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강근형(Geun Hyung Kang),성충기(Chung Ki Sung) 한국생약학회 1990 생약학회지 Vol.21 No.3
These studies were attempted to investigate the therapeutic effect of Aster yomena extract on the liver damage induced by carbon tetrachloride (CCl₄) in rats. Male rats (SD) were treated with acute concentration of CCl₄, which was 1 ㎖/㎏ of CCl₄ via s.c injection for 3 days or 0.25, 0.5 ㎖/㎏ of CCl₄ via i.p injection. MeOH Ex. administration to rats (500 ㎎/㎏, p.o, twice a day) did not significantly decrease the increment in serum transaminase activities, but is`s frations (Frs), EtOAc and BuOH Fr., administration to rats (300 ㎎/㎏, p.o) significantly (p<0.05) decreased the enzyme activities (GOP, GPT) and liver enlargment. From histopathological observations, we could find that it`s administration prevented the development of fatty liver, necrosis and cytoplasmic vacuolations induced by CCl₄, and it gave a good agreement with the serological data. Futhermore, in doseresponse test, EtOAc Fr. administration at a dose of 400 ㎎/㎏ exhibited the most significant (p<0.01) activity, but it is net dose-dependent. This hepatoprotective effect was observed after 2 days from the last injection of CCl₄. From these results, we could suggest that Aster yomena contains a certain constituent in EtOAc or/and BuOH Fr. related a hepatoprotective activity for CCl₄-induced hepatotoxicity and we are investigating it`s active compound.
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시호 ( Bupleurum falcatum L. ) 의 모상근 배양을 통한 Saponin 생산 - 1. 캘러스 , 부정근 , 모상근과 재배근의 Saponin 양상과 함량 비교
안준철(Jun Cheul Ahn),백윤웅(Yun Woong Paek),성충기(Chung Ki Sung),강근형(Geun Hyung Kang),황백(Baik Hwang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1
In order to survey possibility to produce saikosaponin from in vitro hairy root culture, culture of callus, adventitous root, and hairy root of Bupleurum falcatum L. were estabilished, and quantitative and qualitative aspects in saikosaponin extracted from thses were compared with those of cultivated root. Callus grew well in MS medium containing 0.9 ㎛ 2,4-D. In contrast, both of adventitous root and hairy root grew well in hormone-free MS medium. However, hairy root showed more rapid growth with extensive lateral root branches, characteristics of lower content of water and softer than in adventitous root. Among the selected lines of adventitous root and hairy root were observed difference in the growth rate. Mannopine, one of opine synthesized in the transformed tissue with Agrobacterium rhizogenes A_4 were detected in the extract of hairy root lines. Pattern and content of crude saponin from adventitous and hairy root showed no difference, but somewhat difference from those of cultivated root. However, in callus, distinct production-aspect of saponin was not observed.
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