人工降雨下에서 粘土含量에 따른 土壤浸蝕樣相

오세진 건국대학교 1989 국내석사

粒狀硅酸質肥料의 處理에 따른 水稻 生育 및 米質에 대한 效果

洪柱華 忠南大學校 大學院 2005 국내석사

This study was conducted to investigate on improving effects of growth and nutrient uptake of plant and rice quality by treatment of granular silicate fertilizer(GSF) during rice plant cultivation(for 1 year). The place on rice culture test was at the Sungbok-Dong in Nonsan city. Then the chemical properties of the early paddy soil used for culture test, these results were pH 5.9, O.M. 13.7 g kg^(-1), avail. P₂O^(5) 48.9 mg kg^(-1), and avail. SiO₂ 79.8 mg kg^(-1). And the amounts of GSF applied in the paddy soil were 0, 76, 185, 229, 413 and 489 kg 10a^(-1) for the control, S-100, S-130, S-160, S-190 and for S-210 treatment respectively. The results for the this study obtained as follow; At the effectiveness if growth on rice plant, the leaf length and tiller number were more remarkable value at treatments of granular silicate applied than control. The leaf length at the S-100 treatment was the highest as 99.4 cm and the tiller number was the highest as 18.9 no. EA^(-1) at the S-130 treatment. And at the results of plant yield parameters, a grain and straw yield of rice were showed that all of the GSF application treatments were more predominant level than at the control, especially their levels were the highest values as 841.5 and 815.2 kg 10a^(-1) at the S-160 treatment respectively. Also, at the nutritional uptake results of nitrogen(N), phosphate(P₂O^(5)) and silicate(SiO₂) by rice plant after GSF application during the cultivation, the nitrogen and phosphate uptake contents were decreased by increasing in amount of granular silicate, however silicate uptake content was increased at the same applications condition. And then the highest treatment on the silicate uptake content was the S-210 plot as a total amount 85 kg 10a^(-1). In other hand, the efficiency of silicate on the rice plant was the lowest percent as 2.8 % at the S-210 plot. By the way these results have had tendency to appear as positive(interdependency) or negative(competition) effects throughout relation with increasing of the GSF application. Also at the estimated results of quality parameters on rice grains, a perfect kernel in applied GSF treatments was more increased than at the control, but a broken kernel rate was inversely decreased. And in results of theses aspects, the chemical properties of white rices and the taste of rices by the Toyo MA-90A(Toyo rice quality taster) instrument, the amylose content was the lowest value as 18.7 %(generally about 20 %) at the S-160 treatment. Also, the Mg K^(-1) ratio and the cooked rice taste value were the highest level as 0.58 and 69.1 at the S-190 and S-160 treatments respectively. Finally the harvest yields of rice at the S-160 and S-190 plots from these growing factors were more produced about 8∼13 % than control plot. In results of chemical properties in the cultivated soils of all treatments, pH levels in these soils at early cultivation period were considerably increased due to increasing of alkaline cations as Ca, Mg, and K etc. in soil by the GSF application. But this phenomenon of soil pH was resemblingly decreased as original soil according to passing of cultivation period. And the available phosphate(P₂O^(5)) contents were slightly decreased during the all growing stage of rice plant, however the available silicate(SiO₂) contents were increased to depending on increasing of the GSF application. The reason of this increased result was due to increasing of accumulated Si content, and then the target Si concentration levels in rice culture soils for the this study have appeared higher value than the calculated target Si concentration levels. That was reason due to the difference of available SiO₂ content of the Wollastonite and the GSF materials by extraction with 0.5 N HCl. Therefore, the GSF application for the rice cultivation from these results should be expected to obtain the positive effects as enhancement of rice harvest yield and improvement of quality on the cooked rice taste. So the amount of GSF application could be recommended as around 200 mg kg^(-1) for optimal and economical rice cultivation.

