2 5mM treated plants as follows; in the case of Cu 5mM treated plants, the leaf was 5.04㎍/g and the root was 11.25㎍/g and in 10 mM treated plants, the leaf was 6.96㎍/g and the root was 13.91㎍/g. But, in the extract of the soluble protein of the leaf and root tissue of plants with extraction buffer (20mM Tris-HCl, pH 8.0 and 10mM β-mercaptoethanol), the contcentration of Cu in the leaf was higher than in the root of plants. The phytochelatin (Cu-binding protein) was isolated from the tissue of leaf and root and chromatographed on the gel filtration resin Sephadex G100. The rate of increase of Cu-binding protein increased with increasing heavy-metal addition in nutrient solution as follows: Compared with that of the control, in the case of leaf, Cu 5mM treated extract was 202% and 10 mM treated extract was 222% and in root, Cu 5mM treated extract was 120% and 10 mM treated extract was 182%. And, it had a high UV absorption at 254 ㎚ and a low absorption at 280㎚.

하천유역의 관속식물에 의한 중금속의 생물학적농축 및 중금속 오염도 평가에 관한 연구 3. 고마리 ( Persicaria thunbergii ) 에 의한 서식처 토양의 Pb , Cu 및 Zn 의 제거

박기남,강경홍,김인성 ( Ki Nam Park,Kyung Hong Kang,In Sung Kim ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.2

The biological magnification and the uptake patterns of lead, copper and zinc in Persicaria thunbergii naturally grown at the areas of the rivers were investigated. The biological magnification values of lead, copper and zinc in the plant were 82.4∼254.6㎍g^1, 284.6∼688.4㎍g^1 and 635.5∼1979.4㎍g^1 individually. The tendency of the biological magnification in the each organ of the plant was as follows : Pb an Zn were increased of stem<leaf<root but Cu was leaf<stem<root. The amount of heavy metals in the plant were in the order of Pb<Cu<Zn. The magnification force of heavy metals in the plant were 13.2 times in Pb, 73.2 times in Cu and 58.7 times in Zn to compare with the content of heavy metals in the habitat soil and have a tendency to follow the content of those in the habitat soil but not proportional. In the mixture medium applied heavy metals, Cu induced the Zn depiciency in the plant. Concludly, the P. thunbergii was the excellent plant which removed the heavy metals from the soil and water of the habitat.

고마리에의한 농약성분의 생물학적 농축에 관한 연구

강경홍,김인성 Natural Science Research Institute JEONJU UNIVERSI 1995 전주대학교 자연과학연구소 학술논문집 Vol.8 No.2

In the study, the variations of biomass and biological magnification of the parathione and diazinon in Persicaria thunbergii cultured with the nutrient solutions added by the pesticides for 35 days were investigated. The results are as follows : The amount of the biological magnification of parathione and diazinon in the plant were reached to the highest values at 14 days, and then gradually declined. The biological magnifications of the plants treated with 5ppm, 10ppm and 20ppm of parathione were higher than those of diazinon by 1.27-5.92, 1.87-6.23 and 1.77-9.52 times, respectively. The biomass of the plants were declined as the growth times in which the pesticides were concentrated : those of the plants treated with 5, 10 and 20ppm of parathione were 0.81, 0.75 and 0.65 of the control, while those of diazinon were 0.96, 0.95 and 0.80 of the control, respectively.

