Primary cell culture isolated human skin

( Jimyung Seo ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1

FCP 12 : The role of bone scintigraphy in Behcet`s disease from the view of the dermatologist

( Jimyung Seo ),( Zhenlong Zheng ),( Dongsik Bang ),( Do Young Kim ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Behcet’s disease(BD) is a multisystemic inflammatory disease with articular involvement(9%-70% in previous reports). However, non-specific arthralgia is also common in BD patients, and the exact diagnosis of joint involvement is often challenging especially for non- rhuematologists. Objectives: To evaluate the usefulness of 99mTc- methylenediphosphonate(Tc-99m-MDP) bone scintigraphy from the viewpoint of the dermatologist Methods: A total of 211 patients were analyzed. Agreement between bone scintigraphy findings and clinical symptoms was assessed with the Cohen’s kappa statistic. At the same time, 104 patients who showed the accordance between imaging studies and clinical symptoms were re-evaluated by rheumatologists. Results: The total kappa value of 211 cases was 0.604, and the agreement on clinical symptom and scintigraphy image was fair. Individual analysis of the 11 categorized jointsshowed that there were statistically significant correlations in wrist(κ = 0.677), shoulder(κ = 0.661), and foot joints(κ = 0.618). Of the 104 cases referred to the rheumatologist, 95 cases(91.34%) were confirmed as articular involvements of BD. The joint involvement of 9 cases diagnosed as other disorders was noticed in hip, spine and knee joints, all of which were the joints showing low correlation of kappa value. Conclusion: Bone scintigraphy can be a simple and useful option to determine the joint involvement of BD for the dermatologist, especially in certain anatomical sites.

Rare sarcomas of the skin: a case series

( Jimyung Seo ),( Yeongjoo Oh ),( Jihee Kim ),( Jun Young Kim ),( Zhenlong Zheng ),( Kyoung Ae Nam ),( Kee Yang Chung ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

All sarcomas of deep soft tissues may also arise in superficial locations and there can be important clinicopathologic differences. Recently, we have experienced some kinds of these rare malignancies occurring in the skin. We report herein the four cases of different sarcomas having a primary cutaneous location: Ewing sarcoma, histiocytic sarcoma, myxofibrosarcoma, and liposarcoma. Extensive metastatic work-up, including a total body computed tomographic and positron emission scan (PET/CT scan), showed the neoplasm to be a primary sarcoma of the skin. All four patients presented with negative sentinel lymph node biopsy specimen and were treated by Mohs micrographic surgery (MMS) using paraffin-embedded sections, i.e. slow Mohs. We hope that our experience will broaden dermatologists’ understanding of these rare sarcomas arising from the skin.

Postoperative treatment of linear thyroidectomy scars by pinhole method using a carbon dioxide laser

( Jimyung Seo ),( Min Seok Lee ),( Howard Chu ),( Jae Won Lee ),( Do Young Kim ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Concerns about scarring are increasing in modern-day society, and various types of lasers have been used to improve the appearance of scars. The site of an open thyroidectomy is the anterior neck, a prominently exposed part of the body, where post-operative scarring can cause patients distress. We introduce the pinhole method using a 10,600-nm carbon dioxide (CO2) laser for the treatment of linear thyroidectomy scars. Five Korean women with linear thyroidectomy scars were included in this study. 2 to 3 weeks after surgery, we performed three sessions of CO2 laser treatments in intervals of 4 to 8weeks for each case. Treatment was performed in ultrapulse wave mode, with a spot diameter of 1 mm, a pulse duration of 0.2 ms, and peak power of 800 W. Mean Vancouver Scar Scale (VSS) scores were statistically significantly lower after laser treatment. Two blinded physicians also evaluated the final scars using a quartile grading scale 3 to 6 months after surgery. Four of five patients showed clinical improvement of more than grade 3 (marked improvement, 51-75%). No significant adverse events occurred. The pinhole method using CO2 lasers is effective and safe for linear thyroidectomy scars. In addition, because CO2 lasers are usually available in most dermatology clinics, this method can be an easily accessible treatment option for dermatologists.

Cutaneous ulceration after injection of interferon alpha in a melanoma patient

( Jimyung Seo ),( Jihee Kim ),( Kyoung Ae Nam ),( Byung Ho Oh ),( Kee Yang Chung ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

A 47-year-old male with high-risk cutaneous melanoma diagnosed in 2013 was treated with the high dose interferon-alpha regimen (20 MU/m2) after radical resection of the primary melanoma. Interferon alfa-2b was subcutaneously injected. Three months later, a painful, erythematous, indurated plaque initially developed at the site of injection on his lower abdomen. Results of skin cultures were negative. Examination of a skin biopsy specimen showed epidermal necrosis and acute inflammatory cell infiltrates in the dermis and subcutaneous tissue, consistent with nonspecific ulceration. Ulcer treatment included local wound care measures, surgical debridement, and change of injection site. The ulcer completely healed in two months. This patient represents a rarely reported case of cutaneous ulcer caused by interferon alpha. Increased awareness of the possibility of cutaneous necrosis after interferon therapy is necessary for early diagnosis to prevent continuation of skin ulcers.

