추천시스템에서 엔트로피와 Default voting을 이용한 사용자 유사도 측정 방법

조선호 仁荷大學校 大學院 2002 국내석사

재실자의 환경적응행동과 건물에너지 소비량

조선호 경희대학교 2014 국내석사

The building sector accounts for about 25% of the global energy consumption in Korea. Especially, approximately 53% of the energy used in the building has been consumed for heating, ventilating, and air-conditioning(HVAC). In the case of commercial and public buildings, every year that cooling energy consumption gets higher and higher. Historically, buildings are one of the means for human to adapt to the environment, and humans created comfortable environment that by adapted for thermal environment and taking action. In addition, cooling and heating operating time is increasing because of the increasing occupancy time and global warming, which is directly linked with building energy consumption. Thus, the understanding for indoor comfort condition are indispensible for building energy analysis because of the energy used in HVAC system operation play an important role in providing thermal comfort. It is also significant to ensure the thermal comfort along with the reduction of building energy consumption. In indoor environment, not only the reduction of building energy but also the thermal comfort is an important factor for the health of building occupants. For evaluating thermal comfort, precise thermal comfort model is necessary. According to the previous studies, PMV model has an error that is different between PMV and the occupant’s actual thermal sensations. That could become key drivers for the profligate consumption of energy. On the other hand, Adaptive comfort theory that thermal comfort zone is increased in the process of adaptive action and thermal adaptation is mainly based on field study on the occupant's actual living conditions. Thus, understand the relationship between adaptive behaviour, thermal comfort and building energy that will help to prediction of energy demand and the energy efficiency of HVAC system. This study aims to reduce the energy consumption of a building and improvement of the thermal comfort of building occupants by develop the Adaptive Comfort Model considering adaptive behaviours of building occupants. A field measurement and questionnaire survey was conducted to analyze the impacts of adaptive behaviours on thermal comfort, and then develop and verify the Adaptive Comfort Model. A field measurement was conducted for eight room in seven types of buildings to analyze the adaptive behaviours by occupants, and constructs a indoor thermal condition, thermal comfort, and adaptive behaviours database. Moreover, the experiment