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      • 大腸菌 培養濾液(Colicin)의 分離와 이것의 Salmonella菌에 對한 發育抑制能

        黃善哲 高麗大學校 倂設 保健大學 保健科學硏究所 1974 保健科學論集 Vol.5 No.1

        As a second part of the antagonistic effect study of E. coli against the certain enteropatho-genic bacteria, Salmonella groups, two tasks have been attempted; the purification of colicin, the E. coli metabolite, by means of the Millipore membrane filtration and the measurement of inhibitory effect of the filtrate against the groups of Salmonella. Some of the findings of the experiments are, hereby, reported as follows: 1. Colonies of Salmonella groups when grown in the mixture of E. coli showed definite decrease in its number compared with that of E. coli. It is evident that E. coli has certain degree of antagonistic effect against some other enteric bacteria. 2. The inhibitory effect of E. coli varied to the groups of Salmonella depending upon the groups in the genera mixed with. However, the degree of the effect could hardly be measurable. 3. The inhibitory effect of E. coli and the dilution factors of the bacterial mixture showed anti-proportional results, where as the best result was obtained at 1 : 1,000 dilution. This also suggested that the adequate preservation of the clinical specimens are very essential. 4. The E. coli bacterial metabolite was purified by means of Seitz filtration and designated as "colicin." The sterility test of the filtrate was successful, and further studied of its inhibitory effect against four groups of Salmonella organisms of which the results are reported as follows; ⅰ) As analogous to the data obtained by the preliminary study. The colicin also has the inhibitory effect against the groups of Salmonella organisms. Further, the clear evidence of the inhibitory effect of the colicin was obtained when the test strains have been diluted in high proportion. ⅱ) However, the colicin failed to show a complete inhibitory effect against the test strains despite the strains have been diluted with the colicin up to 1 : 10,000 dilution. Considering the ability of the colicin to inhibit Salmonella organisms by means of "in vitro" method, the action within the test animals hardly can be estimated with sequential explanation. ⅲ) More organisms suvived in the colicin-Salmonella mixture than in the E, coli-Salmonella mixture at the same dilution. Consequently, the inhibitory effect of the colicin to bacteria are lesser on its degree than that of the cell-cell mixture. ⅳ) The qualitative changes of the bacterial growth in the animal inoculation, decreasing in its colony count could be noticed, though the quantitative measurement of the colicin potentiality within the animal body could not be established.

      • 임상실습을 위한 산·학 연계실태와 그 개선방법에 관한 연구

        선철,김혜순,이창규 高麗大學校 倂設 保健大學 保健科學硏究所 1992 保健科學論集 Vol.18 No.1

        The mandatory clinical practice or internship program was included in the regular curriculum with 10 credit since 1991 school year. Therefore, new clinical practice education will be initiated form spring semter of 1993 for 8 weeks for each student of medical technology. The outcome is the result of the extension of school years from provious 2 years to 3 years starting from 1991. Since the credit-related clinical practice for medical technology student was added to the curriculum as a new teaching program, many problems involved with its processing have been encountered to prepare before sending them out to hospital laboratories. Some major problems among many complicated ones are :1) capacity problem of laboratory facilities to accomodate students who should comply was the curriculum requirement. Compared to the number of students, laboratory facilities are too small in number.2) assignment of clinical practice education coordinator in the laboratory is currently almost infeasible due to budget problem and his or her qualification. It is common practice that education coordinator is an essential condition in accreditation of teaching facilities for the related students. However, it is probable that sencor mediated technologists or superior of the laboratory should lake the responsibility in place of the coordinator for the time being, of course this should be a temporary alternative. There should be a fixed coordinator in the laboratory who is totally eligible to handle the internship program. 10 or are just about the half of total credits established in a semester. Consquently, it is very natural to pay adequat amount of money to the laboratory facilities in return of benefit obtained through the clinical laboratory practice. To calculate the adequate amount of money, it is necessary to figure out the budget of tuition and allocated school laboratory expenses. These problems should be discussed bilaterally between representives of teaching hospitals and colleges, and be prepared in written document all the conditions that are agreed apon.