LETTER TO THE EDITOR : Mucocutaneous Telangiectasia as a Diagnostic Clue of Hereditary Hemorrhagic Telangiectasia: An Activin Receptor-Like Kinase-1 Mutation in a Korean Patient

( Jimyung Seo ),( Howard Chu ),( Jin Sung Lee ),( Do Young Kim ) 대한피부과학회 2016 Annals of Dermatology Vol.28 No.2

FCT 1-5 Ingenol mebutate in the treatment of melanoma

( Jimyung Seo ),( Young In Lee ),( Kyoung Ae Nam ),( Jihee Kim ),( Jun Young Kim ),( Mi Ryung Roh ),( Kee Yang Chung ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2

Background: Noninvasive, tissue-sparing, topical treatments may be a highly desirable option in the treatment of melanoma. A number of topical drugs are used off-label or are under investigation. Objectives: We report our treatment experience with ingenol mebutate in patients with melanoma, and evaluate the efficacy and safety of this treatment as a monotherapy or neoadjuvant treatment. Methods: This is a retrospective study on patients with melanomas who underwent topical treatment with ingenol mebutate. Results: A total of five patients (five female; median age, 67 years; age range, 44.87 years) were identified from our search. Considering the anatomically challenging locations and the morbidity of surgery, ingenol mebutate was administered as a monotherapy in two patients with melanoma in situ. The remaining three patients were treated with topical ingenol mebutate before surgery. Of the five patients, only one patient, who had melanoma in situ on the vaginal mucosa and underwent slow Mohs micrographic surgery, presented local recurrence during the follow-up period (13.8 ± 2.8 months). Conclusion: Despite limitations arising from the small number of patients analyzed and the retrospective design, the results of this study provide additional insights into the role of ingenol mebutate in the treatment of in situ melanoma or melanoma on an anatomically challenging location. Further studies are needed to establish the prerequisites that can ensure treatment success.

P128 : Mohs micrographic surgery for extramammary Paget`s disease: a study of 60 Asian patients

( Jimyung Seo ),( Hee Su Kim ),( Ji Hee Kim ),( Hong Sun Jang ),( Byung Ho Oh ),( Mi Ryung Roh ),( Kee Yang Chung ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Extramammary Paget disease (EMPD) is an uncommon intraepithelial tumor characterized by ill-defined margins and high recurrence rates after wide local excision. Objectives: To evaluate the efficacy of MMS for EMPD and establish surgical treatment guidelines for EMPD in Asian patients. Methods: The results of 60 patients with EMPD treated by MMS from 2000 to 2013 have been analyzed retrospectively. Results: There were 54 male and 60 female patients and most were treated by slow-MMS using permanent paraffin-embedded sections. Among 60 patients, 58 had genital lesions and 2 had axillary lesions. The average follow-up period was 61.5 months (14 -140 months). The recurrence rate of EMPD after MMS was 3.3% (n=2) and the mean time to recurrence was 11 months. Among the recurred patients, one had been previously treated by topical 5% imiquimod and the other had undergone prior wide local excision. Estimated 5-year tumor-free rate using Kaplan-Meier graph was 96.3% in all patients for MMS. None of the primarily occurring cases recurred, indicating 0% (n=50) recurrence rate. However, among patients who underwent MMS after initial recurrence, the recurrence rate was significantly higher at 20% (n=10). Conclusion: Our results demonstrate that the recurrence rate of EMPD is low (3.3%) after MMS. MMS is an effective surgical method with low recurrence rate, and can be suggested as the first-line treatment for non-metastatic primary EMPD patients and also for patients with recurred disease.

P037 : Cutaneous serratia marcescens infections in Korea: a retrospective analysis of 13 patients

( Jimyung Seo ),( Dongyun Shin ),( Sang Ho Oh ),( Min Geol Lee ),( Dae Suk Kim ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Serratia marcescens is a gram-negative bacillus belonging to the Enterobacteriaceae family. Because of reported antimicrobial resistance and increasing reports, this strain has received considerable attention and emerged as an important pathogen. Objectives: To reveal clinical and microbiological characteristics of S. marsescens cutaneous infetions Methods: A total of 17 strains from 13 patients, isolated from wound swabs between Nov. 2005 and Mar. 2014, were analyzed retrospectively. Results: Most common infections presented on lower legs, clinically showing painful ulcers (n = 5, 38.4%). The routeof infection was noticed in preexisting skin disorders in immunocompromised patients (5/13), preceding cancer surgeries (2/13), plastic surgeries with filer injection (2/13), medical procedures following cutaneous abscess (2/13), and traumas (2/13). The average treatment duration was 25 days (range from 14 to 42). The isolates showed resistance to ampicillin (16/17), amoxycillin-clavulanic acid (16/17), cefazolin (17/17), cefoxitin (13/17), and tetracycline (11/17). All isolates remained susceptible to 3rd and 4th generation cephalosporins, quinolones and carbapenems. Clinically, among 13 patients, oral quinolone monotherapy were the most preferred antibiotic regimen showing good clinical improvement. Conclusion: This case highlights S. marcescens infections in skin, which call for broad antibiotic coverage due to multiple drug resistance.

Regulation of Hippo signaling by actin remodeling

( Jimyung Seo ),( Joon Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.3

The Hippo signaling pathway controls nuclear accumulation and stability of the transcriptional coregulator YAP and its paralog TAZ. The activity of Hippo-YAP signaling is influenced not only by biochemical signals, but also by cell shape and mechanical tension transmitted through cell-cell junctions and cell-matrix adhesions. Data accumulated thus far indicates that the actin cytoskeleton is a key mediator of the regulation of Hippo-YAP signaling by means of a variety of biochemical and mechanical cues. In this review, we have outlined the role of actin dynamics and actin-associated proteins in the regulation of Hippo-YAP signaling. In addition, we discuss actinmediated regulation of YAP/TAZ activity independent of the core Hippo kinases MST and LATS. Although our understanding of the link between Hippo-YAP signaling and the actin cytoskeleton is progressing rapidly, many open questions remain. [BMB Reports 2018; 51(3): 151-156]