was carried out to investigate physical factor, behavioural adaptation, psychological adaptation and comprehensive factor. Adaptive Comfort Model was developed and verified using existing field measurement data. Finally, existing and new measured field data was combined to develop the modified Adaptive Comfort Model to improvement in the prediction performance and to reflect a variety of building characteristics. The building simulation tool, EnergyPlus, was used to estimate the building energy consumption. Adaptive Comfort Model was compared with existing model(ASHRAE) to evaluate the building energy consumption. The results showed that comfort temperature differ depending on multiple relationship on environmental factors. There is a strong correlation between neutral temperature and actual thermal sensation of occupants. Also, the behavioural adaptation and psychological adaptation influence on thermal sensation and thermal comfort. The Adaptive Comfort Model was developed from rigorous statistical analysis of exponentially weight mean outdoor temperature and neutral temperature. Adaptive Comfort Model's comfort range was fixed for ±2K. Prediction performance results showd that the proportion that is included in the comfort temperature range, 61.6% from KyungHee university, and 50.0% from S office building. So it is required to implement a variety of building characteristics in order to improvement prediction performance. Existing and new measured field data was combined to develop the modified Adaptive Comfort Model. It was obtained from regression analysis using exponentially weight mean outdoor temperature and neutral temperature and separated by heating and cooling models. (Cooling model: Tc=0.210*Trm+20.833, Heating model: Tc=0.053*Trm+24.151) Simulation results indicated the cooling energy consumption with 'comfort mode' was higher, followed by 'ASHRAE condition', 'standard mode', 'energy-saving mode', and heating energy consumption with 'comfort mode' was higher, followed by standard mode', 'energy-saving mode', 'ASHRAE condition'. When 'energy-saving mode' and 'standard mode' of Adaptive Comfort Model were applied, building energy consumption could be reduced compare to the 'ASHRAE condition' in cooling period. In addition, considering that annual building energy consumption, 'energy-saving mode' of Adaptive Comfort Model was most energy efficiency. 우리나라의 최종에너지 소비에서 건물부문이 차지하는 에너지 소비는 약 25% 수준이다. 특히, 냉ㆍ난방에너지는 건물에서 사용되는 에너지의 약 53%를 차지하고 있으며, 상업 및 공공용 건물의 경우 냉방 에너지소비가 매년 크게 증가는 추세이다. 역사적으로 건축물은 인간이 환경에 적응하는 수단의 하나였으며, 인간은 실내온열환경에 대해 적응하며, 행동을 취해 자신의 쾌적한 환경을 유지해 왔다. 