Study of van der waals interactions in graphene

Ahn, Sungjoon Sungkyunkwan university 2017 국내박사

Thermophysical property and material compatibility of accident-tolerant UO2-U3Si2 composite fuel

Jungsu Ahn Ulsan National Institute of Science and Technology 2023 국내박사

One of the important lessons learned from the Fukushima accident was the low thermal conductivity of UO2 is not something supposed to be overlooked, at least not any longer. The most widely used commercial nuclear fuel, UO2, in fact has the lowest thermal conductivity among all major types of nuclear fuel including U-Zr, U-Mo, UN, UC, and U3Si2. After the accident, many accident-tolerant fuel (ATF) concepts, usually with enhanced thermal conductivity, have been resuscitated and newly suggested; however, after over a decade of research, most of them are still in the testing phase. Uranium sesquisilicide (U3Si2) is a strong candidate material for ATF since it can simultaneously provide 4-8 times higher thermal conductivity and ~16% higher fissile density than UO2 unlike other non-fissile higher thermal conductivity additives considered for nuclear fuel. Similar benefits could be expected by adopting uranium mononitride (UN) and uranium monocarbide (UC); however, their corrosion resistance in for water-cooled reactor is generally considered unacceptable. The corrosion resistance under water/steam environment is also an outstanding issue for U3Si2, but perhaps considerably manageable. For instance, shorter fuel rod design with reduced fuel pellet diameter has been suggested mainly by Westinghouse Electric Company (WEC) to mitigate the consequence of cladding rupture; however, it will certainly increase the fuel cost. The in-reactor corrosion issue of U3Si2 could be more economically handled by adopting UO2-xU3Si2 (x < ~50 wt%) composite fuel. In this approach, the concerns are laid on the potential microstructural defect formation between UO2 and U3Si2, e.g., micro-crack and secondary phase formation, which may deteriorate the fuel thermal conductivity and the corrosion resistance, which are the main subject of this dissertation. A comprehensive experimental investigation on UO2-xU3Si2 (x = 10, 30, and 50 wt%) composite has been conducted in particular order to examine its applicability as an accident-tolerant fuel for water-cooled reactors. Firstly, the fuel manufacturing process was developed, for both conventional sintering (CS) and spark plasma sintering (SPS), using a surrogate material (CeO2-xCeSi2) to minimize radioactive waste generation during the development phase. Then, the UO2-U3Si2 pellets using UO2.02 and UO2.15 powder have been prepared and characterized using X-ray diffractometry (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDS), and laser flash analysis (LFA). The stoichiometric UO2.02-U3Si2 was selected as as-fabricated or beginning-of-cycle (BOC) composite fuel since the interaction layer (IL) formation between UO2 and U3Si2 has to be suppressed during the fabrication. In contrast, hyper-stoichiometric UO2.15-U3Si2 was considered as an end-of-cycle (EOC) composite fuel assuming a significant discharge burnup that might be challenged for advanced reactor designs. Finally, with the fully characterized fuel pellets, the oxidation resistance of UO2-U3Si2 was evaluated under high temperature air and pressurized water environment using thermogravimetric analysis (TGA). Diffusion couple study of UO2-U3Si2 with a selected ATF cladding material, FeCrAl alloy, was also carried out to glimpse the material compatibility of the composite fuel. From the surrogate tests (CeO2 instead of UO2, and Ce3Si2 for U3Si2), spark plasma sintering was selected over conventional sintering based on simultaneously obtained higher pellet density and homogeneous microstructure; thus, all UO2-U3Si2 composite pellets used for this study were spark plasma sintered. In the SPS-ed UO2-xU3Si2 (x = 10, 30, 50 wt%) pellets, the formation of U3Si inclusions and USi phase regions was confirmed, which was more significant in high temperature (> 1400 °C) SPS-ed pellets. With increasing U3Si2 composition and sintering temperature, the SPS-ed pellet density per theoretical density was monotonically increased up to ~93%TD. The thermal conductivity of UO2-U3Si2 composite was decreased with increasing temperature, while the amount of decrement was decreased with increasing U3Si2 composition. Hyper-stoichiometric UO2.15-50wt% U3Si2 pellets exhibited 5-7% lower thermal conductivity than that of UO2.02 due to significant secondary USi phase zone formation. In contrast, stoichiometric UO2-50 wt%U3Si2 exhibited up to 100% higher thermal conductivity than UO2, which may suggest progressively accelerated formation of interaction layer and secondary phase with increasing burnup. Significantly enhanced oxidation and corrosion resistance of UO2-U3Si2, compared to U3Si2, was concluded from the following results: (1) ~130 ℃ higher onset temperature in dynamic oxidation in air, (2) ~50% less weight gain for isothermal oxidation at 300 ℃ in air, and (3) noticeably mitigated pellet degradation under high temperature pressurized water. The diffusion couple study on UO2-U3Si2 composite with an FeCrAl alloy showed the formation of 5-10 μm thick U-Si-O layer after 100 h annealing at 600 ℃. With further elevated annealing temperature of 800 ℃, U and Si infiltration to FeCrAl matrix was also observed after 100 h annealing, which may potentially limit the reactor operating temperature or the use of the FeCrAl alloy with the composite fuel. This series of metallurgical and thermophysical investigations partially demonstrated the feasibility of UO2-U3Si2 composite as an accident-tolerant fuel, which might be an only option to offer both higher thermal conductivity and higher fissile density at the same time. It also indicated that suppressing the formation of secondary phases (U3Si and USi) with lower melting temperature and/or thermal conductivity during the fuel fabrication and reactor operation is a key to the successful utilization of the type of inhomogeneous composite fuel. Thus, concerning irradiation-accelerated interaction layer formation between UO2 and U3Si2, further experimental demonstration of the safety and performance of the composite fuel needs to be carried out as a future study, which could utilize, if not in-pile test in a research reactor, in-situ heating ion-beam irradiation under various mechanical stress and temperature conditions matching to an advanced reactor design under consideration.