      • 소아에서 소뇌출혈을 동반한 털모양별세포종 1예

        선철,김범태,이세영,임수빈,신원한 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

        Spontaneous intracranial hemorrage is rare in the childhood, which is usually caused by vascular anomalies, blood dyscrasia and trauma. A 15-year-old child presented with sudden headache while he was studying. The fretures of radiologic images suggested the cerebellar hematoma caused by the underlying lesion such as vascular malformation or cavernous hemangioma. The operation finding revealed that the hematoma was caused by a tumor, which adhered to the superior medullary velum of cerebellum. The pathology revealed it to be hemorrhagic pilocytic astrocytoma. The hemorrhagic pilocytic astrocytoma should be considered in the diagnosis of the lesion in cerebellar hematoma.

      • 임상병리사의 교육현황과 개선방향

        선철,김혜순,윤효숙 高麗大學校 倂設 保健大學 保健科學硏究所 1992 保建科學硏究論集 Vol.1 No.1

        The school-year for Medical Laboratory Science at junior college level was extended from previous two years to three years from 1991. It is considered to be a historical turning point on the education for medical laboratory science. The field of medical technology, including students and laboratory personnel, can be transformed to bring up its standard with the enforced mandatory internship program for a eight-week on the job training and education. 10 credit units are allocated for the program. Education of basic sciences such as fundamental chemistry, physics, biology and computer are added to the curriculum to mobilize the application capacity for the advanced courses. And foreign language courses, beside English, were programed to ease the language barrier for the job at abroad. It is expected to produce better qualified medical technologist taking advantage of school-year extension. However, it is also predicted that there will be unavoidable frictions and frustrations between educators and students. These problems might be further reflected to the medical facilities at where new bred medical technologists are supposed to be engaged in jobs. Mutual coordination of all the involved is undoubtedly necessary to overcome problems occurred during the transit period. The effect of the extended curriculum for the medical laboratory science can be foreseeable at least five years from now on.

      • 腸內細菌의 時間差 混合培養이 보여주는 菌數測定의 比較

        黃善哲 高麗大學校 倂設 保健大學 保健科學硏究所 1973 保健科學論集 Vol.4 No.1

        The study was attempted to see more clear relationship among the enterobacteria, especially between the intestinal normal flora and pathogenic enteric bacteria. It has been known that some intestinal normal flora produce the bacterial metabolites that are harmful to other enteric bacteria. One of the metabolites is known as collicin, the protein fraction, possesses certain degree of inhibitory effect against other bacterial growth. As a preliminary study for a colicin purification, the antagonistic effect of E. coli to groups of Salmonella and Shigella has been studied by means of in virto quantitative culture method. 1. E.coli showed definite inhibitory effect against both Salmonella and Shigella groups in the mixture of two organisms. 2. The inhibitory effect of E.coli in the E.coli-Salmonella and the E.coli-Shigella mixture occurred from 4 hours incubation following the inoculation. 3. Even the complete inhibition of pathogenic enteric bacterial growth was noticed in the E.coli- Salmonella mixture at overnight incubation. 4. Among the diluted mixtures, 1 : 100, 1. : 1,000 and 1 : 10,000, survival rate of pathogenic enteric bacteria in the mixture with E.coli, showed least affected at the 1 : 1,000 dilution. 5. It was found that the antagonistic effect of E.coli against groups of Salmonella-Shigella was depending upon the group of the genera.

      • KCI등재후보

        대한의학회 장애평가 기준

        선철,이경석 대한의사협회 2009 대한의사협회지 Vol.52 No.6

        Several different tools for the evaluation of the physical impairments or disabilities have been employed in Korea. In some cases, the same impairment has received different evaluations in accordance with different evaluation criteria. Sometimes evaluation tools from abroad have been used without efforts of adapting those tools to Korean situations. The general framework for developing impairment evaluation should be objective, useful, and actual. The Korean Academy of Medical Sciences (KAMS) guides has been developed as a tool for the evaluation of impairment by the experts’ discussion and public consensus. The KAMS guides measure the impairment, not the disability, by the rate and encompass all kinds of physical impairments and diseases as many as possible. The guides need the next steps for being legislative and valid from the authorities.