또한 사람들의 실내에서의 거주 및 업무 생활시간 증가 및 기후변화에 의한 지구온난화가 가속화 되면서 건물 냉⋅난방 가동시간 또한 계속 증가하고 있으며, 이는 건물에너지 소비에 직결된다. 이처럼 냉⋅난방 시스템 가동에 의한 건물의 에너지는 재실자의 쾌적성을 유지하기 위하여 소모되기 때문에 실내의 쾌적조건(쾌적온도)에 대한 이해는 건물 부문에서의 에너지 분석을 위해 필수적이다. 건물에너지 소비를 줄이는 것과 동시에 재실자의 열쾌적성을 확보하는 것도 매우 중요하다. 재실자의 건강 및 쾌적한 실내 환경을 조성하는 데 온열쾌적성은 매우 중요한 요소로 작용하기 때문에, 온열쾌적성을 평가하기 위한 새로운 열쾌적모델이 필요하다. 기존 연구에 따르면 Fanger의 열쾌적 모델(PMV 모델)로 예측되는 쾌적온도가 실제보다 다소 과장 되어 있다는 결과를 보이고 있으며, 이는 에너지 과소비의 주요 원인이 될 수 있다. 반면에 적응쾌적이론은 적응행동(Adaptive action)과 열적적응(Thermal adaptation)의 과정을 통해 재실자의 쾌적대(Comfort zone)가 증가한다는 현장실측 연구에 기반을 두고 있기 때문에, 이러한 재실자의 환경적응행동과 열쾌적성 변화 및 건물에너지 소비에 대한 상호 작용을 이해한다면, 에너지 사용을 예측하거나 공조시스템의 에너지 절약적인 운영을 제공하는 데 많은 도움이 될 것이다. 본 연구에서는 재실자의 환경적응행동을 고려한 적응쾌적모델을 개발하여 재실자의 열쾌적성을 향상시키고, 동시에 건물에너지 소비를 저감시키고자 한다. 이에 현장실측 및 주관적 설문평가를 기반으로 재실자의 환경적응행동이 열쾌적성에 미치는 영향을 파악하여, 환경적응행동을 고려한 적응쾌적모델을 개발 및 검증하였다. 또한 개발된 적응쾌적모델과 기존 모델을 비교하여 열쾌적성의 질적 개선 및 건물에너지 절감을 도모하고자 하였다. 재실자의 환경적응행동을 분석하기 위해 총 7개의 건물 내 8개 공간을 대상으로 현장 실측을 실시하였으며, 기존 실측 자료와 함께 실내외 열환경, 재실자의 열쾌적도 및 환경적응행동에 대한 기초 자료를 구축하였다. 환경적응행동에 따른 열쾌적성 평가에서 실내⋅외 온열환경 특성 분석을 하였다. 분석 결과를 바탕으로 물리적 요소, 행동적 적응, 심리적 적응, 종합적 요소와 열쾌적성의 관계를 평가하여 환경적응행동에 따른 열쾌적조건을 밝혀내고자 하였다. 기존 실측 데이터를 이용한 통계분석 과정을 통해 환경적응행동을 고려한 적응쾌적모델을 개발 및 검증하였다. 또한 검증 결과를 바탕으로 기존 실측데이터 및 새롭게 실측 된 경희대학교 열환경 및 열쾌적도 데이터를 통합하여 예측성능을 높이고, 다양한 건물특성을 반영할 수 적응쾌적모델을 최종 수정 및 완성하고자 하였다. 개발된 적응쾌적모델 적용 시 건물에너지 소비에 미치는 영향을 파악하기 위하여 EenrgyPlus 8.0을 사용하여 건물에너지 소비량을 분석하였다. 기존 모델(ASHRAE 기준)과 비교하여, 재실자의 환경적응행동을 고려한 적응쾌적모델의 에너지 저감량을 평가하였다. 재실자가 실제 쾌적감을 느끼는 쾌적온도는 다양한 환경변수의 복합적인 상호관계에 따라 달라 질 수 있다. 실측 결과 중성점온도(쾌적온도)가 재실자의 실제 온열감 반응과 가장 높은 상관성을 보였다. 또한 재실자의 행동적, 심리적 적응행동은 재실자의 온열감 및 쾌적감에 영향을 미치며, 이러한 환경적응행동을 고려한 적응쾌적모델을 개발을 통해 쾌적성을 제고하고, 건물에너지를 저감시킬 수 있을 것이다. 실외 지수가중치 평균온도(Trm)와 중성점온도(쾌적온도)와의 통계분석을 통하여 새로운 적응쾌적 모델을 개발하였으며, 통계적 유효성을 충족시켰다. 개발된 적응쾌적모델의 쾌적 범위(±2K)를 결정하고, 모델의 예측성능을 검증하였다. 적응쾌적모델의 예측성능 분석 결과, 쾌적온도 범위 내에 포함되는 비율이 경희대학교의 경우, 전체 기간 동안 61.6%, S사무소의 경우, 50.0%의 예측성능을 나타냈다. 따라서 적응쾌적모델의 예측성능을 높이기 위해 다양한 건물특성에 따른 현장상황을 반영하도록 하는 것이 필요하다. 기존 실측데이터를 모두 통합하여 적응쾌적모델을 최종 수정 및 완성하였다. 도출된 수정 적응쾌적모델은 Trm(실외 지수가중치 평균온도)과 중성점온도(쾌적온도)와의 관계에 대한 회귀식이 그 기반을 이루고 있으며, 냉방기간(Tc=0.210*Trm+20.833)과 난방기간(Tc=0.053*Trm+24.151)에 대한 수정 적응쾌적모델을 최종적으로 도출하였다. 따라서 최종적으로 도출된 수정 적응쾌적모델은 다양한 건물특성에 따른 현장 상황에 대응할 수 있고, 에너지 절약 성능도 제고할 수 있을 것이다. 냉방기간의 경우 쾌적모드, ASHRAE 기준, 표준모드, 에너지절약모드 순으로 냉방에너지 소비량이 높게 나타났다. 난방기간의 경우 쾌적모드, 표준모드, 에너지절약모드, ASHRAE 기준 순으로 난방에너지 소비량이 높게 나타났다. 냉방기간의 경우 적응쾌적모델의 에너지 절약모드 및 표준모드 적용시 건물에너지를 저감시킬 수 있으며, 난방기간에는 건물에너지 저감이 미비한 것으로 나타났다. 하지만 연간 건물에너지 소비량 결과를 보면, 적응쾌적모델의 에너지 절약모드가 가장 에너지 절감적인 방안으로 나타났다. 따라서 재실자의 쾌적성을 높이는 동시에 건물에너지 소비 절감을 고려한다면, 적응쾌적모델이 효과가 있는 것으로 사료된다.

