괄루인 렉틴의 특성

김경순,최근호,박종옥 ( Kyungsoon Kim,Gunho Choi Jong Ok Park ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.5

Trichosanthis fructus Lectin (TFL) was partially purified by ammonium sulfate fractionation, heat treatment, DEAF-Sephadex ion exchange chromatography and Sephadex G-200 gel chromatography. The molecular weight of TFL was estimated by gel filtration and SDS-PAGE. The Molecular size of the lectin was determined to be 67,000 dalton, which was composed of two subunits having molecular weights of 34,000 and 35,000 dalton, respectively. The effect of sugars and metal ions on the hemagglutinating activity of lectin was also examined. The hemagglutinating activity of the lectin was inhibited by some sugars (β-Lactose, α-Lactose, D-(+)-Galactose) and metal ions (Hg^(2+), Cu^(2+), Mg^(2+), Ca^(2+), pb^(2+)).

모유수유와 분유수유에 따른 영아 장내 미생물 군집의 특징

김경순,신정,심지수,연수지,이평안,정문규,Kim, Kyeong Soon,Shin, Jung,Sim, JiSoo,Yeon, SuJi,Lee, Pyeong An,Chung, Moon Gyu 한국미생물학회 2019 미생물학회지 Vol.55 No.3

장 내에 존재하는 microbiome은 생활환경, 나이와 섭취하는 음식물에 따라 변하게 된다. 본논문에서는 모유를 섭취한 영아와 분유를 섭취한 영아들에 대한 장내미생물 군집의 변화를 비교하였다. 미생물의 군집변화를 차세대 염기서열 분석기법을 이용하여 분석하였으며 총 80개의 영아 분변을 통해 미생물 군집변화를 확인하였다. 모유그룹(BIG)과 분유그룹(FIG-A, FIG-B, FIG-C)간의 장내세균의 군집을 비교한 결과 BIG에서는 Actinobacteria 문이 전체 군집의 $74.22{\pm}3.48%$로 우점을 차지하였다. 분유그룹의 Actinobacteria 문을 비교하였을 때 FIG-A는 $73.46{\pm}4.12%$였지만 FIG-B와 FIG-C는 $66.52{\pm}5.80%$와 $68.88{\pm}4.33%$로 BIG와 FIG-A에 비해 낮은 비율을 보였다. 속(genus) 수준에서 살펴보면 Bifidobacterium이 전체 미생물군집에서 가장 높은 비율로 분포하고 있었으며 BIG가 $73.09{\pm}2.31%$로 가장 높았고 FIG-A, FIG-B 및 FIG-C는 각각 $72.25{\pm}4.93%$, $63.81{\pm}6.05%$ 및 $67.42{\pm}5.36%$를 차지하였다. Bifidobacterium 종의 경우 모든 그룹에서 Bifidobacterium longum이 우점을 차지하고 있었으며 BIG와 FIG-A가 전체 군집의 $68.77{\pm}6.07%$와 $66.85{\pm}5.80%$를 차지하였다. 이에 반해 FIG-B와 FIG-C는 각각 $58.94{\pm}6.20%$와 $61.86{\pm}5.31%$로 BIG와 FIG-A보다 낮은 비율로 분포하는 것을 분석하였다. FIG-A가 섭취한 분유는 Bifidobacterium의 선택적 증식이 우수하다고 알려진 갈락토올리고당, 갈락토실락토스, 시너지올리고당, 비피도올리고 및 장내균총개선소재를 혼합한 5-Bifidus factor 복합물이 포함되어 있다. 이와 같은 5-Bifidus factor라는 유산균에 대한 증식능이 우수한 프리바이오틱스를 혼합하였기 때문에 모유를 섭취한 영아의 장내에 존재하는 Bifidobacterium 속의 군집과 유사하게 나타난 것으로 판단된다. The intestinal microbiomes vary according to the factors such environment, age and diet. The purpose of this study was to compare the gut microbial diversity between Korean infants receiving breast-fed milk and formula-fed milk. We analyzed microbial communities in stool samples collected from 80 Korean infants using next generation sequencing. Phylum level analysis revealed that microbial communities in both breast-fed infants group (BIG) was dominated by Actinobacteria ($74.22{\pm}3.48%$). Interestingly, the phylum Actinobacteria was dominant in formula-fed infants group A (FIG-A) at $73.46{\pm}4.12%$, but the proportions of phylum Actinobacteria were lower in formulafed infants group B and C (FIG-B and FIG-C) at $66.52{\pm}5.80%$ and $68.88{\pm}4.33%$. The most abundant genus in the BIG, FIG-A, FIG-B, and FIG-C was Bifidobacterium, comprising $73.09{\pm}2.31%$, $72.25{\pm}4.93%$, $63.81{\pm}6.05%$, and $67.42{\pm}5.36%$ of the total bacteria. Furthermore, the dominant bifidobacterial species detected in BIG and FIG-A was Bifidobacterium longum at $68.77{\pm}6.07%$ and $66.85{\pm}4.99%$ of the total bacteria. In contrast, the proportions of B. longum of FIG-B and FIG-C were $58.94{\pm}6.20%$ and $61.86{\pm}5.31%$ of the total bacteria. FIG-A showed a community similar to BIG, which may be due to the inclusion of galactooligosaccharide, galactosyllactose, synergy-oligosaccharide, bifidooligo and improvement material of gut microbiota contained in formula-milk. We conclude that 5-Bifidus factor contained in milk powder promotes the growth of Bifidobacterium genus in the intestines.