제주 토양중에서 분리된 Aspergilli에 대한 분류학적 연구

강상건 제주대학교 대학원 1987 국내석사

Caffein이 Rhodotorula glutinis의 지질대사에 미치는 영향

최경식 건국대학교 대학원 1992 국내석사

Caffeine이 Yeast의 생육 및 지방 대사에 미치는 영향을 조사하고자 caffeine을 0%, 0.01%, 0.05%, 0.10% 첨가한 Glucose 배지에 Rhodotorula glutinis를 접종하여 8일간 진탕 배양하면서 지질 생성량, 증식도, pH, 당 소비율, ATP : citrate layse의 활성을 조사하였다. 1. 증식도는 caffeine 첨가량 증가순으로 감소되어 0.10% 첨가구가 가장 낮았다. 2. pH는 배양 1일째 모든 시험구에서 급격한 변화가 있었으며 배양 기간 동안 점차 낮아지는 경향을 보였으나, 0.10% 첨가구는 다른 시험구보다 높은 pH를 보였다. 3. 당 소비율은 모든 시험구가 대조구보다 작았으나 0.01%와 0.05% 첨가구는 비슷하였으며, 0.10% 첨가구는 대조구보다 현저히 낮았다. 4. ATP : citrate lyase의 활성은 첨가구 모두 배양 4일째까지 대조구에 비해 낮았으나, 0.01% 첨가구의 5일째 활성은 대조구의 최고 활성보다 1.2배 증가하였다. 5. 균체 지질 함량은 대조구에 비해 0.01% 첨가구가 1.1배로 증가하였으나, 0.05% 첨가구는 0.76배의 작은 함량을 보였다. 6. Caffeine 0.01% 첨가구는 생육도와 당 소비율은 대조구에 비해 낮았으나, 효소 활성 및 지질 생성량은 높았다. This study was carried out to investigate the effect of caffeine on the lipid contents, growth rate, pH, sugar consuiption rate and ATP:citrate lyase activity of Rhodotorula glutinis. 1. Cell growth of Rhodotorula glutinis decreased in the media containing 0.10% caffeine than that of control group at 26℃ for 8 days incubation. 2. pH change was remarkably decreased in early incubation time and continuously decreased in all incubation time, but 0.10% caffeine containing group, it was higher than other groups. 3. Sugar consumption rate decreased in the order of addition of caffeine. 4. ATP: Citrate lyase activity decreased until 4 days incubation time but the activity of 0.01% caffeine containing group was increased 1.2 times than that of control group at 5 days incubation respectively. 5. Lipid contents of 0.01% caffeine containing group increased 1.1 times than that of control group respectively. 6. Cell growth and sugar consumption rate of 0.0l% caffeine containing group decreased but lipid content are increased compared with that of control group.

화산회토 감귤원 토양에서 경작년수에 따른 Cu 및 Zn의 함량변화

오명협 濟州大學校 大學院 1998 국내석사

Cu와 Zn을 함유한 부산물 비료, 농약, 화학비료 등을 많이 사용하는 제주도 남제주군 지역 감귤원의 표토와 심토를 경작년수별로 채취하여 경작년수에 따른 0.1M HCl 침출 Cu와 Zn의 함량 변화 및 연속침출법으로 측정한 각 형태별 함량의 변화 및 주요 분포 형태를 조사하였다. 비경작지 표토와 심토의 0.1M HCl 침출 Cu 함량은 0.36 mg kg^(1) 및 0.28 mg kg^(1)이었으며, 경작년수가 오래될수록 함량은 계속 증가하여 30년 이상 된 감귤원에서는 비경작지에 비해 96배 및 52배 증가하였다. 연평균 증가량은 표토가 약 1 mg kg^(1), 심토가 약 0.5 mg kg^(1)이었다. 또한, 비경작지에서 0.1M HCl 침출 Zn 함량은 각각 0.37 mg kg^(1) 및 2.42 mg kg^(1)로서 Cu에 비해 높았으며, 경작년수가 오래될수록 함량은 증가하였으나 증가량은 Cu에 비해 낮았다. 연속침출법으로 측정한 Cu와 Zn의 수용성과 KNO_(3) 침출형태는 검출되지 않았으며, 주요 분포 형태는 EDTA 침출형태로서 토양 중 Cu와 Zn이 토양과 강하게 결합된 형태로 존재하였다. 각 형태별 함량의 합에 대한 EDTA 침출성 Cu와 Zn의 비율은 경작넌수가 오래됨에 따라 커졌으며, Na_(4)P_(2)O_(7) 침출성 Cu와 HNO_(3) 침출성 Zn 형태는 반대로 감소하였다. 경작년수에 따라 증가량이 가장 큰 형태는 EDTA 침출형태였으며, 상대적으로 증가량이 적은 형태는 Na_(4)P_(2)O_(7)이었다. 0.1M HCl 침출성 Cu와 Zn 및 각 형태별 함량의 심토/표토비는 경작년수가 길어짐에 따라 낮아져서 토양으로 유입된 Cu와 Zn이 주로 포토에 집 적되고 있는 것으로 나타났다. This study was conducted to determine changes in Cu and Zn content in citrus orchard soils amended with compost, pesticide, and chemical fertilizers enriched Cu and Zn. Soils were collected from citrus orchards in Namcheju area distributed volcanic ash soils. The soil samples were non-cultivated, cultivated for 5 years, 10-15 years, and more than 30 years, respectively. Cu and Zn were extracted by 0.1M HCl and by sequential fractionation method. 0.1M HCl extractable Cu content in non-cultivated surface and subsoil were 0.36 and 0.28 mg kg^(-1), respectively and their content increased with years of citrus cultivation. Cu content in surface and subsoil cultivated for more than 30 years increased 96 and 52 times compared to non-cultivated soils with annual increase of about 1.0 and 0.5 mg kg^(-1). 0.1M HCl extractable Zn increased same as Cu, however, annual increase was less than Cu. Water soluble and KNO_(3) extractable Cu and Zn by sequential extraction were not detected. The predominant fraction of Cu and Zn was found to be EDTA extractable fractions form, which is strongly adsorbed to soil. EDTA extractable Cu and Zn fractions to sum of fractions increased with years of cultivation while Na_(4)P_(2)O_(7), extractable Cu and HNO_(3) extractable fractions Zn decreased. Subsoil/surface ratio of 0.1M HCl extractable Cu and Zn and the fractions were decreased with years of cultivation.