Diagnosis of Canine Chronic Mitral Valve Insufficiency by Evaluating Growth Differentiation Factor 11 and Interventricular Septal Wall Excursion

Ahn, Sung Taek Kangwon National University 2018 국내박사

국문초록 GDF 11과 ISWE의 평가를 통한 개의 CMVI에 대한 진단 안 성 택 강원대학교 대학원 수의학과 CMVI(Chronic mitral valve insufficiency)는 일반적으로 개, 특히 소형견에서 가장 흔한 심장병의 하나이며, 만성적인 이첨판의 점액변성으로 이첨판 폐쇄부전을 유발하여 울혈성 심부전(congestive heart failure, CHF)을 초래한다. GDF 11(Growth differentiation factor 11)은 BMP(bone morphogenetic protein) 계열의 일종이며, 배아 및 성인 조직 모두에서 세포 성장과 분화를 조절하는 인자이다. 또한, GDF 11이 SERCA-2 의 발현 증가를 통해 심근 확장 기능을 향상시키는 연구보고가 있으며, 마우스 연구에 있어 GDF 11 혈청농도는 나이들수록 낮아지고 심장질환을 가진 개체에서도 낮은 농도를 나타냈다. CMVI도 노화와 연관된 심장질환으로 GDF 11의 농도가 낮을 수 있다는 것을 유추할 수 있다. 본 연구의 가설은 CMVI를 가진 개에서 혈청 GDF 11농도가 낮아지며, 심장병의 진행이 더 빠르게 진행될 수 있다는 것이다. 이는 GDF 11의 혈청 농도는 CMVI를 가진 개를 진단할 수 있는 좋은 지표가 될 수 있다는 근거가 된다. 본 실험을 위해 11마리의 건강한 개와 CMVI가 있는 69마리의 개가 선발되었으며 연령,체중등의 신체검사 및 혈액검사,심장초음파검사등의 임상검사를 통해서 CMVI그룹은 ISACHC기준에 따라 심부전을 분류했다. 연령과 심부전에 따른 혈청 GDF 11농도를 평가하고 분석한 결과,혈청 GDF 11농도는 심장질환을 나타내는 여러 indices 및 연령과 상관관계가 없게 나타났다. 따라서 마우스실험에서 확인된 심장병이 있는 나이든 마우스의 혈청 GDF 11농도가 감소한다는 이론은 CMVI를 가진 개의 실험에서는 확인되지 않았다. 좌심실과 우심실사이에 있는 중격벽의 움직임인 ISWE는 이전의 사람 및 동물 실험을 통에서 좌심실의 부하가 증가할 때나 심박수가 급격하게 증가할 때 과잉움직을 보이는 것을 알아냈다.이러한 과잉움직임은 만성심장병을 가진 개에 있어 대상부전을 의미하기도한다.개의 CMVI는 만성적으로 좌심실의 부하가 증가하는 심장병이다.이는 CMVI를 가진 개에 있어 ISWE가 증가하며 심부전이 진행될수록 증가할 수 있다는 것을 유추할 수 있다.본 실험은 CMVI를 가진 개에 있어 ISWE가 증가하며 심부전이 심해질수록 증가한다는 가설을 세워 진행했다.이는 ISWE가 CMVI의 진단을 위한 좋은 지표가 될 수 있다는 근거가 된다.본 실험을 위해 18마리의 건강한 개와 CMVI를 가진 51마리의 개가 선발되었으며 연령.체중등의 신체검사 및 임상검사를 통해서 CMVI그룹은 ISACHC기준에 따라 심부전을 분류했다.심장초음파 검사를 통해서 ISWE를 평가하고 분석한 결과 심장질환을 나타내는 심장초음파지표 및 바이오마커와 상관성이 높은 것으로 밝혀졌다.따라서 ISWE는 CMVI진단 및 중증도를 평가하는 데 있어 우수한 지표가 될 수 있다. □ 핵심주제어 바이오마커, 성장분화인자 11(GDF 11), 개, 만성이첨판폐쇄부전, 심실간 중격 벽 유출 Diagnosis of Canine Chronic Mitral Valve Insufficiency by Evaluating Growth Differentiation Factor 11 and Interventricular Septal Wall Excursion Sung-Taek Ahn Department of Veterinary Medicine, Graduate School, Kangwon National University ABSTRACT In general, chronic mitral valve insufficiency (CMVI) as a cardiac disease is commonly found in dogs, in particular, small breed. CMVI takes place because of chronic myxomatous mitral valvular degeneration. It triggers mitral regurgitation, which consequently leads to the congestive heart failure (CHF). Growth differentiation factor 11 (GDF 11) is one of bone morphogenetic proteins (BMP). It is a regulator of cell growth and differentiation in both embryonic and adult tissues. In addition, other studies report that GDF 11 improved myocardial diastolic function through the increase in the manifestation of SERCA-2. In case of a study using mice, it was indicated