항암플러스 투여로 호전된 진행성 비소세포성 폐암 연속환자증례

김경순,정태영,유화승,이연월,조종관,Kim, Kyung-Soon,Jung, Tae-Young,Yoo, Hwa-Seung,Lee, Yeon-Weol,Cho, Chong-Kwan 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.4

Objective : To investigate the therapeutic effects of Hang-Am-Plus (HAP) on advanced non-small cell lung cancer (NSCLC) patients. Method : We prescribed HAP three times a day (3,000-6,000 mg/ day) during the treatment period (8 - 24 months). Computed tomography (CT) was performed to evaluate the therapeutic efficacy. Results : Four patients who were diagnosed with NSCLC were recommended chemotherap y, but refused it because of old age, side effects, or treatment failure, and instead sought oriental medicinal therapy. They were treated around 12 months. The patients showed stable disease (SD) state for 5 months, 3 months, 19 months and 3 months, respectively. Conclusion : These four cases may give us the possibility that HAP offers potential benefits for non-small cell lung cancer patients.

비유를 사용한 수업에서 학생들의 인지적.정의적 특성과 대응 이해 및 대응 오류 유형과의 관계

김경순,황선영,노태희,Kim, Kyung-Sun,Hwang, Sun-Young,Noh, Tae-Hee 대한화학회 2010 대한화학회지 Vol.54 No.1

이 연구에서는 비유를 사용한 '농도와 반응속도'에 대한 학습에서 학생들의 인지적 정의적 변인들의 상 하위 수준에 따른 대응 관계 이해도와 대응 오류 유형의 차이를 비교하고, 이 변인들과 대응 관계 이해도와의 관계를 조사하였다. 사전검사로 논리적 사고력, 시각적 심상화 능력, 비유 추론 능력, 자아 효능감, 인지욕구 검사를 실시하였다. 비유를 사용한 수업을 진행한 후, 친숙도 검사와 대응 관계 이해도 검사를 실시하였다. 연구 결과, 시각적 심상화 능력과 친숙도를 제외한 모든 인지적 정의적 변인의 수준이 상위인 학생들의 대응 관계 이해도 점수가 하위 학생들보다 통계적으로 유의미하게 높았다. 또한, 과잉 대응, 대응 불이행, 불가능한 대응, 인위적 대응, 부적절한 대응, 무분별한 대응, 비유물 속성 보유 등의 대응 오류 유형의 빈도가 학생들의 인지적 정의적 변인의 수준에 따라 다른 양상을 보였다. 학생들의 대응 관계 이해도 점수는 모든 인지적 정의적 변인들과 정적인 상관이 있었다. 다중 회귀 분석 결과, 과학 성취도, 논리적 사고력, 친숙도가 대응 관계 이해도에 대한 유의미한 예언 변인으로 나타났다. 이에 대한 교육적 함의를 논의하였다. In this study, we investigated the differences of mapping understanding and the types of mapping errors by the levels of students' cognitive/affective variables and the relationships between mapping understanding and these variables in learning 'concentration and reaction rate' with analogy. After administering the tests regarding logical thinking ability, visual imagery ability, analogical reasoning ability, self efficacy, and need for cognition as pretests, students learned with analogy. Then, students' familiarity and mapping understanding were examined. Analyses of the results revealed that the scores of the mapping understanding for the students with higher levels of all cognitive/affective variables except visual imagery ability and familiarity were significantly higher than those for the students with lower levels. The differences in the types of the mapping errors such as overmapping, failure to map, impossible mapping, artificial mapping, mismapping, rash mapping, and retention of a base feature were also found by the levels of students' cognitive and affective variables. The scores of students' mapping understanding were positively correlated with those of all cognitive and affective variables. The results of multiple regression analysis indicated that students' science achievement, logical thinking ability, and familiarity were significant predictors of mapping understanding. Educational implications of these findings are discussed.

