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벼 Oryza sativa × O. minuta 여교배 계통에서 이입 염색체단편 검정
김봉학,안상낙,강경호,권수진,정오영,리흥린,문헌팔 한국작물학회 2004 한국작물학회지 Vol.49 No.6
An introgression line, WH79006 was produced from a single plant from BC5F3 families from a cross between Hwaseongbyeo used as a recurrent parent and O. minuta (BBCC, Acc. No. 101154) as a donor parent, which was subsequently self-pollinated for three generations. WH79006 resembled the O. sativa parent, Hwaseongbyeo. However it differed from Hwaseongbyeo in several traits including days to heading, culm length, grain size, spikelets per panicle and fertility. These differences in the traits between WH79006 and Hwaseongbyeo can be attributed to the O. minuta introgressions. To detect the introgressions, 294 SSR markers of known chromosomal position have been used. At least, 28 introgressed chromosomal segments have been identified using SSR markers and they map to all chromosomes except chromosome 2. The size of the introgressed segments ranged from 4 to 35cM. A QTL related to culm length was detected using 75 F2 plants from the Hwaseongbyeo / WH79006 cross. This QTL, cl6 located on chromosome 6 explained 9.6% of the total phenotypic variation in the population. This QTL has not been detected in the previous QTL studies between Oryza cultivars, indicating potentially novel alleles from O. minuta. 화성벼와 O. minuta 간 여교잡을 통해 반복친인 화성벼와 출수기, 간장 등 여러 작물학적 특성에서 뚜렷한 차이를 보이는 염색체단편 치환계통 “WH79006”을 육성하였는데 화성벼와 WH79006의 차이는 WH79006에 이입된 O. minuta 염색체 단편에 의한 것이라고 할 수 있다. WH79006이 화성벼의 유전적 배경에 O. minuta의 어느 염색체 단편을 가지고 있는지를 검정하기 위해 SSR 마커를 이용하였다. 벼 염색체에 골고루 분포한 294개의 SSR 마커를 검정한 결과 최소한 28개의 이입 단편을 검정하였는데 이들은 2번 염색체를 제외한 모든 염색체에 분포하였으며 전체 길이는 약 168cM이었다. 간장 관여 QTL을 탐색하기 위해 화성벼/WH79006 조합의 75개 F2 개체를 육성, 간장을 조사하였다. QTL 분석 결과 6번 염색체의 RM225 부근에서 간장에 관여하는 QTL cl6가 탐지되었다. cl6는 전체 표현형변이의 9.6%를 설명하였으며 O. minuta의 대립유전자가 간장을 크게 하는 방향으로 작용하였다. 이 결과는 O. minuta가 포복형임에도 불구하고 간장 조절 유전자들 중에는 간장을 크게 하는 방향으로 작용하는 유전자가 있음을 제시하고 있으며, 이 유전자들은 앞으로 QTL 분석을 통해 그 작용을 밝힐 예정이다. 추후 교잡 집단을 이용 O. minuta 특이적 단편들이 어느 형질과 관련이 있는지를 밝힐 예정이다.
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유용심,김봉학,Yoo, Yong Shim,Kim, Bong-Hak 한국광학회 2018 한국광학회지 Vol.29 No.2
Comparison calibration equipment for infrared-radiation thermometers below $0^{\circ}C$ has been established, using a TRT2 (transfer radiation thermometer 2, HEITRONICS) as a transfer standard and an ME30 (Model: ME30, HEITRONICS) as a variabletemperature blackbody. The TRT2 was calibrated using three fixed points (Ice ($0.01^{\circ}C$), In ($156.5985^{\circ}C$), and Sn ($231.928^{\circ}C$)) and the Planckian Sakuma-Hattori equation, and including the interpolation and extrapolation errors at $-50^{\circ}C$ in the uncertainty. The pneumatic lid is installed upon opening of the ME30 and is opened for only 30 seconds for measuring the radiation temperature, which prevents formation of ice in the ME30 and also reduces the calibration time to half. The farther away from the $0{\sim}232^{\circ}C$ region, the larger the uncertainty of the comparison calibration equipment becomes. The expanded uncertainty of the comparison calibration equipment was estimated as 0.26 K at $-20^{\circ}C$.
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유용심,김봉학,박철웅,박승남,Yoo, Yong-Shim,Kim, Bong-Hwak,Park, Chul-Woung,Park, Seung-Nam 한국광학회 2010 한국광학회지 Vol.21 No.4
All radiation thermometers have a size-of-source effect (SSE) and a self-radiation effect (SRE). The SSE,defined as dependence of the detector signal of a radiation thermometer on the diameter of a source, is critically dependent on the wavelength since diffraction is the main cause. In this paper, we have measured the SSE and the SRE of TRT2 (Transfer Radiation Thermometer 2, HEITRONICS) widely used as a transfer standard in low and middle temperature range. At $300^{\circ}C$, The radiation temperature difference between the 60 mm diameter blackbody and 10 mm diameter blackbody due to the SSE was estimated to be $3.5^{\circ}C$ in low temperature mode ($8-14\;{\mu}m$) and $0.5^{\circ}C$ in middle temperature mode ($3.9\;{\mu}m$). In addition, the measured radiation temperature difference of the blackbody due to the SRE was found to be 110 mK when the body temperature change of TRT2 was set at $2.6^{\circ}C$.
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박철웅,김봉학,박승남 한국물리학회 2005 새물리 Vol.50 No.1
We used a variable temperature blackbody furnace to construct a nickel-carbon eutectic fixed-point cell for a temperature standard, and we determined the melting and the freezing temperatures by using a radiation thermometer with two spectral bands, 650 nm and 850 nm. The furnace has a cylindrical graphite tube heated directly by the current. The temperature uniformity along the graphite tube was less than $\pm$ 7 $^\circ$C in the central region where the cell was located. The melting temperature obtained at the minimum derivatives of the plateaus was 1328.71 $^\circ$C with an expanded uncertainty of 0.22 $^\circ$C ($k$ = 2). The uncertainty of the temperature determination was estimated by considering the uncertainty components from the reference temperature of a copper fixed point blackbody, the eutectic point realization, and the radiation thermometer. 니켈-탄소 공융점을 가변 고온 흑체로를 이용하여 제작하고 녹는점과 어는점의 온도를 복사온도계의 650 nm 와 850 nm 의 두 가지 파장영역에서 측정하였다. 가변 고온 흑연관 흑체로는 원통형 흑연관을 직접 가열하는 형태로 제작되었고, 장치의 온도분포의 균일도는 중심에서 $\pm$ 7 $^\circ$C 이내였으며, 안정도는 0.03 $^\circ$C 이내였다. 기울기가 최소인 지점에서 측정한 공융점의 녹는점 온도는 1328.71 $^\circ$C 이었고 측정 결과의 확장 불확도($k$ = 2)는 0.22 $^\circ$C였다. 녹는점 온도결정 시 확장 불확도는 구리점 교정 불확도, 공융점 실현 불확도, 복사 온도계 측정불확도를 포함하여 결정하였다.
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서정철,박승남,김봉학 한국광학회 2001 한국광학회지 Vol.12 No.5
We have fabricated a spectroadiometric system to measure spectral radiance of optical sources and evaluated its characteristics such as spectral responsivity, nonlinearity, and so on. The measurement system with PMT, Si, InGaAs, and IR-enhanced InGaAs detectors has shown a good linearity and a wide spectral responsivity of 250∼2500 nm. This spectroradiometric system will be used as the primary national standard system of spectral radiance measurements.
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