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곽희진,계수경,곽희선,이경혜 동아시아식생활학회 2000 동아시아식생활학회지 Vol.10 No.4
To understand the effect of radish on growth of food born microorganisms, mashedflesh radishes were extracted by using acetone and distilled water. Their effect was assayed by measuring the optical density of cultural broth of food born microorganisms, In the experiment, seven strains of food born bacteria and one strain of yeast were used as the test organism. Acetone extract inhibited growth of the cells of L. plantarum, L. sake and Danmuji film yeast. Growth of the film yeast was drastically inhibited in the concomitant presence of 0.03% extract, while other microbes such as L. faecalis. P. pentosaceus, B. subtilis and E. coil grew by succeeding cultivation for 4 to 8 hours after addition of the extract. Water extract, on contrast to acetone extract, at the concentrations of 0.1~1.5% stimulated the growth of lactic acid bacteria, Culture of L. faecalis and L. sake showed an optical density higher than that of control by 40~50 times. The effect was not so apparent against E. coli, S. aureus and Danmuji film yeast.
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곽희진,계수경,강일준 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.4
이상의 결과로 돼지고기는 비교적 미생물 오염도가 낮아 1 kGy조사로 저온성 미생물을 제외한 오염미생물의 생육을 검출한계 이하로 감소시킬 수 있었으며 모든 오염미생믈의 살균을 위해서는 3kGy의 선량이 요구되었다. 감마선조사와 저장기간에 따른 일반성분 변화의 경우 수분함량에 있어서만이 저장기간에 따라 다소 감소되었으며, pH 및 산도는 감마선 조사에 의한 영향은 없었으나 저장기간이 경과함에 따라 pH는 증가하고 산도는 감소하는 경향을 보였다. 총아미노산 조상에서는 가장 많이 검출된 것이 glutamic acid였고, 감마선 조사에 의한 영향도 매우 미비하였다. 무기질 함량의 경우 주요 무기질 성분으로 potassium, phosphorus, sodium, magnesium 순이었고 감마선 조사에 의한 조성변화는 없었다. Meat is a perishable product since it offers favorable conditions for microbial contamination and multiplication. Besides, undercooked and raw meat has been linked to outbreaks of hemorrhagic diarrhea due to the presence of microbial pathogens. Therefore, this study was conducted to find out the effect of the use of gamma-irradiation in order to improve preservation and eliminate this microorganisms. The initial level of microbial contamination in pork was 1.1×10⁴ CFU/g (aerobic bacteria), 5.3×10³ CFU/g (psychrophile), 3.1×10³ CFU/g (coliforms) and 3.2×10³ CFU/g (Listeria), All kinds of contaminated microorgnisms were sterilized by gamma irradiation at 3 kGy. The proximate composition of pork was not significantly changed by irradiation dose and storage period. The pH was slightly increased during storage period and titratable acidity decreased. However, no significant changes in pH and acidity were observed by gamma irradiation. There was no difference in total amino acid content regardless of irradiation dose and storage period. Glutamic acid was detected at high levels throughout the storage period. The major mineral compositions of pork were potassium, phosphorus, sodium, magnesium. No significant difference in the components of minerals were observed by gamma irradiation.
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감마선조사 닭고기의 미생물학적 및 유전독성학적 안전성 평가
곽희진,정차권,강일준 한국조리과학회 2001 한국식품조리과학회지 Vol.17 No.6
Gamma irradiation (1-10 kGy) was applied to chicken for the evaluation of their microbiological safety and possible genotoxicity. In 3 kGy-irradiated sample, the growth of psychrophile was inhibited about 1.5 log cycles and no cells were recovered in total microbial counts. All kinds of contaminated microorganism were sterilized by 7 kGy-irradiation. Also, irradiation followed by freeze-storage at the same time was very effective in inhibiting bacterial growth. The genotoxicity of 10 kGy-irradiated chicken was evaluated by Salmonella Typhimurium reversion assay and in vivo micronucleus assay using mouse bone marrow cells. The results were negative in the bacterial reversion assay with S. Typhimurium TA98, TA100, TA1535, and TA1539. Clastogenic effects were not shown in vivo mouse micronucleus assay at 10 kGy-dose tested.
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