Bacillus subtilis BS 62의 γ-glutamyltranspeptidase 유전자 해석

이태은 忠南大學校 大學院 2003 국내석사

리간드 화합물로써 2,9-(N,N-Dimethylethylenediamino)- 1,10-Phenanthroline의 합성과 X-ray 결정분석

朴京用 忠南大學校 大學院 2005 국내석사

The novel macrocyclic compounds (Ⅰ, Ⅱ) and 2,9-(N,N-Dimethylethylenediamino)-1,10-phenanthroline were synthesized and these structures were remarkably identified by ¹H-NMR, ^(13)C-NMR and X-ray analyses. The results base on these experiments are summarized as follow; 1. The 2,9-(N,N-Dimethylethylenediamino)-1,10- phenanthroline as ligand was synthesized through 4 steps from 1,10-phenanthroline as a starting compound. As a result of ¹H-NMR analysis, it was disclosed the 12 protons of -CH₃group presenting at δ_(H) 2.32 ppm and the 4 protons of -CH₂- group presenting with triplet state at δ_(H) 2.65~3.69 ppm, respectively. Also, the 6 protons of 1,10-phenanthroline were disclosed at δ_(H) 6.72~7.86 ppm. It was revealed that the presence of proton range in δ_(H) 7.83~7.86 ppm and 6.74~6.77 ppm was showed coupling constant values wih J=8.7 Hz. In a result of ^(13)C-NMR analysis, it was showed the carbon peak of -CH₃ group at δ_(C) 39.18 ppm, -CH₂- group at δ_(C) 45.21~58.30 ppm, and the aromatic carbon peak of 1,10- phenanthroline at δ_(C) 110.70~157.03 ppm. In this result investigated of carbon peaks, the carbon number was fined as 20. 2. The result of X-ray analysis, the 2,9-(N,N-Dimethylethylenediamino)-1,10- phenanthroline was included two moleculars with water as a monoclinic compound. It was formed the plane structures that had the torsion angle values with 6.2 and 179.5 on the C(4)-N(2)-C(5)-N(3) and C(17)-N(5)-C(16)- N(4). A torsion angle between the oxygen of a phosphate diester coordinated with metal and N2, N5 atoms expected to bind with hydrogen was showed value with 79.4°(as a N(1)-C(3)-C(4)-N(2)). And, a torsion angle between the oxygen of leaving group and N1, N6 atoms expected to bind with hydrogen was showed value with 60.8°(as a N(5)-C(7)-C(18)-N(6)). The difference of these torsion angles was 18.6° 3. As a result of ¹H-NMR analysis of macrocyclic compound(I), it was disclosed the 8 protons of -CH₂- group presenting at δ_(H) 4.10 ppm and the 16 protons of 3,6-Bis(pyridyl)pyridazine presenting at δ_(H) 7.39~9.00 ppm, respectively. And it was revealed that the presence of proton range in δ_(H) 8.70~8.73 ppm and 7.36~7.39 ppm was showed coupling constant values wih J=7.8 Hz. In a result of ^(13)C-NMR analysis, it was showed the carbon peak of -CH₂- group at δ_(C) 63.98 ppm and the aromatic carbon peak of 3,6-Bis(pyridyl)- pyridazine at δ_(C) 120.71~158.87 ppm. In this result investigated of carbon peaks, the carbon number was fined as 32. 4. As a result of ¹H-NMR analysis of macrocyclic compound(II), it was disclosed the 4 protons of -NH- group presenting at δ_(H) 3.86 ppm and the 8 protons of -CH₂- group presenting at δ_(H) 4.72 ppm, respectively. Also, the 20 protons of benzene and 1,10-phenanthroline were disclosed at with doublet, multiplet state δ_(H) 6.84~8.14 ppm. It was revealed that the presence of proton range in δ_(H) 8.10~8.14 ppm and 7.92~7.96 ppm was showed coupling constant values wih J=9.0 Hz. In a result of ^(13)C-NMR analysis, it was showed the carbon peak of -CH₂- group at δ_(C) 48.69 ppm and the aromatic carbon peak at δ_(C) 123.85~143.27 ppm. In this result investigated of carbon peaks, the carbon number was fined as 40. 본 연구에서는 인공 핵산분해 효소로서의 기능이 예상되는 전이 금속 착화합물을 합성하기 위한 전단계로써, 리간드 화합물인 2,9-(N,N-dimethyl- ethylenediamino)-1,10-phenanthroline 을 합성하고, X-ray 회절 분석을 통해 삼차원적 구조를 확인하였다.

C1과 SO4처리가 감귤의 무기물과 당 및 유기산의 조성에 미치는 영향

송성준 제주대학교 대학원 1985 국내박사

대두 배양세포의 생장과 단백질에 대한 Cytokinin의 경향

김창오 제주대학교 대학원 1988 국내박사

젖산발효에 미치는 율무쌀 첨가 효과에 관한 연구

진용서 圓光大學校 1992 국내석사