Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

Gliotoxin ameliorates TNBS-induced colitis through regulation of NF-kB, and up-regulation of HO-1

김상욱 ( Kim Sang Ug ),( Jay Min Oh ),( Yeon Hwa Kim ),( Jung Moo Hur ),( Kyo Sang Yoo ),( Yong Sung Kim ),( Joo Jin Yeom ),( Tae Hyeon Kim ),( Suck Chei Choi ),( Chang Duk Jun ),( Yong Ho Nah ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-

<Background & aims> During inflammation in colon, cells of the gut mucosa produce or express various inflammatory mediators, including IL-8, TNF-a and IL-1b, and intercellular adhesion molecule 1 (ICAM-1). These cytokines and chemokines have recently been

대호 간척기 토양의 염농도별 밭작물의 염해 평가

이승헌,류순호,설수일,안열,정영상,이상모 江原大學校 附設 環境硏究所 2001 環境硏究 Vol.18 No.-

This study was carried out to obtain the basic data for selecting the applicable crops in reclaimed land during desalinization period. A pot experiment was conducted with 5 different electrical conductivities of the saturated extracts (ECe 1, 3, 9, 14, and 16 dS·m^(-1)) of soils taken from the Dae-Ho reclaimed tidal lands. Eight crops (Chinese cabbage, radish, tomato, red pepper, buckwheat, soybean, sesame, and green perilla) were grown for 37days. Plant height and number of leaves were surveyed on 2 and 4 weeks after seeding, and on harvest time (5 weeks). After harvest, dry weights of harvested crops were measured and soil chemical properties were analyzed. Emergence rates of crops were comparatively high except sesame. For sesame, there was no emergence at ECe over 3 dS·m^(-1). Growth and dry weight decreased significantly as increasing ECe. The ECe which decreased 50% of dry weight index were 14.2 dS·m^(-1) for radish, 11.4 dS·m^(-1) for Chinese cabbage, 10.2 dS·m^(-1) for tomato for red pepper, 8.9 dS·m^(-1) for buckwheat and green perilla, 8.6 dS·m^(-1) for soybean, and 8.9 dS·m^(-1) for tomato. At higher ECe that start the growth inhibition, increasing 1 dS·m^(-1) in ECe, 7.7, 6.5, 5.9, 5.6, 5.2, and 4.9% of dry weight decreased for buckwheat, green perilla, Chinese cabbage, radish, soybean, and tomato (red pepper), respectively. The critical value of ECe for crop survival except sesame was 15.4~23.1 dS·m^(-1).

항암제 AG60이 흰생쥐 위점막 상피세포의 DNA합성에 미치는 영향 : 자기방사법적연구

박경호,안의태,고정식,하상선,한영복,정영신,홍은경,유보림,김상건,이경영,강종구 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

국균세포벽을 모델로 한 새로운 고정화담체의 개발

유은정,김종덕,송상호,임현수,최명락,정규화 여수대학교 1997 論文集 Vol.11 No.2

The purpose of this investigation was to develop a new carrier using chitin and glucan as the model for Aspergillus oryzae cell wall. In case of the ratio of chitin and glucan(3/1), the activity of the immobilized protease had higher than that of model(chitin/glucan=1/3) and carriers were formed gel. In case of soluble chitin and glucan treated with calcium carbonate, there were a 1.8-fold increase in activity of immobilized protease compared with untreated chitin. The scanning electron microscope image showed that a pore size of this carrier had a homogenized pore size and a smooth surface.

모래다짐말뚝의 원심모델링

유남재,정길수,김상진,채승호 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

This paper is results of extensive centrifuge model experiments about design factors influencing the bearing capacity and the settlement behaviors of SCP (Sand Compaction Pile). Centrifuge model tests were carried out changing design factors for SCP method such as replacement area ratio (as= 20, 40, 70%), improvement ratio to footing width (W/B = 1, 2, 3), and amount of fines in sand pile (#200 = 5, 10, 15). Therefore, the effects of these design factors on the bearing capacity and the settlement behavior of SCP were investigated and changes of stress concentratio rato due to such an design factors were also investigated. Centrifuge model testing technique for preparing and installing centrifuge model of sand compaction pile, using freezing them, was also developed. As results of centrifuge model tests, more fines in sand compaction pile increases the bearing capacity of SCP. Optimum improvement ratio to footing width was found to be 2. Values of stress concentration ratio was in the ranges of 1.5 - 3.5. The depth of bulging in sand piles was found in the range of 2.0 - 2.5 times of pile diameter.

