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RISS 인기검색어
- 검색키워드
- 주제어 : Substantia gelatinosa neuron (검색결과 7 건)
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마우스 삼차신경 척수로핵 미측소핵 아교질 신경세포에서 Bicuculline Methiodide (BMI)의 비 GABAA 수용체 매개성 반응
최순정 전북대학교 치의학대학원 2010 국내박사
Bicuculline is one of the most commonly used type A gamma aminobutyric acid (GABAA receptor antagonists in electrophysiological research. Because of its poor water solubility, bicuculline quaternary ammonium salts such as bicuculline methiodide (BMI) and bicuculline methbromide (BMB) are preferred. However, a number of studies have shown that BMI has non-GABAA receptor mediated effects. The substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) is implicated in the processing of nociceptive signaling. However, little is known about non-GABAergic action of BMI on the SG neurons of Vc in mice. This study investigated whether BMI has a non-GABA receptor mediated activity on SG neurons of Vc in mice using a whole cell patch clamp technique. The majority of SG neurons tested were depolarized by application of BMI (20 μM) in condition of a high Cl- pipette solution (n=36/54, 66.7%). GABA (30-100 μM) also induced membrane depolarization of SG neuron. Although BMI is known to be a GABAA receptor antagonist, GABA-induced membrane depolarization was enhanced by co-application with BMI. However, free base bicuculline (fBIC) and picrotoxin (PIC), a GABAA and GABAC receptor antagonist, blocked the GABA-induced response. Furthermore, BMI-induced membrane depolarization persisted in the presence of PIC or an antagonist mixture consisting of tetrodotoxin (TTX, Na+ channel blocker), AP-5 (NMDA receptor antagonist), CNQX (non-NMDA glutamate receptor antagonist), and strychnine (glycine receptor antagonist). In addition, BMI induced inward current in voltage clamp mode, however BMB (bicuculline methbromide) and BMC (bicuculline methchloride), another water-soluble derivative of bicuculline are induced outward currents on SG neurons of Vc in mice at dose dependent manner. These data demonstrate that BMI has non-GABAA receptor mediated action and suggest that the non-GABAA receptor mediated action can be mediated by iodide. Further studies are needed to investigate the precise action mechanism underlying the BMI-induced non-GABAA receptor mediated action on the SG neurons of Vc in mice.
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사춘기전 마우스 미측소핵의 아교질 신경세포에서 시트랄의 억제성 신경전달 조절에 대한 연구
Thao, Nguyen Thi Phuong 전북대학교 일반대학원 2020 국내석사
Citral (3,7-dimethyl-2,6-octadienal) is a monoterpene aldehyde consisting of isomers geranial and neral chemically. The substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) is admitted as a key site of receiving and regulating orofacial nociceptive inputs. Although citral is known to be involved in antinociception, the action mechanism of citral on the SG neurons of the Vc has not been fully understood yet. In this study, I examined the direct membrane effects of citral and how citral mediates responses on the SG neurons of the Vc in juvenile mice using a whole-cell patch-clamp technique. Under high chloride pipette solution, citral showed repeatable inward currents that persisted in the presence of tetrodotoxin, a voltage-gated Na+ channel blocker, and 6-cyano-7-nitro-quinoxaline-2,3-dione, a non-N-methyl D-aspartate (NMDA) glutamate receptor antagonist, 2-amino-5-phosphonopentanoic acid, an NMDA receptor antagonist. However, the citral-induced inward currents were partially blocked by picrotoxin, a gamma-aminobutyric acid (GABA) type A receptor antagonist, or by strychnine, a glycine receptor antagonist. Further, the citral-induced responses were almost blocked by picrotoxin with strychnine. I also found that citral exhibited additive effect with GABA-induced inward currents and glycine-induced inward currents were potentiated by citral. In addition, citral suppressed the firing activities by positive current injection on the SG neurons of the Vc. Taken together, these results indicate that citral has glycine- and/or GABA-mimetic actions and suggest that citral might be a potential target for orofacial pain modulation by the activation of inhibitory neurotransmission in the SG area of the Vc. keywords : Citral, GABAA receptor, Glycine receptor, Patch-clamp, Substantia gelatinosa neuron
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양은주 전북대학교 일반대학원 2013 국내석사
The spinal trigeminal nucleus is associated with transmission of orofacial sensory information, and is composed of three subnuclei (oralis, interpolaris, and caudalis). Nociceptive input from the orofacial region is initially processed in the trigeminal subnucleus caudalis (Vc). Especially, the substantia gelatinosa (SG, lamina II) of the Vc is a key element for modulating nociceptive transmission from the periphery to the central nervous system (CNS). Serotonin, 5-hydroxytryptamine (5-HT), influences diverse physiological functions such as sleep, cognition, sensory perception, motor activity, temperature regulation, appetite, hormone secretion, and nociception. At least, 14 subtypes of 5-HT receptors have been characterized and grouped into seven principal classes, from 5-HT1 to 5-HT7. 5-HT7 receptor, one of the most recently identified subtypes, is coupled to adenylate cyclase through the stimulatory Gs protein, and plays physiological roles such as circadian rhythm regulation, thermoregulation, and neuroendocrine regulation in the central nervous system. However, few studies have been conducted to demonstrate whether 5-HT7 receptors are involved in orofacial pain modulation. So, in this study, functional expression of 5-HT7 receptor on SG neurons of the Vc, which are involved in orofacial pain modulation was investigated using perforated patch clamp technique. The bath application of 30 M 8-OH-DPAT, 5-HT1A/7 agonist, induced membrane depolarization, no response, or hyperpolarization in SG neurons of the Vc. The majority of neurons tested were depolarizd in PND (postnatal days) 5-21 and PND 22-84, and 8-OH-DPAT induced different level of membrane potential changes in SG neurons of the Vc in both groups. Further, 8-OH-DPAT-induced depolarization was reversible and reproducible, indicating that the 5-HT receptors involved in 8-OH-DPAT-mediated depolarization was not desensitized in SG neuron by repeated stimulation. Moreover, the study about the working site of 8-OH-DPAT proved that 8-OH-DPAT acts on postsynaptic SG neurons, directly. In addition, 8-OH-DPAT-induced depolarization remained unaltered in the presence of WAY 100635, a selective 5-HT1A antagonist, but was largely blocked by SB 269970, a specific 5-HT7 antagonist. Interestingly, 8-OH-DPAT-induced non depolarization was suppressed by WAY 100635, and some of these neurons were instead depolarized to 8-OH-DPAT plus WAY 100635 pretreatment. These results suggest that the depolarization was mediated via 5-HT7 receptor, but not via 5-HT1A receptor. Finally, 5-HT7 receptor mRNA was detected in SG neurons of the Vc using single cell RT-PCR technique. Taken together, 5-HT7 receptors functionally exist in SG neurons, and SG neurons were depolarized by activation of 5-HT7 receptor in the Vc. In conclusion, this study suggests that the functional involvement of 5-HT7 receptors in descending serotonergic modulation over orofacial pain.
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