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공영윤(Young Yun Kong),이관기(Kwan Ki Lee),남상윤(Sang Yun Nam),홍남두(Nam Doo Hong) 한국생약학회 1992 생약학회지 Vol.22 No.4
Phellinus linteus was examined for its anticancer activity using an animal model. Water extract of Phellinus Zinteus was prepared from artificially cultivated mycelia. Neither toxicity nor abnormal changes of hematological ` parameters were observed in the rat given orally with high doses of drug extract for 15 days. ICR mice were transplanted with Sarcoma-180 tumor cells intraperitoneally and drug extract was daily given to the mice from 1 day after tumer transplantation for 3 weeks. Administration of drug extract significantly prolonged the survival duration of Sarcoma 180-transplanted mice. For the better understanding of the anticancer activity, we have examined the effect of the drug extract administration on various killer cell functions, such as natural killer(NK) cells, cytotoxic T-lymphocytes (CTL) and macrophages which have been known to be main effector cells in immune responses against tumors. The results from the 4 hr <sup>51</sup>Cr-release assay have shown that the drug extract augments mouse NK cell activity but neither CTL nor macrophages. It is possible, then, that the anticancer activity of the Phellinus linteus may be associated with augmentation of NK cell function in the cancerated hosts.
Unraveling the Paradoxical Action of Androgens on Muscle Stem Cells
서지윤,김지훈,공영윤 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.2
Androgens act in almost all tissues throughout the lifetime and have important roles in skeletal muscles. The levels of androgens increase during puberty and remain sustained at high levels in adulthood. Because androgens have an anabolic effect on skeletal muscles and muscle stem cells, these increased levels of androgens after puberty should lead to spontaneous muscle hypertrophy and hyperplasia in adulthood. However, the maintenance of muscle volume, myonuclei number per myofiber, and quiescent state of satellite cells in adulthood despite the high levels of androgens produces paradoxical outcomes. Our recent study revealed that the physiological increase of androgens at puberty initiates the transition of muscle stem cells from proliferation to quiescence by the androgen-Mindbomb1-Notch signaling axis. This newly discovered androgen action on skeletal muscles underscores the physiological importance of androgens on muscle homeostasis throughout life. This review will provide an overview of the new androgen action on skeletal muscles and discuss the paradoxical effects of androgens suggested in previous studies.
Mind Bomb-2 Regulates Hippocampus-dependent Memory Formation and Synaptic Plasticity
김소미,김태현,이혜련,공영윤,강봉균 대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6
Notch signaling is a key regulator of neuronal fate during embryonic development, but its function in the adult brain is still largely unknown. Mind bomb-2 (Mib2) is an essential positive regulator of the Notch pathway, which acts in the Notch signal-sending cells. Therefore, genetic deletion of Mib2 in the mouse brain might help understand Notch signaling-mediated cell-cell interactions between neurons and their physiological function. Here we show that deletion of Mib2 in the mouse brain results in impaired hippocampal spatial memory and contextual fear memory. Accordingly, we found impaired hippocampal synaptic plasticity in Mib2 knock-out (KO) mice; however, basal synaptic transmission did not change at the Schaffer collateral-CA1 synapses. Using western blot analysis, we found that the level of cleaved Notch1 was lower in Mib2 KO mice than in wild type (WT) littermates after mild foot shock. Taken together, these data suggest that Mib2 plays a critical role in synaptic plasticity and spatial memory through the Notch signaling pathway.
박인영,Myongsun Son,안은선,Young-Woong Kim,공영윤,윤영대 한국분자세포생물학회 2020 Molecules and cells Vol.43 No.11
Lck-interacting transmembrane adaptor 1 (LIME) has been previously identified as a raft-associated transmembrane protein expressed predominantly in T and B lymphocytes. Although LIME is shown to transduce the immunoreceptor signaling and immunological synapse formation via its tyrosine phosphorylation by Lck, a Src-family kinase, the in vivo function of LIME has remained elusive in the previous studies. Here we report that LIME is preferentially expressed in effector T cells and mediates chemokine-mediated T cell migration. Interestingly, in LIME-/- mice, while T cell receptor stimulation-dependent proliferation, differentiation to effector T cells, cytotoxic T lymphocyte (CTL) function and regulatory T lymphocyte (Treg) function were normal, only T cell-mediated inflammatory response was significantly defective. The reduced inflammation was accompanied by the impaired infiltration of leukocytes and T cells to the inflammatory sites of LIME-/- mice. More specifically, the absence of LIME in effector T cells resulted in the reduced migration and defective morphological polarization in response to inflammatory chemokines such as CCL5 and CXCL10. Consistently, LIME-/- effector T cells were found to be defective in chemokine-mediated activation of Rac1 and Rap1, and dysregulated phosphorylation of Pyk2 and Cas. Taken together, the present findings show that LIME is a critical regulator of inflammatory chemokine-mediated signaling and the subsequent migration of effector T cells to inflammatory sites.
안소희,장은해,최준혁,이혜련,Joseph Bakes,공영윤,강봉균 한국통합생물학회 2013 Animal cells and systems Vol.17 No.3
Fear conditioning has been used to study pathogenic mechanisms underlying anxiety disorders. Several studies have shown that humans with anxiety disorders exhibit strong fear responses during the acquisition of conditioned fear. However, there have been no studies investigating whether basal anxiety within the normal range is related to conditioned fear in rodents. We hypothesized that individual differences in conditioned fear are correlated to the basal anxiety level of each individual. To test this hypothesis, we measured the basal anxiety of mice by using the elevated-plus maze (EPM) and open field test (OFT) and correlated these data with contextual freezing during contextual fear conditioning (CFC). Strong correlation was found between the basal anxiety level measured in the OFT and contextual freezing in the CFC. Baseline freezing was also strongly correlated with the contextual freezing level during the retrieval phase of CFC. However, the basal anxiety level measured in the EPM was correlated neither with conditioned fear nor with baseline freezing in the CFC. These results suggest that both basal anxiety in the OFT and baseline freezing are related to contextually conditioned fear.
당귀의 Coumarin 성분연구 : Decursin의 구조이성체 Decursinol Angelate의 분리 및 정량
류경수(Kyung Soo Ryu),홍남두(Nam Doo Hong),김남재(Nam Jae Kim),공영윤(Young Yun Kong) 한국생약학회 1990 생약학회지 Vol.21 No.1
From the root of Angelica gigas Nakai, decursinol angelate was isolated, which is structural isomer of decursin. Raw material contained average 2.44% of decursinol angelate and 3.30% of decursin by the GC analysis.
권창호(Chang Ho Kwon),홍남두(Nam Doo Hong),김남재(Nam Jae Kim),공영윤(Young Yun Kong),장순욱(Soon Wook Chang) 한국생약학회 1988 생약학회지 Vol.19 No.3
An aromatic hydrocarbon, mp 79∼80°, C<sub>11</sub>H<sub>12</sub>O_5, was first isolated from Phyllostachys sp. and identified as 1, 4-benzenedicarboxylic acid 2`-hydroxyethyl methyl ester. Five compounds isolated after alkali hydrolysis were identified as vanillin, 4-hydroxyacetophenone, terephthalic acid, 4-hydroxybenzaldehyde and friedelin.