that the serum level of GDF 11 became lower as the age of mice was higher. In this sense, since CMVI as a cardiac disease is related to aging, it infers that the serum level of GDF 11 might be lower. This study hypothesizes that dogs with CMVI would have the low serum level of GDF 11 and the progression of heart disease might be more quickly accelerated. Therefore, it is the evidence that the serum level of GDF can be a good marker to diagnose dogs with CMVI. 11 healthy dogs and 69 dogs with CMVI were examined for this study. All selected dogs' age and body weight were investigated. Physical examination, blood test and echocardiography for CMVI dogs were conducted. The degree of cardiac insufficiency in CMVI dogs was sorted out by the criteria of ISACHC. The result from evaluating and analyzing the serum level of GDF 11 depending on age and heart failure showed that there was no correlation between the serum level of GDF 11, age, and various indices representing heart disease. Thus, the hypothesis that the serum level of GDF in an old mouse with heart disease decreases was not identified in dogs with CMVI. Previous studies with clinical tests and animal experiments found that interventricular septal wall excursion (ISWE) located in the gap between the left ventricle and the right ventricle excessively moved when the load of the left ventricle increased or heart rate dramatically increased. These excess movement of ISWE in dogs with CMVI represents a decompensatory response. Dogs' CMVI refers to heart disease that the load of the left ventricle chronically increases. It infers that dogs with CMVI have an increase in ISWE, and ISWE can rise as cardiac insufficiency deteriorates. The experiment for this study was conducted with the hypothesis that ISWE in CMVI dogs increases and the increment in ISWE takes place when cardiac insufficiency deteriorates. It provides the evidence that ISWE could be a good index to diagnose CMVI dogs. 18 healthy dogs and 51 dogs with CMVI were investigated for this study. All dogs' age and body weight in this study were examined. Physical examination and a medical test were conducted. The degree of cardiac insufficiency in CMVI dogs was classified by the criteria of ISACHC. The result from evaluating and analyzing ISWE by echocardiography indicated that ISWE was closely correlated with echocardiographic indices representing heart disease and a cardiac biomarker. Accordingly, ISWE can be a good indicator to diagnose CMVI dogs and evaluate the severity level of CMVI. □ Key words biomarker, growth differentiation factor 11 (GDF 11), dog, chronic mitral valve insufficiency (CMVI), interventricular septal wall excursion (ISWE)

