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국윤민,강영미,문성환,고원건 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.38 No.-
The combination of electrospinning and the subsequent gelation of alginate produced bi-compartmentalhydrogel consisting of a nanofiber-incorporated hydrogel matrix domain and a bare alginate hydrogeldomain. The co-culture system was prepared by placing intervertebral disk (IVD) cells in the barealginate hydrogel and human bone marrow mesenchymal stem cells (hMSCs) in the nanofiberincorporatedhydrogel. Real-time polymerase chain reaction (PCR), western blotting, immunofluorescencestaining, and sulfated glycosaminoglycan assays revealed that the co-cultured groups producedmore collagen type II, aggrecan, glucose transporter-1 (GLUT-1), and glycosaminoglycans (GAG) than thesingle-cultured hMSCs, confirming the enhanced differentiation of hMSCs in the co-culture system. It isexpected that our bi-compartmental 3D scaffold can be applied to heterotypic co-culture systems for thestudy of various cell–cell interactions.