비교유전자 보합법의 최적조건들에 관한 연구

구선회,전영미 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

With the recent rapid expansion in the use of the comparative genomic hybridization(CGH) technique, increased atttention to improve the quality of technique is essential. In the present study, we performed the optimized condition of CGH technique. 1) The most important procedures were metaphase slide preparation and denaturation. The most appropriate method of slide making was the dropping of the cell suspension onto a slide at 15-30°angle lengthwise and relative humidity adjusted to 60-70% during slide prepartion 2) For preparation of cytoplasm-free metaphase spreads, freshly dropped slide was immersed for 1-2 seconds in the fresh fixative. 3) The most effective denaturation time and temperature was at 75℃ for 2min, after aging at 65℃ for overnight. 4) Duration of hybridization time did not affect the results of hybridization.

Extended-spectrum β-lactamase에 의한 항균제 내성

구선회 대한화학요법학회 1998 대한화학요법학회지 Vol.16 No.3

뇌종양세포의 세포유전학적 고찰

구선회,임춘화,전영미,김성호 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

Cytogenetic results and genetic alterations are closely related with tumorigenesis, progression and promotion of cancers. With the deepening of basic biologic understanding of neoplastic mechanisms, the clinical usefulness of various cytogenetic abnormalities as diagnostic and prognostic aids has been appreciated. Ten cases of brain tumors(2 Glioblastoma multiforme, 3 meningioma, 1 ependymoma, 1 gliosarcoma, 1 astrocytoma, and 1 metastatic tumor) were studied and 8 cases were possible for karyotyping . Four patients showed abnormal karyotypes. Structural and numerical abnormalities were detected. Karyotyping of 2 Glioblastoma multiforme showed trisomy 7, loss of chromosome 10 and loss of chromosome 8. Structural anomalies were detected on chromosome 1q, and 7. Many many marker chromosomes were also detected. One gliosarcoma showed many numerical anomalies, such as +7, +8, +17, and +mar. Meningioma cells studied were normal daryotyping. Although the cases were too small to correlate with the tumorigenesis, but histologically poor grade type of tumors showed very complex karyotypes.

Flow Cytometry를 이용한 Lymphocyte subset 측정에 관한 연구

구선회 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

Recently flow cytometry has been provide a wide range of application in routine clinical use, such as DNA content, platelet antibody, reticulocyte count and chromosomal study. Among this function, immunophenotyping of lymphocyte is the principal function of flow cytometry and its clinical relevance is already established for a number of disorders and is now being investigated for a variety of disorder. The fluorescent microscopic method had been used for lymphocyte subset but it has some drawback, it is time consuming, tedious, and analyze only limited number of cells and influenced by individual's variation. But flowcytometry rapidly analyze the individual cells in a very short time (1000 cells/sec) and can analyze in dual immunofluorescence staining in single cells. Also, only lymphocyte could be analyzed by gating technique. Forward and orthogonal light scatter and two fluorescence intensity were used to define the gate. A comparison was made between the subsets by fluorescence microscopy and flow cytometry in which correlation coefficients of more than 0.88. From the above results, we concluded that the flow cytometry was very useful for immunophenotyping of lymphocyte in large number of samples in a short time.

요로계 감염에서 Staphylococcus Saprophyticus의 검출에 관한 연구

구선회 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.2

S. saprophyticus has been considered contaminated bacteria. but recently it was reported that those organisms are capable of causing infection of urinary tract. So, the appreciation of the significance of coagulase negative staphylococci infection is being rapidly expanded. Thus, In clinical laboratories, it has become more important to accurately differentiate staphylococci from nonpathogenic micrococcus. This study was performed to investigate the status of infected patient with S. saprophyticus. Total 2,234 urine specimen was cultured and 71 young woman with urethritis symptom was studied by urine culture and urine sediment microscopy for evaluate the age prevalance. The results obtained are summarized as follows: 1. Among the total 2,234 urine culture, the growth the rate was 42.3%. The most common strain was E. coli and other Gram negative baccilli. The growth rate of S. saprophyticus was 0.2%. 2. Among the 71 young woman with urethritis symptom. The growth rate of S. saprophyticus was 4.2%. 3. The isolation of S. saprophyticus was from the patient with lung ca, sepsis, pregnancy and urethritis. Microscopic findings disclose epithelial cells and WBC. 4. Sensitivity pattern of S. saprophyticus disclosed that it was susceptible in penicillin, Sx, T., Ampicillin and sulfonamide, but resistant strain was founded in Amikacin, cephalothin, G. M. and K. M. From the above results, we could conclude that S. saprophyticus are not simple contaminant but important pathogen in young woman and other conditions. So, it need identify the S. saprophyticus by more simple and scientific method in clinical specimen.