Squamous Cell carcinoma cell line 에서 cellular proto - oncogene 들의 증폭 및 발현

김경순,박노희 ( Kyung Soon Kim,No Hee Park ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.2

The restriction profiles and expression of various cellular proto-oncogenes in normal human epidermal keratinocytes and human syuamous cell carcinoma cell lines were studied. Both normal human epidermal keratinocytes and cancer cell lines contained and expressed various proto-oncogenes such as erb-B-I, abl, fos, raf, H-ras, and myc. Although the Eco RI restriction profiles of the oncogenes from the normal cells and cancer cells were similar, the amount of the oncogene in the cancer cell lines was generally higher than that in the NHEK. Furthermore, the amount of expression of these proto-oncogenes was significantly higher in the cancer cell lines than in the normal keratinocytes. These data indicate that the overexpression of several cellular proto-oncogenes might be necessary for the malignant transformation and maintenance of the malignancies of syuamous cell carcinoma.

Studies on the Ornithine Aminotransferase from Chicken Liver

김경순,박종옥,박광숙,Kim, Gyung-Soon,Park, Jong-Ok,Park, Kwang-Sook 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2

닭 간에서 ornitine aminotransferase는 주로 mitochondrial fraction에 존재하였다. 이 효소를 열처리, ammonium sulfate fractionation, DEAE-cellulose ion exchange chromatography, Sephadex G-200 filtration, CM-Sephadex column chromatography를 이용하여 정제해서 disc polyacrylamide gel electrophoresis를 하였더니 하나의 단백질 띠가 얻어졌다. 이 정제된 효소를 이용해서 몇가지 성질을 조사해본 결과는 다음과 갈다. SDS-polyacrylamide gel electrophoresis에 의해 측정된 분자량은 62,000이었고 gel filtration에 의한 값은 63,000이었으므로 이 효소는 monomer로 존재한다고 생각되었다. L-ornithine과 $\alpha$-ketoglutarate에 대한 $K_m$ 값은 각각 6.31 mM, 1, 03 mM 이였으며, 이 효소는 기질의 농도가 높을 때 저해되었고 또한 3 mM p-chloromercuriphenylsulfonic acid를 가해주면 80% 저해되었다. glyoxylate, oxaloacetate, pyruvate도 L-ornithine보다는 못하지만 amino group acceptor로서 작용하였으며, 효소활성에 대한 최적 pH는 7.3으로 측정되었다. Ornithine aminotransferase was purified from chicken liver and several properties of the enzyme were studied. The purified enzyme appeared to be homogeneous on polyacrylamide gel electrophoresis with and without SDS. The molecular weight of the enzyme was estimated as 62,000 by SDS-polyacrylamide gel electrophoresis, and as 63,000 by gel filtration. The $K_m$ values for ornithine and $\alpha$-ketoglutarate were found to be 6.31 mM and 1.03 mM, respectively. The enzyme was inhibited either by high substrate concentrations or by p-chloromercuriphenylsulfonic acid. Glyoxylate, oxaloacetate and pyruvate could act as amino group acceptors, but they were much less effective than $\alpha$-ketoglutarate. Optimum pH of this enzyme was found to be 7.3.

