DNA Protection by Methylation with Alu I Methylase against Cleavages of Hind III, Sst I, Pvu II and Sac I Endonucleases

강선철,윤호섭,유욱준,Kang, Sun-Chul,Yoon, Ho-Sup,Yoo, O. Joon 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.1

Alu I methylase로 변형시킨 DNA는 Alu I 특정 염기 배열을 내부에 공통적으로 가지고 있는 제한효소들 즉, Hind III, Sst I, Pvu II, Sac I들에 의하여 정상적으로 절단되지 않았다. 이 효소들의 반응 결과를 정량적으로 분석하기 위하여 tritium으로 label된 pBR 322 DNA와 pPG 3282 DNA를 사용하였는데 pBR 322 DNA에는 Hind III와 Pvu II site가 각각 1 개씩, pPG 3282 DNA에는 Sst I과 Sac I site가 각각 1개씩 있는 점이 절단된 후의 분석하기에 용이하기 때문이다. 각각의 경우를 분석한 결과로부터 다음과 같은 결론을 얻을 수 있었다 : (1) Sst I endonuclease가 Alu I methylase에 의해 methylation된 DNA를 느리게라도 절단할 수 있는 점으로 보아 Sst I methylase가 발견된다면 그 specific methylation site는 Alu I methylase의 경우와 달리 internal cytosine이 아닐 것이다. (2) Pvu II와 Sac I endonuclease는 Alu I methylase에 의해 methylation 된 DNA를 전혀 절 단하지 못하는 점으로 보아 Pvu II와 Sac I methylase가 발견된다면 그 specific methylation site는 두 경우 모두 internal cytosine일 가능성이 높다. The cleavage reactions of Hind III, Sst I, Pvu II and Sac I endonucleases were inhibited by the methylation of Alu I methylase. To analyze the cleavage reactions quantitatively, tritium labeled pBR 322 and pPG 3282 plasmid DNAs (pBR 322 DNA contains Hind III and Pvu II sites, while pPG 3282 DNA which is a hog gastrin cDNA clone contains Sst I and Sac I sites) were used, and the degree of the inhibition against cleavages by the four kinds of endonucleases was measured. The results imply that we can predict the specificities of the related hexameric restriction methylases which have not been isolated yet. And the predictions are the followings: (1) The methylation sites of Sst I methylase is not identical with Alu I methylase since Sst I endonuclease slowly cut the sequences methylated by Alu I methylase. (2) The methylation sites of Pvu II and Sac I me thy lases could be identical with Alu I methylase since Pvu II and Sac I endonucleases could not cut the sequences methylated by Alu I methylase.

메타쉐콰이어 추출물의 항균 활성

강선철,비백 바자파이 한국작물학회 2006 한국작물학회 학술발표대회 논문집 Vol.2006 No.-

인산가용화균 Aspergillus sp. PS-104의액침배양중 Pellet 크기에 영향을 주는 요인

강선철,신승용 한국응용생명화학회 2007 Journal of Applied Biological Chemistry (J. Appl. Vol.50 No.1

식중독균에 대한 오메가-3 지방산 EPA의 항균 활성

강선철,김학렬,신승용 한국작물학회 2006 한국작물학회 학술발표대회 논문집 Vol.2006 No.-

Penicillium sp.의 고체배양시 포자형성에 미치는 황토의 영향

강선철,정종배 대구대학교 과학기술연구소 1999 科學技術硏究 Vol.5 No.5

A fungus, Penicillium sp. GL-101, isolated from soil showed the high phosphate-solubilizing activity in potato dextrose broth-rock phosphate to produce free phosphates to the culture broth. In order to make the mass production of the conidia from this fungus, we cultured it on various solid-based media like corn, rice, rice bran, and compost, supplemented either with 4%(w/w) loess or without it. As a result, the fungus highly produced conidia ranging from 3.3 to 5.6 × 10^(9) conidia/g·media on these solid media at 20-days cultivation except compost-based medium, which was 10 times less than others. Addition of loess increased the conidial formation of Penicillium sp. GL-101 in corn-based medium (1.2 ∼ 3.0 times), rice-cooked medium (1.6 ∼ 4.6 times), rice bran-based medium(1.1 ∼ 1.8 times) and compost-based medium (1.7 ∼ 3.5 times), compared to the solid media without loess.

