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9L 쥐 뇌종양에 있어서 체내 BrdU 표식지표와 체외 BrdU 표식지표의 비교
어환,長島正,松谷雅生,高倉公朋 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.12
The study of in vivo bromodeoxyuridine labeling index(BrdU LI) has become an established method alongside conventional histopathologic study for more complete and reliable evaluation of the biological behavior of tumors. However, in vivo BrdU LI study was limited because of requiring patient selection and patient consent In order to compare in vitro BrdU LI with in vivo one, the authors used 15 rats whose brains were stereotactically implanted with 9L glioma cell suspension, and incorporated BrdU into tumors in vivo or in vitro. In vivo BrdU LI was 18.3± 2.1 % ; in vitro LI of tumors incorporated BrdU within 30 minutes after tumor removal was 11.8± 2.6% ; in vitro LI of tumors incorporated 2 hours after removal was 9.7± 0.8% ; in vitro LI of tumors incorporated 4 hours after removal was 6.5± 3.0%. In vitro LIs were significantly lower than in vivo LI(p<0.05), and in vitro LI decreased gradually as the time of BrdU labeling passed But there were not statistically significant among in vitro LIs. In conclusion, it is essential to incorporate BrdU into tumor cells as soon as possible after tumor removal for in vitro BrdU LI. and in vitro LI is considered to take the place of in vivo LI for the evaluation of proliferating activity of tumor cells.
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