Forms and functions of English tag questions : a corpus-based study

안소영 Chonnam National University 2018 국내석사

Korean learners have shown weaknesses in practical communication applications, while they have excellent grammatical and lexical skills. This is because they show much strength in mechanical learning on syntactic and semantical aspects of tag questions but have been experiencing a lot of difficulties in applying tag questions in actual use. Therefore, it is imperative to analyze the various forms and functions used in authentic situation. In addition to the strategic role and function of tag questions to facilitate communication, a study on practical use that has been somewhat neglected in the education field. Although the study of typical tag questions in the use of English texts have been relatively active, there have been few studies on exceptionally diverse uses. Moreover, in Korean schools, tag questions which have the same polarity or inconsistent verb with the main clause are accepted as the wrong grammatical form. This was found to be significantly different from actual use. Therefore, this study analyzes the use of various forms of tags through the corpus analysis study and examines the functions according to them. To do this, Three research tasks have been presented and studied: Firstly, what the most frequently used English tag questions; secondly, How tag questions appears in various forms in terms of polarity, subject, auxiliary value; and lastly, what functions tag questions exhibit in utterance situations. As a result of the analysis, it was confirmed that the tag questions on the corpus was not consistent with the verb or subject of the sentence, unlike the normative grammar. Although the grammatical elements are specified, the actual use of tag questions does not fit into this framework and it shows various forms depending on various practical use. Especially in case of polarity, it was observed that the tags in the same polarity shows high proportion of all tags. It was also found that tag questions are used in various forms in terms of subject and auxiliary value through data. In addition, it was indicated that there exist a wide range of functions of tag questions. Consequently, it was advised that pedagogical effort should be made to make students apply tag questions into practical use. Through the authentic practice of tag questions, it is expected that the overall english speaking ability will be improved as well.

Antiangiogenic and antitumor constituents from vietnamese medicinal plants and their analogues : Nam, Nguyen-hai

Nam, Nguyen-hai 충남대학교 대학원 2002 국내박사

Dispersive Finite-difference Time-domain Analysis of Invisibility Cloaks

Lee,Yongyoon The Graduate School of the University of Seoul 2013 국내박사

Visible light-driven photocatalytic control of emerging pharmaceutical micropollutants from water and wastewater using microbubble Upflow airlift reactor (UALR) system

Fahmida, Ishaque 영남대학교 대학원 2024 국내박사

Confirmation of Quantitative Trait Loci (QTL) for spikelets per panicle and awn length using near isogenic lines derived from an interspecific cross between Oryza sativa and O. minuta