BRS 생체정보 분석방법(分析方法)의 정확도(正確度) 확인 연구

유상구,임무열,오상문,유경배,신연호,박병운,이완희 한국정신과학학회 1998 韓國精神科學學會誌 Vol.2 No.2

BRS는 인간의 잠재능력을 적극적으로 계발 · 활용하여 임의의 시료에 대한 미지 (未知)의 생체정보 (Bio-Information)를 편리하게 분석할 수 있는 효과적이고 경제적인 분석장비이다. 그러나 BRS와 관련된 분석방법 자체의 정확도에 대한 연구는 충분하게 이루어지지 않고 있다. 그래서 BRS 분석방법의 정확도를 과학적으로 확인해보기 위해서 본 연구를 진행했다 실험결과, BRS에 의한 생체정보 분석방법 의 정확도는 측정자의 숙련정도에 따라 다르지만 고급 측정자의 경우에는 95% 이상의 높은 확률로 표준시료의 생체정보를 정확하게 분석할 수 있으며, 측정자의 분석능력이 떨어지는 경우에는 여러 명의 측 정자를 대상으로 비교검토(Cross-Check) 방식으로 실험을 보완하면 90% 이상의 정확도를 확보할 수 있었다. BRS is a sort of analysis-instrument which help to estimate some Bio-Information for a sample conveniently and effectively But, for lack of the study to verify the confidence-degree of the Bio-Information analysis method related to BRS, the availability of the method is not known clearly yet, It was the reason that made authors perform this study And It was possible to arrive at the following two conclusions I though the confidence-degree of the Bio-Information analysis method ny BRS was different according to the degree of the expertness of a person using the instrument in the case of the very skillful expert, the confidence-degree for the standard sample used in this study was reached to 95% up 2 In the general case, to improve of the confidence-degree of the Bio-Information analysis method by BRS, the cross-check method by the several measuring person can be introduced.

쉐도우마스크 측정시스템의 분석 소프트웨어 개발

유우식,장성호,김병만 國立金烏工科大學校附設生産技術硏究所 1995 産業技術開發硏究 Vol.11 No.-

Shadow mask is one of the key components which decide the quality of CRT screen. Therefore, the inspection of the exactness of shadow mask forming is important. However, current contact type coordinate measuring machine can only confirm individual measured points. Analysis software for new non-contact shadow mask inspection system which can reduce inspection time and inspection errors rather than current contact type coordinate measuring machine is developed. The developed software can confirm not only individual measured points but also the whole surface profile of the measured shadow mask.

자수용 DNC검증 및 편집 시스템 개발에 관한 연구

유우식,장성호 金烏工科大學校 1993 論文集 Vol.14 No.-

Presented in this paper is a DNC verification and editing system architecture for embroidery manufacturing. Embroidery machines are numerically controlled machines with multi head, but they have not standard RS-232C communication port in general. So embroidery machines receive NC data from reels of 1 in . wide tape containing holes. But punched tape as an input media has many defects. In this paper, we present DNC(Direct Numerical Control) verification system eliminating punched tape. With the proposed system, NC data editing algorithm are also presented.

갓김치 ether 추출물의 발효 과정상의 생리 기능성 변화

유은정,임현수,최명락,송상호 여수대학교 1999 論文集 Vol.14 No.2

일반 김치의 경우 17±1℃에서 2일 사이에 pH가 4.0에 이르는 것에 비해 갓김치의 경우 18℃에서 저장한 경우에 14일 경에 pH가 4.0(Fig.1)에 이르러 일반 김치 보다 저장성이 3~5배 높은 것으로 나타났으며 총균수에서도 비슷한 양상을 나타내었다. 또한 간암세포에 대한 항암성에서 IC30의 농도가 발효기간별 시료 모두 1000㎍이상으로 나타났으며 발효 0일째 시료가 1000㎍에서 50%의 간암세포 사멸성을 나타내서 가장 항암효과가 우수한 것으로 나타났고, 간정상세포에 대해서는 1000㎍ 이상에서도 28% 이하의 사멸성을 나타내서 정상세포에 대해 큰 영향을 미치지 않는 것으로 나타났다. 또한 항산화 효과에 있어서는 초기(0~2일) 발효시 수소공여능이 53%로 나타나서 다른 식품 추출물의 항산화 효과에 비해서는 비교적 높은 효과를 나타내었다. 그러나 발효 14일에는 15%로 감소되어서, ethyl ether에 의해 추출되어 항산화 효과를 나타내었던 물질들이 발효가 진행됨에 따라 감소된 것으로 사료되었다. The anticancer and antioxidative effects of Mustard Leaf Kimch ether extracts according to various fermentation period were investigated. pH of the Mustard Leaf Kimchi was reached at 4.0 on 14th day, so the period reaching well-fermentating state was up to 3~5 times longer than that of ordinary Kimchi. The anticancer effect on HepG2 by the addition of fermented Mustard Leaf Kimchi ether extracts was higher than that on CCL-13. IC30 of Mustard Leaf Kimchi in HepG2 was showed more than 1000㎍.Especially, 50% of death ratio was showed in HepG2 cells by the addition of ether extracts(0 day). the other side. 28% of death ratio was shown in CCL-13 up to 1000㎍ of ether extracts. As well as the Mustard Leaf Kimchi ether extracts(0-2 days) showed higher antioxidative effect than of others. The hydrogen donor activity was 53% at initial fermentation period, but it was decreased at 14% on 14th day. So, ether soluble antioxidative materials in Mustard Leaf Kimchi was decreased during fermentation.