The biological magnification of heavy metals and the assay of heavy metal pollution levels in the river area by vascular plants. : IV. The biological magnification of Pb, Cu, and Zn in Nymphides peltata and Trapa japonica IV. 노랑어리연꽃(Nymphides peltata)과 마름(Trapa japonica)에서 Pb, Cu 및 Zn의 생물학적농축

Kang, Kyung-Hong,Kim, In-Sung 전주대학교 자연과학종합연구소 1996 전주대학교 자연과학연구소 학술논문집 Vol.9 No.1

수생관속식물인 노랑어리연꽃(N. peltata)과 마름 (T japonica)에서 중금속의 생물학적 농축량과 서식지 물과 토양의 중금속 함류량과의 상관관계를 조사하였다. 노랑어리연꽃과 마름에서 Pb, Cu 및 Zn의 생물학적 농축량은 노랑어리연꽃보다 마름에서 더 큰 농축량을 나타냈고 두 관속식물에서의 중금속 농축량은 Pb<Cu<Zn의 순서로 증가하였다. 부위별 농축량은 두 수생관속식물 중 노랑어리연꽃에서는 Pb의 경우 잎<줄기<뿌리의 순서로 증가하였고 Cu와 Zn의 경우 줄기<잎<뿌리의 순서로 증가하였으며, 마름에서는 Pb, Cu 및 Zn 모두 식물제 내에서 잎<줄기<뿌리의 순서로 농축량이 증가하였다. 통계처리 결과 노랑어린연꽃의 뿌리에 농축된 Cu와 Zn의 농축량은 서식지 토양의 함량과 정상관관계를 나타냈고, 마름의 경우에서는 Pb와 Cu가 정상관관계를 나타냈다. 노랑어리연꽃과 마름에서 중금속 농축력은 서식지 토양의 중금속 함량에 비해 노랑어리연꽃의 경우 Pb는 15.5배, Cu는 37.9배, Zn는 87.4배로 나타났고, 마름의 경우 Pb는 26.3배, Cu는 109.2배 그리고 Zn는 183.6배로 나타났다.

고마리와 소리쟁이에 의한 Cd^2+ 와 Pb^2+의 흡수 및 phytochelatin에 관한 연구

강경홍,김인성,구정숙 한국환경생물학회 2000 환경생물 : 환경생물학회지 Vol.18 No.2

고마리와 소리쟁이에 Cd^2+와 Pb^2+를 각각 5 및 10mM로 5일간 처리한 결과, 고마리에서는 중금속 5mM 처리구의 경우 Pb^2+가 Cd^2+보다 약 3.5배, 10mM의 경우 약 2.9배로 Pb^2+의 농축량이 높게 검출된 반면, 소리쟁이는 Cd^2+ 5mM에서 약 1.49㎍/g, 10mM에서 약 2.90㎍/g 그리고 Pb^2+ 5mM에서 약 1.83㎍/g, 10mM에서 약 2.73㎍/g로 검출되어 처리농도별 Cd^2+와 Pb^2+의 농축량은 비슷하였다. 고마리와 소리쟁의 배양액(pH 6.5)에 Cd^2+와 Pb^2+를 5 및 10mM로 혼합하여 처리한 후 각 실험구 토양의 중금속 잔류율과 pH는 대조구에 비해 고마리를 배양하며 Cd^2+ 5mM을 처리한 경우 약 77.1%와 pH 6.39, 10mM을 처리한 경우 약 90.2%와 pH 5.97 그리고 Pb^2+ 5mM을 처리한 경우 약 81.1%와 pH 6.00, 10mM을 처리한 경우 약 85.7%와 pH 5.80, 소리쟁이를 배양하며 Cd^2+ 5mM을 처리한 경우 약 83.9%와 pH 6.32, 10mM을 처리한 경우 약 93.7%와 pH6.12 그리고 Pb^2+ 5mM을 처리한 경우 약 88.6%와 pH6.27,10mM을 처리한 경우 약 90.0%와 pH 6.02 정도였다. Phytochelatin은 고마리와 소리쟁이에서 모두 Cd^2+와 Pb^2+ 무처리구에 비해 5와 10mM 처리구에서 유도되었음을 확인하였다. 또한, 각 식물재료 내에서 중금속에 의해 유도된 phytochelatin의 분자량은 고마리의 경우 Cd^2+에 의해서는 약 4,300∼8,600 da, Pb^2+에 의해서는 약 3,200∼9,700 da, 소리쟁이의 경우 Cd^2+에서는 약 4,300 da, Pb^2+에 의해서는 약 3,200∼7,500 da 정도였다. When Persicaria thunbergii and Rumex crispus were treated with Cd(NO_3)_2 and Pb(NO_3)_2 of 5 or 10 mM for 5 days, the amount of bioaccumulation of Pb^2+ in the leaf of P. thunbergii was 2.87∼8.08㎍/g and that of Cd^2+ was 0.82∼2.97㎍/g. In the case of P. thunvergii, the concentration of Pb^2+ in the leaf was higher than that of Cd^2+. On the other hand, in R. crispus, the concentration of Cd^2+ and Pb^2+ were similar as follows ; 1.49㎍/g in Cd^2+ 5mM, 2.90㎍/g in Cd^2+ 10mM, 1.83㎍/g in Pb^2+ 5mM and 2.73㎍/g in Pb^2+ 10mM. The remaining rate of heavy metals and the variation of pH in the cultured soil decreased as compared with control(100% and pH 6.48) after 5 days as follows; to 77.1% and pH 6.39 in Cd^2+ 5mM, 90.2% and pH 5.79 in Cd^2+ 10mM, 81.1% and pH 6.00 in Pb^2+ 5mM, and 85.7% and pH 5.80 in Pb^2+ 10mM. The result of size exclusion chromatography, several phytochelatins were seperated from the extract of the leaf of both plants treated with heavy metals. The molecular mass of these phytochelatins were estimated as follows; in the case of P. thunbergii, about 4,300∼8,600 da by Cd^2+ and about 3,200∼9,700 da by Pb^2+, and in R. crispus, about 4,300 da by Cd^2+ and about 3,200∼7,500 da by Pb^2+. In addition, A_254 of these phytochelatins were higher than A_280. [Phytochelatin, Persicaria thunbergii, Rumex crispus]