한국 실버타운의 활성화를 위한 민간과 정부 부문의 역할에 관한 연구

조선호 경기대학교 2000 국내석사

퍼지논리를 이용한 로보트 매니퓰레이트의 위치 제어

조선호 경희대학교 대학원 1997 국내석사

음성인식을 위한 분할 비터비 알고리즘

조선호 高麗大學校 大學院 2011 국내석사

음성인식은 마이크로폰으로 소리신호를 입력 받아 그 소리신호가 어떤 음소, 단어, 문장인지 인식하는 기술이며 인간-컴퓨터간 음성 인터페이스를 구축하기 위해 매우 중요하다. 음성인식에서 입력은 마이크로폰으로 받은 음성신호를 컴퓨터로 처리 가능한 특징벡터로 변환하여 사용하고, 그 특징벡터는 인간의 청각신경과 비슷하도록 만든 mel-frequency cepstral coefficients (MFCC) 모델을 주로 이용한다. 음향모델은 기계학습 방법 중 하나인 hidden Markov model (HMM) 을 이용하여 학습과 인식에 주로 사용되고 최종 결과를 출력하는 디코딩에는 Viterbi algorithm이 주로 사용되고 있다. 현재 대부분의 음성인식기는 충분히 병렬처리 되지 못하고 있으나 멀티코어를 가진 컴퓨터의 증가로 병렬처리가 가능한 음성인식 알고리즘 연구의 필요성이 증대되고 있다. 본 논문은 음성인식 병렬처리에 활용될 수 있는 디코딩 알고리즘인 segmented Viterbi를 제안하고 실험하였다. 일반적인 Viterbi는 전체 음성구간에 대해 각각의 확률과 그 확률을 가지게 하는 바로 전의 state를 저장하고 최종적으로 backtracking과정을 통해 가장 확률이 높았던 path를 찾아 결과로 출력하는 음성인식 디코딩에 사용되는 알고리즘으로 이 과정이 한번에 처리되기 때문에 일반적으로는 병렬화 되기 어렵다. 그 문제를 해결하기 위해 제안한 segmented Viterbi algorithm은 기존과는 다르게 전체 음성구간을 한번에 디코딩 하는 것이 아니라 음성구간을 적당한 크기로 나누어 각각 디코딩하는 방법이다. Segmented Viterbi는 입력 받은 음성신호를 적당한 크기로 분할하고 각각 Viterbi 디코딩을 하여 음소형태로 결과를 출력한 다음 그 결과들을 취합하여 최종 인식 결과를 만든다. 이 segmented Viterbi algorithm의 성능 향상을 위해 고려될 문제로는 음성 분할 방법과 Viterbi 디코딩 후 결과 취합 방법, 그리고 정확도를 올리기 위한 추가적인 오류 보정 방법이 있다. 본 논문은 segmented Viterbi algorithm의 정확도 상승을 위해 여러 세부 특성을 가진 방법을 제안한다. 에너지가 큰 peak를 모음 중간으로 보고 그곳을 찾아 분할하는 방법, 위에서 찾은 모음 음소의 peak에서 가까운 valley를 찾아 음소의 경계로 보고 분할하는 방법, 분할하는 구간은 오류일 가능성이 높기 때문에 분할 구간에서 path backtracking시 나오는 첫 음소를 버리고 그 시간을 다음 구간에 반영하는 방법, 균등하게 분할하는 방법, 가능한 비슷한 구간을 만들지만 지역적인 valley에서 분할하는 방법, 가능한 비슷한 구간을 만들지만 지역적인 peak에서 분할하는 방법을 세부 특성으로 제안하였다. 제안된 방법은 monophone과 왼쪽만 이용한 cross-word biphone, cross-word triphone에서 실험을 수행 하였다. 추가적으로 path backtracking시 나오는 첫 음소를 버리고 그 시간을 다음 구간에 반영하는 오류 보정 방법에 대해 첫 음소뿐 아니라 여러 개의 음소를 버릴 때의 효과와, 제안한 방법들에 대한 monophone bigram에서의 영향도 실험하였다.