MS-2 System과 Disk diffusion method를 이용한 항생제 감수성검사의 비교연구

구선회,박종우 대한임상병리학회 1984 대한임상병리학회지 Vol.4 No.2

적혈구 자가면역 항체에 대한 연구

구선회 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

The presence of autoantibody on RBC make diffculties in blood typing and crossmatching. In addition, If alloantibodies exist with autoantibody, transfusion reactions may be brought by masking the alloantibody. So it is important thing to identify the antibody specificity in blood bank working. We analysed 23 cases patients with auto and alloantibodies. The prevalence age with autoantibody were 10th and 50th. Antibodies identified in antiglobulin test were 4 cases of Rh specificities, and 1 case of kell specificity. Another 5 cases with warm antibodies were not identified. The Rh specificities consisted of 2 cases of anti-E, 1 case of anti-e and anti-c, 3 cases were cold antoantibody, bu the specificity were not identified. 6 case were idiopathic origin and other 7 case were secondary to other diseases. 3 cases show severe hemolysis, so transfusion was needed.

선천성 타원형 적혈구증 1예

구선회,박종우,권선호 大韓血液學會 1984 大韓血液學會誌 Vol.19 No.2

Antimicrobial Resistance and Multilocus Sequence Typing of Vancomycin-Resistant Enterococcus faecium Isolated from the Chungcheong Area

구선회,조혜현,성지연,권계철,임진숙 대한임상미생물학회 2011 Annals of clinical microbiology Vol.14 No.2

Background: Enterococcus faecium has emerged as an important nosocomial pathogen worldwide, and this trend has been associated with the dissemination of a genetic lineage designated clonal complex 17 (CC17). In the present study, characterization of the glycopeptide resistance mechanism, genetic relatedness, and pathogenicity in isolates of vancomycin-resistant E. faecium in the Chungcheong area were investigated. Methods: A total of 37 consecutive, non-duplicate, vancomycin-resistant E. faecium were isolated at three university hospitals in the Chungcheong area. The mechanism of glycopeptide resistance and pathogenicity factors were studied using PCR, and the genetic relatedness was determined via multilocus sequence type and esp repeat profile analysis. Additionally, the quinolone resistance-determining regions of parC and gyrA were sequenced to identify mutations involved in ciprofloxacin resistance. Results: Two genotypes of VRE were confirmed: VanAphenotype vanA genotype VRE (25 isolates) and VanB-phenotype vanA genotype VRE (12 isolates). MLST analysis revealed five sequence types. A significant result was that ST414 and CNS4 (4-1-1-1-1-1-1) were considered as belonging to CC17. The esp and hyl genes were found in 100% and 86.4% of the isolates, respectively. A total of 37 isolates showed genetic mutations in parC and gyrA. Conclusion: All isolated strains in the present study belonged to one of the CC17 genotypes including ST414 and CNS4 (4-1-1-1-1-1-1), which were not previously detected in Korea. The combination of MLST and the esp gene repeat profiles can be useful for genetic characterization of VREF isolates with regard to the evolutionary process and epidemiology of the clones.

암환자의 DNA 함량 측정에 관한 연구

구선회 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

Nuclear DNA content was measured by means of flow cytometry in 61 human solid tumor. The results are as follows. 1. There were aneuploidity in 48% of stomach cancer, 50% of rectosigmoid cancer, 40% of breast cancer, 66% of prostate cancer, 75% of bladder cancer, 20% of laryngeal cancer, 33 of neck mass of metastatic cancer, 100% of parotid gland tumor, 50% of tongue cancer and 50% of cervical cancer. 2. Brest tumors and laryngeal cancer manifest higher proliferative indices. From the above results, we could observe that the most of cancer cells have a high porliferative activity in quantitative analysis of DNA content. Therefore analysis of DNA content could be a one of the prognostic index in addition to histological classification of cancers.