LUONG NGOC HA 충남대학교 대학원 2018 국내석사

Rice (Oryza sativa L.) grain yield is determined by four yield components: panicles per plant, spikelets per panicle, grain weight and spikelet fertility. Among them, spikelets per panicle (SPP) shows a variation and makes the large contribution to yield output and was the major objective of high-yield breeding. In our previous study, one putative QTL affecting the number of spikelets per panicle (qSPP7) was identified on the long arm of rice chromosome 7 across a 28.6-kb region using near-isogenic lines (NILs) derived from an interspecific cross between the Korean japonica ‘Hwaseongbyeo’ and O. minuta. In the current study, we confirmed this QTL using 540 BC8F2 plants for genotyping and 13 recombinant BC8F3 lines for trait evaluation. The qSPP7 was mapped between two simple sequence repeat (SRR) markers RM4952 and RM21605. The O. minuta segment on chromosome 7 introgressed into the ‘Hwaseongbyeo’ background was associated with an increase in the number of spikelets per panicle. Additional experiment under short-day length condition indicated that NIL-SPP7 was photoperiod sensitive and qSPP7 was not associated with heading date. Like many other important traits in plant breeding, awn length is also a complex trait controlled by multiple genes. In our previous study, the qawn9 QTL was identified on chromosome 9 in NILs derived from an interspecific cross between Hwaseongbyeo and O. minuta. To continue our previous research, this study was carried out to confirm and narrow down the target region containing the QTL associated with awn length on chromosome 9 using a F2 population derived from a cross between the Korean indica cultivar ‘Milyang 23’ and NIL-Awn9 (derived from a cross between ‘Hwaseongbyeo’ and O. minuta). QTL analysis was carried out to determine the relationship between marker genotype and awn length. We confirmed that O. minuta allele in the target QTL awn9 locus within 96-kb interval between markers VNR10 and RM215 controlled awn development. Thus, the identified QTL and their linked-markers could become a valuable source for the map-based cloning of the gene controlling awn development. 벼의 수량성은분얼수,수당립수,종자중,종자의 임실률에 의해 결정된다.이 중수당립수는 다양한 변이를 보이고수량 증가에 크게 기여하며, 다수성을 목표로 한 육종에서 매우 중요한 요인이다.이전의 연구에서 한국 자포니카 품종인 화성과 야생벼인O. minuta교배 후대 근동질 계통을 이용하여수당립수를 증가시키는 양적 유전자좌qSPP7(spikelet per panicle)이 7번 염색체 28.6-kb 지역에 위치하는 것을 확인하였다.본 연구에서는 540개의 BC8F2개체의 유전자형을 검정하고 그 중 13개의 BC8F3재조환 계통의 표현형을 조사하여 수당립수를 조절하는 QTL위치를 재확인하였다.qSPP7은 SSR마커인 RM4952와 RM21605 사이에 위치하는 것을 알 수 있었다.화성벼의 유전적 배경에 이입된 O. minuta염색체 단편이 수당립수 증가에 관련있는 것으로 보인다.추가적인 실험에서 단일조건에 NIL-SPP7은 일장감응에 민감한 것으로 나타났으며,이는 qSPP7이 출수기와 연관되지 않음을 의미한다. 식물 육종에 있어 중요한 다른 형질들과 같이 까락길이도 다수의 유전자에 의해 조절된다.선행 연구 결과에서 qawn9는 화성벼와O. minuta종간교잡 후대 근동질 계통에서 탐지되었고,9번 염색체에 위치하는 것을 확인하였다.본 논문에서는 한국 인디카 품종인 밀양23호와 NIL-Awn9 (화성벼와O.minuta교배 후대)를 교배하여 얻은 F2계통을 이용하여 양적유전자좌 범위를 좁히고, 분자 표지를 통해 확보한 유전자형과 표현형과의 관계를 확인하였다. 그 결과 awn9은 분자 표지인 VNR10과 RM215사이에서 확인되었으며,그 거리는 96-kb인 것으로 나타났다.이러한 연구 결과로 통해 밝혀진 양적유전자좌와 연관된 분자 표지들은 까락의 발달을 조절하는 유전자를 밝히는데 유용하게 활용될 것으로 사료된다.