새싹인삼의 수경재배와 배추 무 고추의 자연과학농업 연구

조선호 건국대학교 대학원 2022 국내박사

인삼은 다년생 작물로서 수확할 수 있기까지는 4-6년의 재배 기간이 소요되며, 보통 밭 재배에서 인삼의 수확 후 휴작 기간이 10년 이상으로 다른 작물과 비교해 토지 생산성이 낮다. 생육 기간 같은 장소에서 자라기 때문에 토양 특성에 따른 영향을 많이 받는다. 최근 수경재배 방법을 이용한 인삼 재배가 시도되고 있음이 보고되고 있는데, 이 방법으로 인삼을 재배할 경우 생육 기간이 단축되고 휴작기간이 없으며 단위 면적당 생산성이 높아질 것으로 판단된다. 인삼은 주로 뿌리를 한약재 또는 건강 기능 식품원료로 이용하고 있는데, 수경재배로 재배된 인삼의 경우 쌈 채소, 잎 차 등 인삼의 이용 가능성이 증대된다는 점에서 많은 관심이 쏠리고 있다. 인삼의 잎은 뿌리와 비교하면 주요 약리 성분인 사포닌 함량이 높다고 보고되었다. 인삼의 생육은 고초균 > 방선균 >자생균 > 대조구 순으로 많이 생성되었으며, 진세노사이드 함량은 방선균 > 고초균 > 자생균 > 대조구 순으로 생성되었다. 양액의 온도가 19℃에서 인삼의 생육이 가장 좋게 나타났다. 양액의 온도에 따른 진세노사이드의 성분 함량은 높은 온도인 20.5℃와 19℃에서 17.5℃와 16℃에서 보다 더 높게 나타났다. 양액의 EC 농도가 390ds/m에서 줄기와 잎의 생육이 가장 좋았으며, 421ds/m에서 뿌리의 생육이 가장 좋았다. As a perennial crop, ginseng takes 4-6 years of cultivation before it can be harvested. In general, ginseng has a rest period of more than 10 years after harvest in field cultivation, which is low compared to other crops in terms of land productivity. Because it grows in the same place during the growing season, it is greatly affected by soil characteristics. Recently, it has been reported that ginseng cultivation using the hydroponics method is being attempted. If ginseng is cultivated in this way, the growth period will be shortened, there will be no rest period, and it is judged that the productivity per unit area will increase. Ginseng roots are mainly used as herbal medicines or health functional food raw materials,

