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RISS 인기검색어
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- 저자 : 全容赫(Y. H. Chun) (검색결과 3 건)
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Triamcinolone Acetonide에 의한 口蓋裂 발생기전에 관한 조직화학적 관찰
催奎南,全容赫,羅福瑛 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.1
Physiologic levels of corticosteroids along with other hormones and growth factors are known to be essential for normal development of the secondary palate whereas, pharmacologic dose of these steroids specifically disrupt palatal development but still mechanism of their teratogenic action remains controversial and little have been reported on their effect on cell surface materials. Therefore, changes of palatal matrix components and carbohydrate rich cell surface macromolecules during the process of triamcinolone-induced cleft palate in DDY mouse in vivo mere investigated histochemically. Administration of triamcinolone acetonide delayed palatal shelf elevation and changed the shape of shelves to thicker and shorter, when the shelves finally reached horizontally, the distance between shelves was too far to contact each other but under these circumstances, no difference was seen in the palatal tissue histologically including medial edge epithelial degenerative change. PAS positive reactivity was great!:; increased in the shelf epithelia just prior to elevation and diastase sensitive materials were increased both in mesenchyme and epithelia prior to and during fusion and decreased thereafter. Whereas, TAC markedly reduced its reactivity prior to shelf elevation but the reactivity on diastase resistant PAS materials were recovered thereafter which suggest that the fluctuation of PAS positive materials largely depend on morphogenetic status of the palatal shelves. Sulfated acid mucopolysaccharide was increased in mesenchyme trnasiently prior to shelf elevation but T.4C inhibited its increment. With the results, it seems that TAC-induced cleft palate is primarily due to changes in quantity and quality of the palatal matrix and cell surface macromolecules with consequent morphological change of palatal shelves and interference of morphogenetic movement, shelf elevation.
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마우스 상피세포의 일차배양시 배양양상과 최적조건에 관한 연구
김의숙,박선화,전용혁 고려대학교 의과대학 1992 고려대 의대 잡지 Vol.29 No.2
This study was attempted to compare the characteristics of primary culture of mouse epithelial cells and to determine the effective seeding density for confluency on two substrates. The dispersed single epithelial cells were cultured on culture dish coated with type Ⅲ collagen and culture slide. The culture conditions were adjusted to 3 DMEM:1 Fl2 media with 10% FBS, 37℃ temperature. 5% CO₂ air and pH 7.2-7.4 but the seeding density was changed within 1×10⁴-7×10^5 cells / ㎠ substrate. The results were as follows: 1. The characteristics of cultured cells were very similar in the collagen coated dish-used group and the culture slide-used group. The proliferation, stratification, keratinization and desquamation processes were observed like those in vivo. 2. There was a slight tendency to promote attachment. proliferation and differentiation on the collagen coated dish as compared with the culture slide. But it was not statistically significant. 3. The effective seeding density was in the ragne of 4-5×10^5 cells/㎠ substrate in both groups. But the time for cofluency was shortened accordings to rasing the seeding density.
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