항암단 투여로 호전된 진행성 또는 전이성 담낭암 연속환자증례

김경순,유화승,정태영,이연월,조종관,Kim, Kyung-Soon,Yoo, Hwa-Seung,Jung, Tae-Young,Lee, Yeon-Weol,Cho, Chong-Kwan 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.3

Objective : To investigate the therapeutic effects of Hang-Am-Dan(HAD) on gallbladder cancer patients. Method : We prescribed HAD three times a day(3,000-6,000 mg/day) during a treatment period of 8 and 34 months respectively. Computed tomography(CT) was taken to evaluate the therapeutic efficacy. Results : Two patients diagnosed with gallbladder cancer were recommended to go through chemotherapy. However, they refused the standard regimen due to their old age and the fear of side effects, and chose to go with Oriental medicine treatments instead. The patients were treated for 8 and 34 months respectively. Each patient showed stable disease(SD) state during the treatment period. Conclusion : According to these cases, it could be concluded that HAD may offer potential benefits in treating gallbladder cancer patients.

귀전우, 백화사설초, 와송 추출물을 처치한 난소암과 자궁경부암 세포에서의 단백질 발현 변화

김경순,예성철,유병철,조종관,이연월,유화승,Kim, Kyung-Soon,Yea, Sung-Chul,Yoo, Byong-Chul,Cho, Chong-Kwan,Lee, Yeon-Weol,Yoo, Hwa-Seung 대한한방내과학회 2011 大韓韓方內科學會誌 Vol.32 No.1

Background : Despite recent advances in cancer management, prognosis of ovarian cancer is poor. Anticancer effects of herbal medicine, such as Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger, have been reported in treatment of ovarian and cervical cancers, but the systematic approaches to explain their molecular mechanism(s) have not yet been established. Objectives : To establish a basis of understanding for anti-cancer mechanisms of herbal medicine, we profiled protein expression in human ovarian and cervical cancer cells treated with the extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh and Orostachys japonicus A. Berger. Methods : Human ovarian cancer cell line NIH:OVCAR-3, and human cervical cancer cell line HeLa were employed in the present study. Whole protein was obtained from the cells harvested at 48 hours after the treatment with herbal water-extract, and analyzed by 2DE-based proteomic approach. Results : Various changes of protein expression induced by the herbal treatment were monitored : down-regulation of molecular chaperone (calreticulin variant), glycolytic enzymes (D-3-phosphoglycerate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase and alpha-enolase), RNA processing molecules (hnRNP A2/B1), and antioxidant protein (peroxiredoxin 1). Conclusions : Repression of glycolysis has been accepted as the mechanism to increase anticancer reagent's effect. Thus, down-regulation of glycolytic enzymes by the herbal extracts suggested a possible synergistic effect of herbs in the presence of platinum-based therapeutics. In further study, as well as the synergistic effect of the herbs, it has to be further validated whether artificial regulation of hnRNP A2/B1 in ovarian cancer cells affects various cancer survival factors, since RNA processing can be interrupted by deranged expression of hnRNP subtypes, and it results in an inhibition of cancer cell growth.

2009 개정 교육과정에 따른 중학교 과학 교과서의 화학 영역과 고등학교 화학 교과서에 사용된 비유의 분석

김경순,안인영,최용남,노태희,Kim, Kyungsun,Ahn, Inyoung,Choi, Yongnam,Noh, Taehee 대한화학회 2013 대한화학회지 Vol.57 No.6