하천유역의 관속식물에 의한 중금속의 생물학적농축 및 중금속 오염도 평가에 관한 연구 : I. 수생관속식물인 마름(Trapa japonica)의 중금속 Pb, Cu, Zn 및 Fe의 생물학적 농축 및 토양과 수질 내의 중금속 함량과의 관계 The biological magnification of Pb, Cu, Zn and Fe in water chestnut, Trapa japonica and relationship to metal contents in the adjacent river soil or water column

박기남,강경홍,김인성 Natural Science Research Institute JEONJU UNIVERSI 1995 전주대학교 자연과학연구소 학술논문집 Vol.8 No.1

The biological magnification of Pb, Cu, Zn and Fe from soil or water column in water chestnut, Trapa japonica and relationship between the concentration of Pb, Cu, Zn and Fe in Trapa japonica and in adjacent soil or water column were investigated in the Chonju river, Chonju city and suburb's river. At each of 5 sampling sites, 5 Trapa japonica individuals, water and soil were collected in every week during 4 months, separated into leaf, stem and root portions, stored in a deep freezer at -70℃ and analysed for total Pb, Cu, Zn and Fe by Trace Element Analyser 3000. The amounts of biological magnification of heavy metal in Trapa japonica were Pb(213.9㎍/g), Cu(618.1㎍/g), Zn(2346.7㎍/g) and Fe(6078.5㎍/g). The concentration of Pb, Cu, Zn and Fe in the leaf, stem and root of Trapa japonica was founded that concentration in leaf and stem was increased in the sequence Pb<Cu<Fe<Zn and in root was increased in the sequence Pb<Cu<Zn<Fe. The amount of biological magnification in the leaf, stem and root of Trapa japonica was generally increased in the sequence leaf<stem<root. Statistical Analysis suggested that the significant portion of Pb, Cu or Zn Content in Trapa japonica was derived from soil. The concentration of Pb, Cu and Zn in Trapa japonica relatively indicates the heavy metal content of soil in river area.