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갯지렁이, Perinereis aibuhitensis의 재생력을 이용한 양식 산업화
강경호 麗水大學校 産業基術硏究所 1999 産業基術硏究所 論文集 Vol.8 No.-
한국산 갯지렁이류 중에서 산업적으로 가장 중요한 청충의 양식기법 개발에 관한 기초자료를 얻기 위하여 수온별 체중별로 청충의 충체를 prostomium에서 1/4, 2/4 및 3/4부분으로 절단하여 재생상태를 조사한 결과, 절단된 부위에서 pygidium재생이 확인되었다. 수온별 청충의 재생은 13 ±1℃ 구에서 대형개체(2.5 ±0.4g)의 2/4절단구에서 2체절이 재생되었고 소형개체 (1.0 ±0.2g)에서는 3/4절단구에서는 3체절만이 재생됨으로써 저수온구에서는 재생효과가 없었으며 생존율 또한 저조하였다. 한편 20 ±1℃의 고수온 실험구의 경우는 전실험구에서 2~6개의 체절이 증가함으로써 청충의 재생에는 수온이 크게 작용하는 것으로 나타났다. This study was conducted to develop the raising techniques of Perinereis aibuhitensis and focused on regeneration and survival rate. According to the results of experiment on regeneration of P. aibuhitensis, survival rates were very low. However, only at high water temperature, 20℃ or above, they produced the new segments, although the survival rate was low.
Gonadal development and reproduction in the trumpet snail, Charonia sauliae
강경호,김재민,선승천,Liang Liang Zhuo,김현정 한국패류학회 2009 The Korean Journal of Malacology Vol.25 No.1
This study devised and tested a histological staging system for gonadal development in the trumpet snail, Charonia sauliae, which was collected along the south coast of Jeju Island, South Korea. This paper details for the first time the gonadal development characteristics of C. sauliae. Ovary and testis development in C. sauliae can be roughly divided into five stages: growing, mature, spent, degenerative, and resting. The trumpet snail has multiple fecundation and fertilization [cycles?] during the spawning season for out-of-step gonadal development in high-temperature and low-salinity environments. Female trumpet snails tended to have larger shells and were more abundant than males (64.26% of all animals collected were female).
Short Term Storage and Cryopreservation of Trumpet Shell Charonia sauliae Sperm
강경호,손성춘,Bin Zhou 한국패류학회 2008 The Korean Journal of Malacology Vol.24 No.1
With the purpose to estimate the possibility of short-term storage and cryopreservation for sperm of Charonia sauliae, which is a potential preparation for its artificial reproduction and further research, in this study, protocols for short-term storage and cryopreservation of trumpet shell sperm was optimized. The effects of different immobilizing solutions, dilution ratios were estimated for short-term storage. And the effects of different cryoprotectant extenders and freezing rates were estimated for cryopreservation in terms of motility and survival of sperm. The results indicated that the artificial sea water of 350 mOsmol/kg is a better immobilizing solution and sperm which was diluted at a ratio of 1:1 (v/v) had higher motility and survival rate during short-term storage. The effect of 5% dimethyl sulfoxide was significantly better than those of other cryoprotectant extenders. And a freezing rate of -20℃ min-1 showed better effect than other freezing rates. In conclusion, this study optimized some key factors of the short-term and cryopreservation of C. sauliae sperm, which can provide valuable data for germplasm conservation and artificial propagation of C. sauliae.