Drug delivery 효과증진을 위한 새로운 cyclodextrin의 생산공정 개발

강선철 대구대학교 공과대학 1998 산학연 공동기술개발컨소시엄 최종보고서 Vol. No.

억새즙액 배지를 이용한 Bacillus Ientimorbus G-74 균주의 배양 최적화

강선철,서해정 한국환경농학회 2004 한국환경농학회지 Vol.23 No.1

Miscanthus purpurascens juice containing potassium (37,952 mg/L), nitrogen (14,000 mg/L), phosphorus (6,800 mg/L), magnesium (5,969 mg/L), calcium (5,910 mg/L), etc., was investigated to develop a novel meidum for the mass cultivation of useful microorganisms. For this research, we first isolated an antagonistic bacterium G-74 from soil, which showed strong growth inhibition against two phytopathogenic fungi, Rhizoctonia solani and Botrytis cinerea, and identified as Bacillus lentimorbus G-74 based on the morp- hological characteristics and MIDI analysis. Culture conditions for G-74 strain in the M. purpurascens juice medium were optimized. Dilution rate of the medium, temperature and initial pH for the optimum growth of G-74 strain were 30% (V/V), 35℃ and 5.0, respectively. It was found that additions of 2.0% (W/V) corn starch as a carbon source and 1.0% (W/V) yeast extract as a nitrogen source in this medium increased B. lentimorbus G-74 growth to 66% more efficient than Luria Bertani medium. 억새즙액을 이용하여 유용한 미생물의 대량배양을 위한 새로운 배지를 개발하고자 본 연구를 수행하였다. 억새즙액의 성분을 조사한 결과 칼륨 (37,952 mg/L), 질소 (14,000 mg/ L), 인산 (6,800 mg/L), 마그네슘 (5,969 mg/L), 칼슘 (5,910 mg /L) 등이 주성분을 차지하였다. 본 연구를 위하여 먼저 식물병원성균인 뿌리썩음병 원인균인 Rhizoctonia solani, 잿빛곰팡이병을 일으키는 Botrytis cinerea에 대하여 강력한 저해 활성을 갖는 길항균 G-74를 토양으로부터 분리하였으며, 이 균의 형태관찰과 MIDI 분석을 통하여 Bacillus lentimorbus G-74 균주로 최종 동정하였다. 이 균의 억새즙액 배지에 대한 최적 배양조건을 조사한 결과 30% (V/V) 희석배지, 35℃ 배양온도, 5.0의 초기 배지 pH에서 가장 좋은 성장을 기록하였다. 또한 이 배지에 탄소원으로서 2.0% (W/V)의 corn starch 및 질소원으로서 1.0% (W/V)의 yeast extract를 추가적으로 첨가하면 최상의 성장을 보여주었으며, 이 경우 대조구인 LB 배지보다도 균체성장이 66% 이상 증가하였다.

항진균물질 KP-3 생산균주의 분리동정 및 항생물질의 정제

강선철,박영구 대구대학교 과학기술연구소 1995 科學技術硏究 Vol.2 No.1

A bacterium producing an antifungal compound KP-3 isolated from soil was selected and identified as Bacillus sp. B-003. The antibiotic substance KP-3 was active against Microsporum gypseum, Trichophyton rubrum and Epidermophyton floccosum at the concentrations of 0.15 - 10.0 ㎍/ml except Candida albicans. This compound was isolated from the fermentation broth (12L) and completely effected by chromatography on silica gel. The Rf value of purified KP-3 (5mg) was 0.91 when TLC plate was developed with methanol - ethyl acetate (1.5, v/v).

