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PHA로 자극된 T cell에서 분비된 미지의 물질이 지니는 Human Neutrophil의 수명연장, Superoxide 및 Leukotriene C₄생산증가 작용효과
허억,양영목 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-
There are several direct and indirect ways in which T cells could enhance the anti-bacterial capabilities of neutrophils. However it is not yet clear out which molecule or cytokine produced by T cells is involved in the phagocytic action and viability sustaining activity of neutrophils. The aim of this study was about a factor, which produced by the phytohaemagglutinin(PHA)-stimulated T cells, may control those neutrophil actions. Human peripheral blood T cells and neutrophils were isolated by Ficoll-paque density sedimentation from heparinized blood of healthy adult donors. The purity of these cells were more than 90%. T cells were stimulated in various dose(0.1-10gg/ml) of PHA for various times of incubation(0-3 days), and then PHA-stimulated T cell conditioned medium was collected in order to find an optimal dose and incubation time for the neutrophil viability. It was found out that 1,ug/ml of PHA in 12 hours incubation was maximal effective condition for the neutrophil sustaining viability. The effects of PHA-stimulated T cell conditioned medium(TCM) on the neutrophils were used for the comparison with PHA-nonstimulated TCM or enriched medium alone. Neutrophil sustaining viability with PHA-stimulated TCM for 24 hours incubation was significantly higher than other groups (80 ± 10 vs 25 ±15 vs 13 ± 9; p <0.01). The superoxide prodution from neutrophils with PHA-stimulated TCM for 24 hours incubation were also significantly higher than other groups(25±4 vs 11±4 vs 7±5; p <0.01). In the leukotriene C4 (LTC4) release, neutrophils with PHA-stimulated TCM for 24 hours incubation were different from other group (105 ± 20 vs 65 ±1O vs 25 ± 32 ; p <0.01), and unlikely other parameters the cells with PHA-nonstimulated TCM was different from the cells with enriched medium alone(65 -1:10 vs 25-± 32; p<0.05). In two dimension electrophoresis it was shown that PHA-stimulated T cells enhanced three proteins(66kD, 60kD, 45kD) and diminished one(40kD) in the production and/or release of proteins in comparison with PHA-nonstimulated T cells. These data suggest that these proteins from the PHA-stimulated T cells might be involved in the phagocytic actions of neutrophils.
Human Neutrophil의 수명연장과 Superoxide 생산에 관여하는 미지의 Monocyte 생성물질
허억,배진우 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-
It has long been known that neutrophils are quickly infilterated and recruited to infected sites and then kill invaders by phagocytic action. Unfortunatly it is not yet revealed which molecule or cytokine is involved in the phagocytic action and viability sustaining activity of neutrophils. The aim of this study was whether lipopolysaccharide (LPS)-stimulated monocyte may control those neutrophil actions. Human peripheral blood monocytes and neutrophils were isolated by Ficollpaque density sedimentation from heparin anti-coagulated blood of healthy adult donors. After preparation of these cells, the purity of both was more than 90%. Monocytes stimulated in various dose(0.1-10pug/ml) of LPS for various times of incubation(0-3 days). and then LPS-stimulated monocyte conditioned medium was collected in order to find an optimal dose and incubation time for the neutrophil viability. It was found out that 3,ug/ml of LPS in 24 hours incubation was maximal effective condition for the activity of neutrophil sustaining viability. Monocyte conditioned medium (MCM) under this condition was used for the comparison with LPS-nonstimulated monocyte conditioned medium or enriched medium alone. When neutrophils were stimulated with each medium for 1-3 days, the activity of neutrophil sustaining viability with MCM was significantly higher than the activity with other medium (in 1 day of culture, 72-1:8 vs 4311:7 vs 17 ±10; p <O. 01). The superoxide production of neutrophil stimulated with MCM for 24 hours incubation was significantly higher than that with other medium under fMLP doses of 0.1-100,uM (p <0.01). Under fMLP l,uM, the superoxide production is predominantly different between them(23.8±2 vs 10.3±3 vs 7.8±1.6). The maximal effective dose of GM-CSF(granulocyte/macrophage colony stimulating factor ; 10pM) enhanced the neutrophil viability in 1 day of culture (50 ± 4%) . In the study to assess whether MCM contains GM-CSF, anti-GM-CSF antibody slightly blocked the MCM-dependent neutrophil viability(73±9 vs 50±12; p <0.07), indicating that MCM might not contain GM-CSF. These data indicate that LPS-stimulated monocyte surely product a factor for the neutrophil sustaining viability and the enhancement of superoxide production, suggesting that a factor is not GMCSF. If more than one factor were producted form LPS-stimulated monocyte, one minor factor might be GM-CSF.
許億,金仁洪,金相喆 최신의학사 1969 最新醫學 Vol.12 No.2
1. The autohors present 3 cases of nearoflfromas involving the formamen magnum and atlas. The pertinent literature is reviewed 2. The most frequently occuring and significant symptoms and neurological signs are reviewed. The outstanding initial symptoms in our cases were posterior cervical pain, suboccipital headache and wealness of an extremity withe peculiar paresthesias. In all cases there were paralysis of upper motor neuron type in one or more extremities and objective sensory disturbance. The protein cotent of S. F. was increased in all cases and cervical myelograms revealed partial or complete block of Pantopaque column The removal of tumors were accomplished by means of a combined cervico-occipial operation, that is, a Subcccipital cruniectorny and an upper cevical laminectorny. After the operation all p 3tents made a satisfactory recovery.
한국산 겨우살이 추출물 M11C(렉틴 구성물질)가단구세포의 TNF-α 유전자 발현유도 및 분비에 비치는 효과
허억 한국약용작물학회 2007 韓國藥用作物學會誌 Vol.15 No.1
It is well-known that Korean mistletoe (Viscum album) extract has an immune activity and anticancer effect. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to examine whether this extract might activate human peripheral monocyte to produce tumor necrosis factor-α (TNF-α). To examine the effect of M11C on the production of TNF-α from monocyte, the monocyte were stimulated by the M11C, and then collected the supernatant (M11C stimulated monocyte-conditioned media; MCM). MCM was treated to the TNF-α sensitive L929 cells, and then L929 cytotoxicity was measured by means of MTT. MCM had cytotoxic effect on L929. And the cytotoxic effect of MCM on L929 was almost abolished by anti-TNF-α antibody. These data indicated that MCM contained TNF-α, suggesting the TNF-α generation from M11C-stimulated monocyte. This suggestion was confirmed from the data that TNF-α was highly detected in MCM by immunoblotting technique. M11C effect on TNF-α production from monocyte was in the dose and stimulating time dependent manners. Also the effect of M11C on the expression of TNF-α mRNA from monocyte was shown in the dose and stimulating time dependent manners. As a result, Korean mistletoe extract, M11C, could be used for an immunostimulator.