In this study, we analyzed the analogies in the chemistry domain of 27 middle school science textbooks and 8 high school chemistry textbooks developed under the 2009 Revised National Curriculum. A total of 284 analogies (science, 143; chemistry, 141) were identified from the textbooks, which means that 8.11 analogies (science, 5.3; chemistry, 17.6) per textbook and 0.68 analogy (science, 0.66; chemistry, 0.70) per 10 pages were used on average. Compared with previous curricula that the number of analogies gradually increased, the use of analogy was found to be somewhat decreased in the 2009 Revised National Curriculum. The number of analogies found in each textbook considerably varied depending on course, unit, and publishing company. One hundred ninety one different kinds of analogies were used, among which 38(19.9%) were used over twice. Further analyses of the types of analogies indicated that verbal and pictorial analogy, analogy with abstract target and concrete analog, and enriched analogy were frequently used. However, the term 'analogy' and the description about the limitation of the analogy were rarely mentioned. Artificial analogy, teacher-centered analogy, and analogy with low systematicity were also found to be frequently used. Educational implications of these findings are discussed. 이 연구에서는 2009 개정 교육과정에 따른 중학교 과학 교과서 27권의 화학 영역과 고등학교 화학 교과서 8권에서 사용된 비유의 빈도와 유형을 분석하였다. 분석한 교과서에 사용된 비유는 총 284개(과학, 143개; 화학, 141개)로서, 교과서 한 권당 8.11개(과학, 5.3개; 화학, 17.6개), 10쪽마다 평균 0.68개(과학, 0.66개; 화학, 0.70개)의 비유가 사용되었다. 비유의 빈도는 이전 교육과정까지는 꾸준히 증가하는 추세였던 것과는 달리 2009 개정 교육과정에서 다소 감소하였다. 또한, 교과목별, 단원별, 출판사별로 사용된 비유의 빈도에 차이가 나타났다. 비유물의 종류는 총 191종(과학, 81종; 화학, 110종)이었고, 각 교과목별로 2번 이상 공통으로 사용된 비유물은 38종(19.9%)이였다. 유형별로 비유를 분석한 결과, 그림과 언어를 같이 이용한 비유 및 추상적 개념을 구체적 비유물에 대응시키는 비유가 주로 사용되었고, 공유 속성을 설명해 주는 부연 비유의 사용이 많았다. 그러나 비유임을 명시하거나 비유물의 제한점에 대해 언급한 비유들이 거의 없었고, 체계성이 낮은 비유나 작위적 상황을 적용한 비유 및 교사 중심의 비유가 많이 사용되는 것으로 나타났다. 이 연구의 결과에 대한 교육적 함의를 논의하였다.

Amplification and Expression of Several Cellular Proto-Oncogenes in Squamous Cell Carcinoma Cell Lines

김경순,박노희,Kim, Kyung-Soon,Park, No-Hee 생화학분자생물학회 1990 한국생화학회지 Vol.23 No.2

사람의 정상적 epidermal keratinocyte(NHEK)와 사람의 squamous cell carcinoma cell line에서 여러 가지 cellular proto-oncogene의 존재, restriction profile, 그리고 expession을 조사하였다. NHEK와 cell line들로부터 얻은 발암유전자의 Eco RI restriction profile은 유사하였고 정상세포와 암세포들은 모두 몇 가지 proto-oncogene들을 발현하였으나 NHEK에 비해 암세포에서 이들 proto-oncogene의 발현되는 양이 많았다. 이 결과로부터 squamous cell carcinoma의 malignant transformation과 그 유지에 있어서 몇 가지 cellular proto-oncogene들의 과발현이 필요하다는 것을 알 수 있다. The restriction profiles and expression of various cellular proto-oncogenes in normal human epidermal keratinocytes and human squamous cell carcinoma cell lines were studied. Both normal human epidermal keratinocytes and cancer cell lines contained and expressed various proto-oncogenes such as erb-B-1, abl, fos, raf, H-ras, and myc. Although the Eco RI restriction profiles of the oncogenes from the normal cells and cancer cells were similar, the amount of the oncogene in the cancer cell lines was generally higher than that in the NHEK. Furthermore, the amount of expression of these proto-oncogenes was significantly higher in the cancer cell lines than in the normal keratinocytes. These data indicate that the overexpression of several cellular proto-oncogenes might be necessary for the malignant transformation and maintenance of the malignancies of squamous cell carcinoma.