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Eman Mumtaz El Mahdy,Maha Gamal,Basma Emad Aboulhoda,Basent Adel Al Dreny,Ashraf Shamaa,Laila Rashed,Ahmed Nour Eldine Abdallah,Asmaa M. Shamseldeen 한국통합생물학회 2023 Animal cells and systems Vol.27 No.1
Parkinson’s disease (PD), the second common neurodegenerative disease, is characterized by theloss of the dopaminergic neurons in the substantia nigra (SN). Because of L-dopa’s side effects, newtherapeutic methods were considered such as stem cells for PD treatment. The major challengefacing stem cell therapy following transplantations is their peripheral sequestration anddecreased survival. Thus, the ability of erythropoietin (EPO) and low-level laser therapy (LLLT) toenhance mesenchymal stem cell (MSC) proliferation and therapeutic efficiency for alleviatingrotenone-induced PD was investigated. Therefore, we compared the influence of stem cellpreconditioning with erythropoietin versus LLLT on MSCs’ homing ability and the chances ofsurvival. Forty-eight male Swiss mice were included in the in vivo protocol and ten in the invitro work. The mice in the in vivo study were divided randomly into six groups (8 in each);group I; control group and group II; induced PD (untreated) group, group III; L-dopa, group IV;MSCs, group V; EPO-activated MSCs and group VI; laser-activated MSCs. Treatment with MSCs(either preconditioned or not) improved these neurological features by restoring the normalbalance between glutamate and GABA neurotransmitters, and dopamine as well as increasedtyrosine hydroxylase levels. Stem cell therapy decreased oxidative stress, miRNA-155 levels,enhanced neuronal architecture in SN, and decreased the number of apoptotic cells. LLLTenhanced MSC expression of integrin β1 which was reflected in the homing of PKH26-labelledMSCs into corpus striatum and SN. Thus, the optimum results were achieved with laseractivatedMSCs.