警察의 地域社會 犯罪統制戰略에 관한 硏究 : 大邱廣域市를 中心으로

조선호 東國大學校 大學院 2002 국내박사

Purpose of the study During the past 30 years, numerous demonstration projects have highlighted the limitations of traditional police practices. Specifically, evaluators have challenged the effectiveness of rapid response strategies, routine criminal investigation, random motorized patrols, and traditional crime analyses. In the 1970s and 1980s, police research amplified the role of citizens in solving crime. Local residents became the primary source of information concerning neighborhood problems. In short, the realization that the police are very dependent on the cooperation of citizens to reduce crime and to improve public safety was a major impetus behind community based crime control strategy. Many of community based crime control strategy are beyond the traditional capacities and roles of officers, who were initially selected and trained to perform only the basic activities associated with law enforcement, such as patrol, investigation, order maintenance, arrest, and report writing. Community based crime control strategy requires officers to do many of their old jobs in innovative ways : It forces officers to attempt unfamiliar and challenging tasks, to identity and solve a broad range of problems, and to reach out to the community who were previously outside the policing. Moreover, Community based crime control strategy expects officers to control disorder which would lead to serous crime. The purpose of this study is to introduce community based crime control strategies in Korea to reduce growing public dissatisfactions with traditional police practices, and to respond to increasing demands for a new model of policing that can actually alleviate crime, disorder, and vandalism. Method This study used literature review, survey, and analysis of official records. In the literature review, this study screened the theoretical foundation of the previous studies, in particular, "Broken windows theory" & "Problem-oriented policing" to address the relation between disorder and serious crime. The survey also used the latest official police records The survey was conducted to 500 police officers (working for Daegu Police Agency & Stations), and 304 citizens (residing in the city of Daegu) in 2002. Interviewers distributed the questionnaire and conducted the survey. A total of 804 completed surveys were collected. The survey included questions that formed tens of scales measuring the various components of factors such as citizens' attitude toward police work (fear of crime, trivial disorder, etc) and officers' attitude toward police work (fear of crime, trivial disorder, community policing, etc) Findings The major findings of the survey is as follows; (1) There are differences in perception on crime rates in their community between citizens and officers. Citizens perceive that a large number of crimes happen in their neighborhood, while police officers perceive that they can suppress crimes effectively. (2) Both citizens and officers perceive that trivial disorders in the street would lead to serious crimes if trivial disorders are not prevented and controlled successfully in their initial stage. (3) Police officers have the passive attitude toward prevention of disorder in the street. They feel that they have little discretion and that they need their supervisor's order when they control disorder. (4) Police officers perceive that crime prevention activities are more important than any other police works. (5) Both citizens and officers perceive that police box system has a significant influence on crime prevention in the community. (6) There are differences in perception on which organization should coordinate police's crime prevention activities for the more effective administration within the police. Officers working for above police station consider the police station as a proper organization, while Officers working for police box think of the police box as a suitable organization. (7) There are differences in perception on the relationship between the frequency of personnel transfer and job consistency within the police. Officers above supervisor level think that they have little to do with, while Patrol officers think severely frequent personnel transfer lower the degree of job consistency. (8) There are differences in perception on what kind of patrol can prevent crime more effectively between citizens and officers. Citizens perceive that foot patrol is more effective than motor patrol, while police officers perceive that motor patrol is more effective than foot patrol. (9) Both citizens and officers perceive that 112 motor patrol has a significant influence on crime prevention in the community. (10) There are differences in perception on the police's response time to citizen's call between citizens and officers. Citizens perceive that the police do not respond promptly to their call, while police officers perceive that they respond swiftly to citizen's call. (11) There are differences in perception on effectiveness of the extensive roundup within the police. Officers above supervisor level consider it as a significant measure, while Patrol officers think of it as a insignificant measure. (12) There are differences in perception on effectiveness of the police practices based on computerized statistics within the police. Officers working with investigation division consider it as a helpful measure, while the other officers do not think of it as a helpful measure. (13) There are differences in perception on how affirmatively the citizen report crimes to the police between citizens and officers. Citizens perceive that they report crimes to the police negatively, while police officers perceive that the citizens report crimes to the police affirmatively. (14) There are differences in perception on how positively the citizen cooperate with the police between citizens and officers. Citizens perceive that they cooperate with the police positively, while police officers perceive that the citizens do not cooperate with the police positively. (15) There are differences in perception on how much the citizens' voluntary crime watch activity can help the police with crime prevention between citizens and officers. Citizens perceive that the citizens' voluntary crime watch activity is helping the police a great deal, while police officers perceive that the citizens' voluntary crime watch activity can give little help to the police. (16) Both citizens and officers perceive that there are not successful cooperation relation among the police, citizens, and private security. (17) Both citizens and officers perceive that private security (especially system security) has a great deal of positive influence on crime prevention. (18) Citizens perceive that they expect the police to play much more social roles beyond crime prevention and suppression. Conclusion Community based policing is the latest reform in law enforcement and is quite popular among politicians, citizens, and police managers. It evolved, in part, from a growing dissatisfaction with traditional police practices and a recognition of their shortcomings. The concept of community based crime control strategy is rather nebulous, and in the field, it assumes many forms, including foot patrol, autonomy, discretion, disorder control, and community organizing. Based on the results, I would like suggest some respects, as my conclusion of this study. (1) Unlike traditional police practices, community based crime control strategy should take disorder control into consideration because disorder would lead to serious crime eventually. The patrol officers should be given a reasonable discretion to wage. In addition, the level of autonomy of the police station should be raised. (2) Foot patrol is probably the most popular and widely implemented component of community based crime control strategy. Foot patrol can increase citizen-police contacts through community meetings, door-to-door visits, and other efforts. The police should build up foot patrol more frequently compared with in the present, though many police officers tend to like motor patrol better than foot patrol. (3) To create cooperative partnerships with the community, the police need a better understanding of the social forces that influence citizen participation in community life. The evaluation system of the patrol officers should be changed into multi-evaluation which deals with quantity aspect as well as quality aspect. (4) There are numerous reasons for citizen nonparticipation, including fear of retaliation by other offenders and distrust of the police and other service agencies. The police should strive to engender high level of citizen involvement in community based crime control strategy. Hence, to build a lasting partnership, the police need significant outreach efforts. (5) The relation between the police and the private security should be encourage to reduce crime and improve public safety. The police should take measures to increase the level of security employees' education. In addition, The police can consider introducing so called the certification program into machinery security to reduce high false alarm rate and to improve public safety.