Study on improved electroactivity of transition metal based materials for Energy Storage System

Jae-Young, Kim 영남대학교 대학원 2024 국내석사

슈퍼커패시터는 산업이 발전함에 따라 고용량, 고출력의 연구가 선행되었다. 배터리-타입 슈퍼커패시터는 충/방전 메커니즘에 의해서 고용량 고출력의 특성을 지니며 차세대 에너지 저장 장치로 각광받고 있다. 그 중 코어쉘 구조의 전극 물질은 풍부한 표면 활성 면적과 이온 이동거리의 감소와 같은 시너지효과로 인해 이상적인 구조로 여겨진다. 이 논문에서는 우레아와 플루오린화 암모늄 첨가제를 이용한 수열합성을 통해 코발트 계열의 중간물질인 Co2(CO3)(OH)2를 수직으로 합성하고 셀렌화를 통해 코어 물질인 CoSe 합성 후 정전압 전기증착을 통하여 코어쉘 구조의 CoSe@CoNi-LDH를 바인더 없이 합성하였다. 코어쉘 구조의 특징으로 인해 CoSe@CoNi-LDH 전극 물질은 높은 비정전용량 (1015 C g⁻¹ at 1 A g⁻¹)을 나타내고 우수한 속도 특성 (65% at 20 A g⁻¹)을 나타낸다. 또한 10000사이클 후에도 초기용량의 84.38%를 유지하는 놀라운 사이클 안정성을 보여준다. 이 논문은 슈퍼커패시터의 기본 작동원리를 소개하고 전기화학적 에너지 저장 장치를 위한 코어쉘 구조의 통찰력을 제공한다. In this study, we present the successful synthesis of a novel core-shell structure, featuring cobalt selenide nanowires adorned with nickel cobalt layered double hydroxide (CoSe@NiCo-LDH). The architecture comprises CoSe nanowires encapsulated within NiCo-LDH nanosheets on a nickel foam (NF) substrate, achieved through a facile three-step synthesis method. Initially, core cobalt carbonate hydroxide hydrate nanowires (CCHH) were grown on an NF substrate using a straightforward hydrothermal approach. Subsequently, CoSe nanowires were obtained through a selenization process, and finally, a shell of NiCo-LDH nanosheets was deposited via an electrodeposition method. Leveraging the intrinsic advantages of this core-shell architecture and its unique composition, the resulting CoSe@NiCo-LDH material exhibited an impressive specific capacity of 1015 C g⁻¹ at 1.0 A g⁻¹, demonstrating exceptional rate capability with a performance retention of 65.0% even at a high current density of 20 A g⁻¹. Moreover, it displayed remarkable cyclic stability, retaining 84.38% of its initial capacity after 10,000 cycles. These findings underscore the potential of core-shell architectures for developing high-performance supercapacitors with improved kinetics and stability.

Mesenchymal stem cell-derived secretome attenuates nephritis in NZB/W F1 mice by modulating the inflammatory response