제한효소 Hind III , Sst I , Pvu II , Sac I 의 DNA 절단 반응에 있어서 Alu I methylation 의 억제 효과

강선철,윤호섭,유욱준 ( Sun Chul Kang,Ho sup Yoon,Oog . Joon Yoo ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.1

The cleavage reactions of Hind III, Sst I, Pvu II and Sac I endonucleases were inhibited by the methylation of Alu I methylase. To analyze the cleavage reactions quantitatively, tritium labeled pBR 322 and pPG 3282 plasmid DNAs (pBR 322 DNA contains Hind III and Pvu II sites, while pPG 3282 DNA which is a hog gastrin cDNA clone contains Sst I and Sac I sites) were used, and the degree of the inhibition against cleavages by the four kinds of endonucleases was measured. The results imply that we can predict the specificities of the related hexameric restriction methylases which have not been isolated yet. And the predictions are the followings: (1) The methylation sites of Sst I methylase is not identical with Alu I methylase since Sst I endonuclease slowly cut the sequences methylated by Alu I methylase. (2) The methylation sites of Pvu II and Sac I methylases could be identical with ALu I methylase since Pvu II and Sac I endonucleases could not cut the sequences methylated by Alu I methylase.

억새즙액 배지를 이용한 Bacillus lentimorbus G-74 균주의 배양 최적화

강선철,서해정 한국환경농학회 2004 한국환경농학회지 Vol.23 No.1

억새즙액을 이용하여 유용한 미생물의 대량배양을 위한 새로운 배지를 개발하고자 본 연구 를 수행하였다. 억새즙액의 성분을 조사한 결과 칼륨 (37,952 ㎎/L), 질소 (14,000 ㎎/L), 인 산 (6,800 ㎎/L), 마그네슘 (5,969 ㎎/L) 칼슘 (5,910 ㎎/L) 등이 주성분을 차지하였다. 본 연 구를 위하여 먼저 식물 병원성균인 뿌리썩음병 원인균인 Rhizoctonia solaru, 잿빛곰팡이 병 을 일으키는 Botrytis cinerea에 대하여 강력한 저해 활성을 갖는 길항균 G-74를 토양으로 부터 분리하였으며, 이 균의 형태관찰과 MIDI 분석을 통하여 Bacillus lentimorbus G-74 균 주로 최종 동정하였다. 이 균의 억새즙액 배지에 대한 최적 배양조건을 조사한 결과 30%(V/V) 희석배지, 35℃ 배양온도, 5.0의 초기 배지 pH에서 가장 좋은 성장을 기록하였다. EH한 이 배지에 탄소원으로서 2.0% (W/V)의 com starch 및 질소원으로서 1.0% (W/V)의 yeast extract를 추가적으로 첨가하면 최상의 성장을 보여주었으며, 이 경우 대조구인 LB 배지보다도 균체성장이 66% 이상 증가하였다. Miscanthus purpurascens juice containing potassium (37,952 ㎎/L), nitrogen (14,000 ㎎/L), phosphorus (6,800 ㎎/L), magnesium (5,969 ㎎/L), calcium (5,910 ㎎/L), etc, was investigated to develop a novel meidum for the mass cultivation of useful microorganisms. For this research, we first isolated an antagonistic bacterium G74 from soil, which showed strong growth inhibition against two phytopthogenic fungi, Rhizoctonia solani and Botrytis anerea, and identified as Bacillus lentimorbus G74 based on the morphological characteristics and MIDI analysis. Culture conditions for G74 strain in the M. purpurascens juice medium were optimized. Dilution rate of the medium, temperature and initial pH for the optimum growth of G74 strain were 30% (V/V), 35T and 5.0, respectively. It was found that additions of 2.0% (V/V) corn starch as a carbon source and 1.0% (V/V) yeast extract as a nitrogen source in this medium increased lentimorbus G74 growth to 66% more efficient than Luria Bertani medium.