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RISS 인기검색어
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- 주제어 : 흉선[胸腺] (검색결과 2 건)
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Cyclophosphamide 투여로 인해 유발된 흰쥐 가슴샘 퇴축 후 재생과정에서 expressed sequence tag 분석
The thymus is the central lymphoid organ for development of bone marrow-derived precursor cells into mature T-cells. It would be very important to understand the molecular mechanism of thymic involution and regeneration to develop methods to normalize or improve host immunity from the decreased immune function caused by thymic involution. The expressed sequence tag (EST) approach is an effective way of characterizing genes expressed in thymus during regeneration since ESTs provide a rapid and reliable method for gene discovery as well as a resource for the large-scale analysis of gene expression of known and unknown genes. In this study, the regenerating thymus cDNA library was constructed in the rat from a model of thymic involution and regeneration induced by cyclophosphamide (150 ㎎/㎏). ESTs were obtained by partial sequencing of 700 randomly selected insert-containing clones. A total of 630 ESTs were analyzed, of which 486 ESTs (78%) matched to known genes and 125 ESTs (19%) matched to other ESTs (unknown genes). The 19 ESTs (3%) did not match with any known sequences. The ESTs were grouped into six main functional categories: matabolism (44%), signaling components (20%), membrane transport (7%), cytoskeleton (2%), cell division (2%) and defence (2%). As a results of RT-PCR analysis, expression of two putative genes (putative gene 01 and E2IG2), musculin, osteoactivin and IL-17A significantly increased in rat thymus during regeneration. The putative gene 01 showed complete homology with ribosomal protein S4 by homology search and multiple alignment of amino acid. These results provide the extensive molecular information on thymus regeneration and will be useful source to identify various genes which may play an important role in the thymus regeneration as well as to clone novel genes. Furthermore, the availability of these data will serve as a basic for further research to understand the molecular mechanism of thymus regeneration.
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