안성수 Graduate School, Yonsei University 2020 국내박사

Mesenchymal stem cell-derived secretome attenuates nephritis in NZB/W F1 mice by modulating the inflammatory response Sung Soo Ahn Department of Medicine Yonsei University Graduate School of Medicine (Directed by Professor Yong-Beom Park) Rationale & Objective: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by alterations of the immune system, resulting in multiple organ injury. Accumulating evidences suggest mesenchymal stem cells (MSC)-derived secretome exert anti-inflammatory properties; however, the therapeutic efficacy of secretome in SLE is not well understood. This study aimed to investigate the efficacy of secretome on murine and human lupus. Method: Fifty-two NZB/W F1 mice were randomly assigned into 4 groups (untreated, methylprednisolone, MSCs, and secretome). The amount of proteinuria, histological damages were evaluated, and renal deposition of immune-complexes was assessed by immunofluorescence staining. Serum cytokines, anti-dsDNA, and IgG2a subclass were also determined. Helper T (Th) cell, plasma cell, dendritic cell, and macrophage markers were analyzed using a fluorescence-activated cell sorter. CD4+ T cells isolated from the blood of SLE patients were used to analyze the effect of secretome in human SLE, and the secreted proteins associated with immunoregulation was assessed by proteomics. Results: Mice treated with methylprednisolone, MSC, and secretome exhibited diminished proteinuria, improved renal injury, and reduced renal deposition of immune-complexes compared to untreated mice. Regarding Th cell subsets, the proportion of Th1 and Th2 cells was decreased and regulatory T cells (Tregs) were increased in secretome treated mice. Secretome treatment also decreased the expression of mature dendritic cells and M1 macrophage markers and the number of plasma cells. Serum cytokine levels of interferon-γ, interleukin (IL)-17A, anti-dsDNA, and IgG2a subclass decreased upon secretome treatment, whereas IL-10 level was increased. Administration of secretome did not result in an increased toxicity in mice, and the proportion of Tregs increased when CD4+ T cells from SLE patients were treated with secretome. Twenty-one proteins were identified as putative candidates of immunomodulation in secretome. Conclusion: The results from this study suggest that secretome could be a promising therapeutic agent for the treatment of SLE. NZB/W F1 마우스에서 중간엽 줄기세포 유래 단백질을 이용한 염증 반응의 감소와 신염 조절 < 지도교수 박 용 범 > 연세대학교 대학원 의학과 안 성 수 배경: 전신 홍반성 루푸스는 면역 체계의 변화에 의하여 다발성 장기 손상이 발생하는 자가면역 질환이다. 최근의 연구들은 중간엽 줄기세포 유래 단백질이 항염증 작용을 하는 것으로 보고하고 있으나 전신 홍반성 루푸스에서의 효과에 대해서는 잘 알려져 있지 않다. 목적: 본 연구에서는 전신 홍반성 루푸스의 동물 모델과 사람에게서 중간엽 줄기세포 유래 단백질의 효능을 확인하고자 하였다. 방법: 52마리의 NZB/W F1 마우스를 총 4개의 그룹 (비치료군, 메칠프레드니솔론군, 중간엽 줄기세포군, 그리고 중간엽 줄기세포 유래 단백질군) 으로 나누어 실험을 진행하였다. 마우스에서 단백뇨의 양, 조직학적인 손상의 정도를 평가하였으며 신장내의 면역복합체의 침착은 면역형광 염색으로 평가하였다. 혈청내 사이토카인, 항-dsDNA, 그리고 IgG2a 의 아형을 같이 분석하였다. 보조 T 세포, 형질세포, 수지상 세포, 대식세포의 표지자들을 형광표지세포분류기로 확인하였다. 전신 홍반성 루푸스 환자의 혈액에서 CD4+ T 세포를 분리하여 이에 미치는 중간엽 줄기 세포 유래 단백질의 효과를 분석하였으며 중간엽 줄기세포 유래 단백질 내에 면역 조절과 연관된 단백질들은 프로테오믹스를 이용하여 확인하였다. 결과: 메칠프레드니솔론, 중간엽 줄기세포, 그리고 중간엽 줄기세포 유래 단백질을 투약한 마우스는 단백뇨, 신손상, 그리고 신장내 면역복합체의 침착이 비치료군에 비해서 유의하게 감소하였다. 중간엽 줄기세포 유래 단백질을 투여한 마우스에서 보조 T 세포의 아형 중 Th1 과 Th2 세포가 감소하였으며 조절 T 세포는 증가하였다. 또한, 성숙한 수지상 세포와 M1 대식세포의 표지자는 감소하였으며 형질 세포의 숫자 또한 감소하였다. 중간엽 줄기세포 유래 단백질을 투여한 경우에 혈청내 인터페론 감마, 인터루킨-17A, 항-dsDNA, 그리고 IgG2a의 아형이 감소하는 것으로 확인되었으며 IL-10의 경우는 증가하였다. 중간엽 줄기세포 유래 단백질을 동물에게 투약하였을 때 유의한 독성은 확인되지 않았으며 전신 홍반성 루푸스 환자의 CD4+ T 세포에 중간엽 줄기세포 유래 단백질을 처리하였을 때에도 조절 T 세포의 숫자가 증가함이 확인되었다. 프로테오믹스를 통해서 중간엽 줄기세포 유래 단백질 내에서 총 21개의 단백질이 면역 조절과 연관될 수 있음을 확인할 수 있었다. 결론: 본 연구의 결과를 통해서 중간엽 줄기세포 유래 단백질은 루푸스에서 유망한 치료제가 될 수 있을 것으로 생각된다.