      • 의료개방에 대비한 임상병리검사과학의 발전방향

        선철 高麗大學校 倂設 保健大學 保健科學硏究所 1994 保健科學論集 Vol.20 No.1

        미국의 임상검사과학(Clinical Laboratory Science 혹은 Medical Technology)을 지원하는 학생수가 지난 5년 사이에 무려 30%나 줄어들었다. 특히 Medicare 예산이 삭감되면서 병원수련과정이(hospital based training program)줄어 들었다. 따라서 미국도 이젠 사실상 학교교육(university based program)을 중심으로 한 즉 수익자 부담 원칙에 의한 병리사 교육훈련 형태로 되어가고 있다. 임상검사과학 전공을 기피하는 이유 중에 크게 작용한 것이 AIDS 환자의 급증으로 인한 위험성 그리고 경제침체로 인한 저임금과 24시간 가동에 필요한 불편한 근무 시간대를 들고 있다. 병리사 공급에 비상이 걸린 미국에서는 문제 해결을 위하여 고등학생들을 상대로 순회 recruit 설명회, 전시회 초대 등 안간힘을 쓰고 있다. 아직 우리나라의 임상병리사 수급계획이 미국처럼 전도되지는 않았다 하더라도 대체적인 면에서 개도국의 발전 변화 과정이 선진국의 발자취를 답습 모방하는 형태였다면 우리에게도 미국과 같은 현상이 안오리라는 법 없고 지금도 어느 면에서는 부분적이긴 하나 그런 현상이 나타나고 있어 근무조건을 취업의 전제로 내놓는 경향이 있는 것도 사실이다. 더구나 근무경력, 전공분야, 학력 등이 고려되는 제도가 이루어져 있지 않은 우리나라 임상병리 분야의 앞날은 자기발전이나 이 분야 전체적인 발전을 쉽게 기대할 수 없다. 이러다가는 선진국이 누렸던 임상검사과학의 황금기는 거쳐보지도 못하고 쇠퇴기로 밀려가지나 않나 하는 걱정마저 된다. 임상검사학을 활성화하는 방법으로는 ①학제의 자율화를 통한 자질 향상의 길을 전폭적으로 개방하는 것 ② 면허제도를 개선, 단일 면허를 적어도 우선적으로 1등급과 2등급으로 분류 발급 할 수 있는 제도를 신설할 것 ③면허외에 전공분야별 자격제도를 제정하여 기준에 이르면 전문 병리사가 되어 직업에 대한 자부심과 긍지를 가질 수 있도록 할 것 ④개방화시대에 대비한 자질과 경쟁력을 향상하여 개방물결에 대응이 가능토록 할 것 ⑤임상생리기능검사 등 개발을 요하는 분야에 전력 집중하여 업무영역의 확보 내지 확장에 주력할 것 ⑥임상검사학의 관리, 운영에 관한 국제화추세를 확산시켜 임상병리사의 기능확대를 기할 것 ⑦임상검사학에 필요한 기기, 시약의 국산화를 추진할 수 있는 상설 연구팀을 구성하여 이에 전념할 수 있도록 할 것 ⑧전항의 목표달성을 위하여 산 ․ 학의 협동 체제를 구축 운영할 것 위에 열거한 것들 중 꿈같은 것들도 있으나 그렇다고 그런 사례가 없는 것을 허망하게 나열하는 것은 아니고 언젠가는 이루어져야 할 것 들임에 틀림없으며 어려운 것은 뒤로 미루고 우선 할 수 있는 일부터 지체하지 말고 시작해야 하지 않을까 한다. 시작이 반이라는 평범한 진리를 명심하면서 모두가 우리자신을 위한 것임을 강조한다.

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