소비자중심경영 인증제도(Consumer Centered Management: CCM)의 실행력 강화를 위한 개선방안 : 해외 평가제도 비교 연구 및 기업 사례 분석

조선호 서강대학교 경영전문대학원 2024 국내석사

Angelica decursiva 추출물에 의한 신경교종세포의 Apoptosis 유도

조선호 조선대학교 2010 국내석사

The Angelica decursiva has been used in Korean traditional medicine as an antitussive, an analgesic, an antipyretic and a cough remedy. However, it has been completely unknown its anti-tumor activity. In this study, therefore, we have examined the cytotoxic activity of ethanol extract of Angelica decursiva root (EEAD), and the mechanism of cell death exhibited by EEAD in C6 rat glioma cells. The cytotoxic effect of EEAD on cell growth inhibition in C6 cells was examined using MTT assay. And the cell death mechanism by EEAD was examined using DNA fragmentation analysis, immunoblotting and caspase activation measurement. Treatment of EEAD in C6 cells induced the apoptotic cell death in a concentration- and a time-dependent manner as determined by MTT assay and DNA fragmentation analysis. The proteolytic processing of caspase-3, -7 and -9 was increased by EEAD treatment in C6 cells. In addition, activation of caspase-3 and -7 was detected in living C6 cells by fluorescence microscopy. These results suggest that the EEAD can induce the suppression of cell growth and cell apoptosis in C6 rat glioma cells, and that it may have potential properties for anti-tumor drug discovery.

Dendropanax morbifera Lev. 메탄올 추출물에 의한 사람 구강암 세포 KB의 apoptosis 유도

조선호 조선대학교 2015 국내박사

ABSTRACT Induction of Apoptosis by Dendropanax morbifera Lev. Methanol Extract in KB Human Oral Cancer Cells Cho, Seon-Ho Advisor: Prof. Kim, Do Kyung, Ph.D. Department of Biodental Engineering, Graduate School of Chosun University Dendropanax morbifera is evergreen broad-leaved arboreous tree belongs to the araliaceae. The country of origin is mainly the South Korea. The scientific name is Dendropanax morbifera that component is based compound polyacetylenes. Recently, many researchs have been conducted about anti-cancer effects using Dendropanax morbifera in liver cancer, lung cancer, cervical cancer, and so on. However, the effects of Dendropanax morbifera on oral cancer is not clearly established. Therefore, the main goal of this study was to investigate the effect of Dendropanax morbifera extract on cell growth and apoptosis induction in oral cancer cells. To determine the effects of Dendropanax morbifera on cell growth and apoptosis induction in oral cancer cells, it was examined by inhibition of cell growth (MTT assay), nuclear staining with DAPI, DNA fragmentation analysis determination of caspase activation and immunoblotting in KB human oral cancer cells. The results were as follows. 1.Dendropanax morbifera methanol(DMME) extract induced the inhibition of cell growth depended on the Dendropanax morbifera extract treatment time and concentration in KB cells. 2.DMME observed the changing nuclear size and nuclear condensation in KB cells. 3. DMME induced the DNA fragmentation in KB cells. 4.DMME promoted proteolytic cleavages of procaspase-3, -7, -8 and -9 with the increases in the amount of cleaved caspase-3, -7,-8 and -9. 5. Cleaved PARP was increased by DMME in KB cells. These results suggest that Dendropanax morbifera can induce the suppression of cell growth through the cell apoptosis pathways in KB human oral cancer cells, and that it many have potential properties for anti-cancer drug discovery. KEY WORDS:Dendropanax morbifera, Anti-cancer therapy, Apoptosis, Cell death, KB human oral cancer cells.