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김종배,이동기 대구산업정보대학 1994 논문집 Vol.8 No.-
After passing through both agricultural and industrial phase, today society, due to technological revolution is becoming highly information oriented. This has resulted in the need for greater amounts of information in all fields of society. In step with this trend, office automation, home automation and factory automation are being promoted extensively. In turn these tendencies have been the main cause of grand scale disasters and the problem of ensuring safety in buildings has manifested itself as a problem for the whole society. However at present in most buildings the fire-fighting facilities and supervisory equipments are usually set-up separately, trailing greatly in areas of safety and efficency. Although there are numerous methods for improving security in office buildings, this study will focus on Intelligent Building and ultimately try to reach the goal of ensuring safety in buildings through interconnecting security systems with intellectualization of buildings. Untill now security systems were usually set-up and operated apart from the central supervision and control department which oversees and regulates the electricity, air conditioning, sanitary facilities and so on. But in Intelligent Buildings central supervisory, control system is introduced in which fire-fighting, crime prevention facilities, electricity, air conditioning, comunications devices and so on are all inter-connected organically, and thus each facility is comprehensively intergrated and interlocked. From the above viewpoint, planning of a system best suited to the requirements which have taken into consideration future usage and expansion on the basis of extensive professional knowledge and experience, must be the preconditions for the fulfillment of Intelligent Building with its highrate safety capabilities.
요로감염환자에서 혈청학적 방법을 이용한 P-pili특이혈중 항체의 조사
이원용,김종배 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.1
비뇨기계 병원성 대장균의 중요한 병원성 인자 중의 하나로 인정되고 있는 p-fimbriae의 subtype의 분포를 확인하기 위하여 요로감염증으로 확진된 환자의 혈청을 이용하여 immunoblotting을 실시하였고, 이와 동시에 효소면역 측정법을 실시하여 p-fimbriae특이 항체 보유를 확인하였다. Immunoblotting 결과 우리나라 요로감염증환자에서 높은 빈도로 확인되는 p-fimbriae subtype의 분포는 F7₁34(56.7%), F7₂28(46.7%), F13 30(50%) 등이 높게 나타났으며, 이와 같은 결과는 효소면역측정법에서도 동일하게 나타났다. 그러나 P-pili를 순수분리하지 않고 whole cell을 이용한 효소면역 측정법은 교차반응 때문에 비뇨기 감염증의 혈청학적인 진단에 적합하지 않는 것으로 나타났다. 또 우리나라의 요로감염 환자에서 항체 양성율이 높은 F7₁, F7₂, F13 만을 혼합하여 항원으로 이용한 요소면역측정법의 특이도와 민감도가 각각 92.6%, 90%로 나타나, 이와 같은 방법을 임상진단에 응용할 수 있은 것으로 판단되었다. Escherichia coli is one of the most common etiological agents in urinary tract infection. An important virulence factor is the adhesive capacity of E.coli to uroepithelial cell, mediated by bacterial fimbriae. The Adhesion property has been regarded as an important virulence determinant in urinary tract infections. A total of 60 patients, who were diagnosed microbiologically as urinary tract infections, were examined by immunoblotting and enzyme-linked immunosorbent assay(ELISA). Uropathogenic E. coli with recombinant plasmid were positive for mannose resistant hemagglutination (MRHA). For identification of p-fimbriae subtype in uropathogenic E. coli, In the immunoblot analysis, specific bands in the range of p-fimbriae molecular weight of 17KD-22KD were identified. For the distribution of p-fimbriae subype in the patient sera, 34/60(56.7%) were positive for F7₁28/60(46.7%) were positive for F7₂and 30/60(50%) were positive for F13 with immunoblotting method. similar trends were observed in the enzyme-linked immunosorbent assay. Relatively good specificity(92.6%) and sensitivity(90%) were found in the ELISA test system using mixed antigens of purified F7₁,F7₂and F13 p-fimbriae, and 60 sera from patients with urinary tract infections. In conclusion The serological tests were convenient method in diagnosis of urinary tract infections. among those ELISA could be recommended in diagnosis of urinary tract infections.